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The map label for this gene is gcvT

Identifier: 226223949

GI number: 226223949

Start: 1356710

End: 1357798

Strand: Direct

Name: gcvT

Synonym: Lm4b_01356

Alternate gene names: 226223949

Gene position: 1356710-1357798 (Clockwise)

Preceding gene: 226223941

Following gene: 226223950

Centisome position: 46.58

GC content: 37.28

Gene sequence:

>1089_bases
ATGACAGAGTTACTAAAAACACCGATTCATCCACTTTATGCAAAATATGGTGCGAAAACCATTGATTTTGGTGGATGGGA
TTTACCGGTACAATTTGCTGGTATAAAAGCAGAACATGAGGCCGTGCGAACTGATGCAGGGCTATTTGATGTGTCTCACA
TGGGAGAAATTCTAGTAAAAGGACCGGATAGTACTTCTTATTTACAGTATTTGTTAACCAATGACATTGAAAAAATAAAA
ATCGGAAAAGCACAATATAATATTATGTGCTATGAAACTGGTGGAACAGTGGACGATTTAGTAGTTTACAAGAAATCAGA
AACGGAATACATACTTGTTGTCAATGCAGCAAATACGGATAAGGATTTTGAATGGATGGTTAAAAATATTCGCGGGGATG
TTTCTGTTACCAATGTATCTTCGGAATATGGACAATTAGCACTTCAAGGGCCAAACGCAGAAAAAATTCTCGCAAAACTA
ACGGATGTAGATTTAAGTTCGATTAGCTTCTTTGGATTTGTGGAGGATGCGGATGTAGCAGGAGTGAAAACAATCATTTC
AAGAAGTGGCTATACTGGAGAAGATGGATTTGAAATTTATATGCCAAGCGCTGATGCAGGAAAAGTTTTTGAGGCAATTT
TAGCTGAAGGAGTAGCTCCAATTGGTTTAGGTGCACGGGATACATTGCGCTTAGAGGCGGTACTTGCGCTTTATGGGCAA
GAATTGAGCAAGGACATTACACCACTTGAAGCAGGGCTTAATTTTGCTGTTAAATTAAAAAAAGAAGCAGATTTTATAGG
TAAAGAAGCGCTTATTAAACAAAAAGAAGTAGGGCTGAATAGAAAACTAGTTGGTATTGAATTAATTGAACGAGGTATTC
CGCGTCACGATTATCCAGTATTTCTAAATGAGGAAGAAATTGGCATTGTGACTTCTGGTACCCAATCACCCACACTTGGA
ACAAATATTGGTCTTGCTCTAATTGATACAGCTTACACAGAAATAGGTCAAGAAGTAGAAGTAGGTATAAGAAACAAAAA
AGTAAAAGCGAAAATAGTACCAACACCATTTTATAAACGCGCAAAGTAA

Upstream 100 bases:

>100_bases
AGGATGTTTACGGGACGCAACTCTGGAGTCATTTTTGTGTTACGACAGGGCTTATGAACCATATAAGCCTTTTTGTTATG
TGAAAAAGGAGGATATAATT

Downstream 100 bases:

>100_bases
AAAGGAGGAAGTAATATGGCAAAACATCGTTATTTACCAATGACAGAGCAAGATGAAAAGGAAATGCTTGATGTAATAGG
GGTTAAGTCAATTGATGACT

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTELLKTPIHPLYAKYGAKTIDFGGWDLPVQFAGIKAEHEAVRTDAGLFDVSHMGEILVKGPDSTSYLQYLLTNDIEKIK
IGKAQYNIMCYETGGTVDDLVVYKKSETEYILVVNAANTDKDFEWMVKNIRGDVSVTNVSSEYGQLALQGPNAEKILAKL
TDVDLSSISFFGFVEDADVAGVKTIISRSGYTGEDGFEIYMPSADAGKVFEAILAEGVAPIGLGARDTLRLEAVLALYGQ
ELSKDITPLEAGLNFAVKLKKEADFIGKEALIKQKEVGLNRKLVGIELIERGIPRHDYPVFLNEEEIGIVTSGTQSPTLG
TNIGLALIDTAYTEIGQEVEVGIRNKKVKAKIVPTPFYKRAK

Sequences:

>Translated_362_residues
MTELLKTPIHPLYAKYGAKTIDFGGWDLPVQFAGIKAEHEAVRTDAGLFDVSHMGEILVKGPDSTSYLQYLLTNDIEKIK
IGKAQYNIMCYETGGTVDDLVVYKKSETEYILVVNAANTDKDFEWMVKNIRGDVSVTNVSSEYGQLALQGPNAEKILAKL
TDVDLSSISFFGFVEDADVAGVKTIISRSGYTGEDGFEIYMPSADAGKVFEAILAEGVAPIGLGARDTLRLEAVLALYGQ
ELSKDITPLEAGLNFAVKLKKEADFIGKEALIKQKEVGLNRKLVGIELIERGIPRHDYPVFLNEEEIGIVTSGTQSPTLG
TNIGLALIDTAYTEIGQEVEVGIRNKKVKAKIVPTPFYKRAK
>Mature_361_residues
TELLKTPIHPLYAKYGAKTIDFGGWDLPVQFAGIKAEHEAVRTDAGLFDVSHMGEILVKGPDSTSYLQYLLTNDIEKIKI
GKAQYNIMCYETGGTVDDLVVYKKSETEYILVVNAANTDKDFEWMVKNIRGDVSVTNVSSEYGQLALQGPNAEKILAKLT
DVDLSSISFFGFVEDADVAGVKTIISRSGYTGEDGFEIYMPSADAGKVFEAILAEGVAPIGLGARDTLRLEAVLALYGQE
LSKDITPLEAGLNFAVKLKKEADFIGKEALIKQKEVGLNRKLVGIELIERGIPRHDYPVFLNEEEIGIVTSGTQSPTLGT
NIGLALIDTAYTEIGQEVEVGIRNKKVKAKIVPTPFYKRAK

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=369, Percent_Identity=33.8753387533875, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI257796258, Length=348, Percent_Identity=34.1954022988506, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI24797151, Length=335, Percent_Identity=33.134328358209, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI257796254, Length=362, Percent_Identity=31.4917127071823, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI257796256, Length=311, Percent_Identity=33.4405144694534, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI197927446, Length=282, Percent_Identity=28.7234042553192, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21361378, Length=282, Percent_Identity=28.7234042553192, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194306651, Length=325, Percent_Identity=25.8461538461538, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1789272, Length=369, Percent_Identity=40.3794037940379, Blast_Score=254, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI17560118, Length=362, Percent_Identity=35.9116022099448, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI32563613, Length=317, Percent_Identity=28.7066246056782, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71994045, Length=343, Percent_Identity=26.8221574344023, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71994052, Length=343, Percent_Identity=26.8221574344023, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320222, Length=392, Percent_Identity=35.2040816326531, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI20129441, Length=372, Percent_Identity=35.4838709677419, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI28571104, Length=297, Percent_Identity=31.3131313131313, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI20130091, Length=362, Percent_Identity=26.7955801104972, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 39565; Mature: 39434

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELLKTPIHPLYAKYGAKTIDFGGWDLPVQFAGIKAEHEAVRTDAGLFDVSHMGEILVK
CCCHHHCCCHHHHHHHCCCEEECCCCCCEEEEECCCCHHHHHHCCCCEEEHHCCCEEEEE
GPDSTSYLQYLLTNDIEKIKIGKAQYNIMCYETGGTVDDLVVYKKSETEYILVVNAANTD
CCCCHHHHHHHHHCCCCCEEECCEEEEEEEEECCCCEEHEEEEEECCCCEEEEEECCCCC
KDFEWMVKNIRGDVSVTNVSSEYGQLALQGPNAEKILAKLTDVDLSSISFFGFVEDADVA
CHHHHHHHHHCCCEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCHH
GVKTIISRSGYTGEDGFEIYMPSADAGKVFEAILAEGVAPIGLGARDTLRLEAVLALYGQ
HHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
ELSKDITPLEAGLNFAVKLKKEADFIGKEALIKQKEVGLNRKLVGIELIERGIPRHDYPV
HHHCCCCHHHHCCCEEEEEECHHHHCCHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCE
FLNEEEIGIVTSGTQSPTLGTNIGLALIDTAYTEIGQEVEVGIRNKKVKAKIVPTPFYKR
EECCCCEEEEECCCCCCCCCCCCCCEEEHHHHHHCCCCEEECCCCCEEEEEECCCCCCCC
AK
CC
>Mature Secondary Structure 
TELLKTPIHPLYAKYGAKTIDFGGWDLPVQFAGIKAEHEAVRTDAGLFDVSHMGEILVK
CCHHHCCCHHHHHHHCCCEEECCCCCCEEEEECCCCHHHHHHCCCCEEEHHCCCEEEEE
GPDSTSYLQYLLTNDIEKIKIGKAQYNIMCYETGGTVDDLVVYKKSETEYILVVNAANTD
CCCCHHHHHHHHHCCCCCEEECCEEEEEEEEECCCCEEHEEEEEECCCCEEEEEECCCCC
KDFEWMVKNIRGDVSVTNVSSEYGQLALQGPNAEKILAKLTDVDLSSISFFGFVEDADVA
CHHHHHHHHHCCCEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCHH
GVKTIISRSGYTGEDGFEIYMPSADAGKVFEAILAEGVAPIGLGARDTLRLEAVLALYGQ
HHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
ELSKDITPLEAGLNFAVKLKKEADFIGKEALIKQKEVGLNRKLVGIELIERGIPRHDYPV
HHHCCCCHHHHCCCEEEEEECHHHHCCHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCE
FLNEEEIGIVTSGTQSPTLGTNIGLALIDTAYTEIGQEVEVGIRNKKVKAKIVPTPFYKR
EECCCCEEEEECCCCCCCCCCCCCCEEEHHHHHHCCCCEEECCCCCEEEEEECCCCCCCC
AK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]