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The map label for this gene is pnpA

Identifier: 226223932

GI number: 226223932

Start: 1343040

End: 1345211

Strand: Direct

Name: pnpA

Synonym: Lm4b_01339

Alternate gene names: 226223932

Gene position: 1343040-1345211 (Clockwise)

Preceding gene: 226223931

Following gene: 226223936

Centisome position: 46.11

GC content: 39.87

Gene sequence:

>2172_bases
ATGTCTGAAAAACAAGTATTTTCAACAGAATGGGCAGGTAAGACATTATCTGTCGAAGTAGGTCAATTAGCAAAACAAGC
AAGTGGAGCAGCCTTAATTCGTTACGGCGATACGGTCGTTTTAACAGCGGCAGTAGGTTCTAAAAAACCACGTCCAGGCG
ACTTTTTCCCACTAACAGTTAACTATGAAGAAAAAATGTACTCCGTTGGTAAAGTTCCCGGTGGATTTTTAAAACGTGAA
GGACGTCCTAGTGACCGTGCGACATTAACAGCGCGTCTAATTGACCGTCCAATTCGTCCATTATTTGCAGAAGGTTTCCG
TAATGAAGTTCAAATTACATCTACTGTTTTCAGTGTGGATCAAGATTGTTCTCCAGAAATGGCAGCAATGCTTGGCTCTT
CTGTTGCATTAGTTATTTCCGATATTCCATTTGAAGGACCGATCGCTGGTGTGGATGTGGGTCGTATCGATGGAAAATAC
GTTATCAACCCAACTATCGAACAAGCAGAAAAAAGTGATATTAGCTTAACCGTTGCAGGAACTTATGATGCAATCAACAT
GGTAGAAGCTGGAGCGAAAGAAGTTTCAGAAGAAGCAATGCTTGAAGCAATCATGTTCGGTCACGAAGAAATTAAACGTC
TTTGTGAATTCCAACAACAAATTATCGCTGCAGTTGGTAAAGAAAAACGCGAAATTGAACTTTTCGTAAGCGATCCTGAA
CTTGAAGCAGAAGTAAAAGCAGCAAGTGAAGGCAAAATGAAAGCAGCTATCAAAACAGAAGAGAAAAAAGCACGTGAAGC
AGCAATTGAAGATGTAAAAGAAGAAATTTTAGAAAGCTATAAAGCAAAAGAACTAGAAAACGAAGCAGAAATTCTTAGCG
AAGTAGCTCATATTCTTGAAATGATCGAAAAAGACGAAATGCGTCGTTTGATTTCTCAAGATAAAATCCGTCCAGATGGT
CGTAAAGTAAACGAAATTCGTCCACTTTCTTCTGAAGTTGGTATGCTTCCTCGTGTCCATGGTTCAGGTTTATTCACTCG
TGGTCAAACACAAGCTCTAAGTGTATGTACACTGGCACCGCTTCGTGAACACCAAATCATTGATGGTTTAGGGACAGAAG
AGTACAAACGCTTTATGCATCATTATAACTTCCCGCAATTTAGTGTTGGGGAAACAGGTCCTCGTCGTGCTCCAGGCCGT
CGTGAAATCGGTCACGGTGCATTAGGTGAACGTGCGTTACAATATGTTATTCCTTCCGAAGAGGAATTCCCGTACACAAT
CCGTTTAGTATCTGAAGTTCTTGAATCTAATGGCTCTAGTTCGCAAGCAAGTATTTGTGGTTCTACACTTGCAATGCTTG
ATGCTGGTGTTCCAATTAAAGCGCCAGTTGCAGGTATCGCAATGGGTCTTGTCAAACTTGGCGATGATTACACCATTCTT
TCTGATATCCAAGGTATGGAAGATCACTTTGGCGATATGGACTTTAAAGTTGCTGGAACAAAAGATGGTATCACAGCACT
TCAAATGGACATCAAGATTGATGGCTTAAGCCGCCAAATTTTGGACGAAGCATTAACGCAAGCAAAAGAAGGGCGCCTAC
ACATTCTAGAACACTTGACTAGCACAATTAGCGCACCACGTGAAGAACTTTCTGCTTATGCTCCAAAAATCATTACACTT
AACATCAAACCTGAGAAAATCAAAGATGTTATTGGACCTGGCGGAAAACAAATCAATGCAATTATTGATGAAACTGGCGT
AAAAATTGATATCGAACAAGATGGTACAGTGTACATTGCTTCTCAAGATCAAGCAATGAACCGTAAAGCAATTGCTATCA
TCGAAGACATCGTTCGTGAAGTGGAAGTGGGAGAAGTTTATACTGGAAAAGTTCGTCGTATCGAAAAATTTGGCGCATTT
GTTGAATTATTCAAAGGTACAGATGGCTTAGTTCACATTTCTGAATTAGCACATGAACGTGTTGGTAAAGTAGAAGATAT
CCTAAAACTAGGTGATGAAGTTACTGTTAAAGTTATCGAAGTAGACCAACAAGGTCGCGTTAACTTATCACGTAAAGCTT
TGCTTGAGAAAAAAGAACAACCAGAAGGCGATAAAAAACCACAAGCAGAGAAAAAATTCTATCCTAAAACGAAAAAACCA
GAATCTAAATAA

Upstream 100 bases:

>100_bases
GGATTCATGTGTGCTTTAGAAAATCTAAAGTGTGCGTGAACATTCTTATGCATGGTAAAGTTTCTGGTTCGTGTTTTTCC
TAATCAAAGGAGATTAAAAT

Downstream 100 bases:

>100_bases
TACAAAAAGCAAGTAGCCTGAGCTACTTGCTTTTTTTACTATTTTTTTGGACGTGTTTTTAAATGTGTCCGAAGTGATTT
TTGAATAACTTTCCAGCGGT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MSEKQVFSTEWAGKTLSVEVGQLAKQASGAALIRYGDTVVLTAAVGSKKPRPGDFFPLTVNYEEKMYSVGKVPGGFLKRE
GRPSDRATLTARLIDRPIRPLFAEGFRNEVQITSTVFSVDQDCSPEMAAMLGSSVALVISDIPFEGPIAGVDVGRIDGKY
VINPTIEQAEKSDISLTVAGTYDAINMVEAGAKEVSEEAMLEAIMFGHEEIKRLCEFQQQIIAAVGKEKREIELFVSDPE
LEAEVKAASEGKMKAAIKTEEKKAREAAIEDVKEEILESYKAKELENEAEILSEVAHILEMIEKDEMRRLISQDKIRPDG
RKVNEIRPLSSEVGMLPRVHGSGLFTRGQTQALSVCTLAPLREHQIIDGLGTEEYKRFMHHYNFPQFSVGETGPRRAPGR
REIGHGALGERALQYVIPSEEEFPYTIRLVSEVLESNGSSSQASICGSTLAMLDAGVPIKAPVAGIAMGLVKLGDDYTIL
SDIQGMEDHFGDMDFKVAGTKDGITALQMDIKIDGLSRQILDEALTQAKEGRLHILEHLTSTISAPREELSAYAPKIITL
NIKPEKIKDVIGPGGKQINAIIDETGVKIDIEQDGTVYIASQDQAMNRKAIAIIEDIVREVEVGEVYTGKVRRIEKFGAF
VELFKGTDGLVHISELAHERVGKVEDILKLGDEVTVKVIEVDQQGRVNLSRKALLEKKEQPEGDKKPQAEKKFYPKTKKP
ESK

Sequences:

>Translated_723_residues
MSEKQVFSTEWAGKTLSVEVGQLAKQASGAALIRYGDTVVLTAAVGSKKPRPGDFFPLTVNYEEKMYSVGKVPGGFLKRE
GRPSDRATLTARLIDRPIRPLFAEGFRNEVQITSTVFSVDQDCSPEMAAMLGSSVALVISDIPFEGPIAGVDVGRIDGKY
VINPTIEQAEKSDISLTVAGTYDAINMVEAGAKEVSEEAMLEAIMFGHEEIKRLCEFQQQIIAAVGKEKREIELFVSDPE
LEAEVKAASEGKMKAAIKTEEKKAREAAIEDVKEEILESYKAKELENEAEILSEVAHILEMIEKDEMRRLISQDKIRPDG
RKVNEIRPLSSEVGMLPRVHGSGLFTRGQTQALSVCTLAPLREHQIIDGLGTEEYKRFMHHYNFPQFSVGETGPRRAPGR
REIGHGALGERALQYVIPSEEEFPYTIRLVSEVLESNGSSSQASICGSTLAMLDAGVPIKAPVAGIAMGLVKLGDDYTIL
SDIQGMEDHFGDMDFKVAGTKDGITALQMDIKIDGLSRQILDEALTQAKEGRLHILEHLTSTISAPREELSAYAPKIITL
NIKPEKIKDVIGPGGKQINAIIDETGVKIDIEQDGTVYIASQDQAMNRKAIAIIEDIVREVEVGEVYTGKVRRIEKFGAF
VELFKGTDGLVHISELAHERVGKVEDILKLGDEVTVKVIEVDQQGRVNLSRKALLEKKEQPEGDKKPQAEKKFYPKTKKP
ESK
>Mature_722_residues
SEKQVFSTEWAGKTLSVEVGQLAKQASGAALIRYGDTVVLTAAVGSKKPRPGDFFPLTVNYEEKMYSVGKVPGGFLKREG
RPSDRATLTARLIDRPIRPLFAEGFRNEVQITSTVFSVDQDCSPEMAAMLGSSVALVISDIPFEGPIAGVDVGRIDGKYV
INPTIEQAEKSDISLTVAGTYDAINMVEAGAKEVSEEAMLEAIMFGHEEIKRLCEFQQQIIAAVGKEKREIELFVSDPEL
EAEVKAASEGKMKAAIKTEEKKAREAAIEDVKEEILESYKAKELENEAEILSEVAHILEMIEKDEMRRLISQDKIRPDGR
KVNEIRPLSSEVGMLPRVHGSGLFTRGQTQALSVCTLAPLREHQIIDGLGTEEYKRFMHHYNFPQFSVGETGPRRAPGRR
EIGHGALGERALQYVIPSEEEFPYTIRLVSEVLESNGSSSQASICGSTLAMLDAGVPIKAPVAGIAMGLVKLGDDYTILS
DIQGMEDHFGDMDFKVAGTKDGITALQMDIKIDGLSRQILDEALTQAKEGRLHILEHLTSTISAPREELSAYAPKIITLN
IKPEKIKDVIGPGGKQINAIIDETGVKIDIEQDGTVYIASQDQAMNRKAIAIIEDIVREVEVGEVYTGKVRRIEKFGAFV
ELFKGTDGLVHISELAHERVGKVEDILKLGDEVTVKVIEVDQQGRVNLSRKALLEKKEQPEGDKKPQAEKKFYPKTKKPE
SK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=710, Percent_Identity=37.4647887323944, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI9506689, Length=227, Percent_Identity=27.3127753303965, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=50.6512301013025, Blast_Score=686, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=730, Percent_Identity=33.5616438356164, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6320850, Length=94, Percent_Identity=38.2978723404255, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281362905, Length=708, Percent_Identity=36.2994350282486, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651641, Length=708, Percent_Identity=36.2994350282486, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651643, Length=708, Percent_Identity=36.2994350282486, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI161079377, Length=659, Percent_Identity=35.8118361153263, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 79543; Mature: 79412

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKQVFSTEWAGKTLSVEVGQLAKQASGAALIRYGDTVVLTAAVGSKKPRPGDFFPLTV
CCCCCEEECCCCCCEEEEEHHHHHHHCCCCEEEEECCEEEEEEECCCCCCCCCCEEEEEE
NYEEKMYSVGKVPGGFLKREGRPSDRATLTARLIDRPIRPLFAEGFRNEVQITSTVFSVD
CHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEHHHEECC
QDCSPEMAAMLGSSVALVISDIPFEGPIAGVDVGRIDGKYVINPTIEQAEKSDISLTVAG
CCCCHHHHHHHCCCEEEEEECCCCCCCCCCCEECCCCCEEEECCCHHHCCCCCEEEEEEC
TYDAINMVEAGAKEVSEEAMLEAIMFGHEEIKRLCEFQQQIIAAVGKEKREIELFVSDPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
LEAEVKAASEGKMKAAIKTEEKKAREAAIEDVKEEILESYKAKELENEAEILSEVAHILE
CCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MIEKDEMRRLISQDKIRPDGRKVNEIRPLSSEVGMLPRVHGSGLFTRGQTQALSVCTLAP
HHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHCCCCCCCCCCCEECCCCHHHHHHHHCC
LREHQIIDGLGTEEYKRFMHHYNFPQFSVGETGPRRAPGRREIGHGALGERALQYVIPSE
CHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
EEFPYTIRLVSEVLESNGSSSQASICGSTLAMLDAGVPIKAPVAGIAMGLVKLGDDYTIL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEH
SDIQGMEDHFGDMDFKVAGTKDGITALQMDIKIDGLSRQILDEALTQAKEGRLHILEHLT
HHHCCHHHHCCCCCEEEECCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHH
STISAPREELSAYAPKIITLNIKPEKIKDVIGPGGKQINAIIDETGVKIDIEQDGTVYIA
HHHHCCHHHHHHHCCEEEEEECCHHHHHHHHCCCCCEEHHEEECCCCEEEEECCCEEEEE
SQDQAMNRKAIAIIEDIVREVEVGEVYTGKVRRIEKFGAFVELFKGTDGLVHISELAHER
CCCCHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
VGKVEDILKLGDEVTVKVIEVDQQGRVNLSRKALLEKKEQPEGDKKPQAEKKFYPKTKKP
HHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCC
ESK
CCC
>Mature Secondary Structure 
SEKQVFSTEWAGKTLSVEVGQLAKQASGAALIRYGDTVVLTAAVGSKKPRPGDFFPLTV
CCCCEEECCCCCCEEEEEHHHHHHHCCCCEEEEECCEEEEEEECCCCCCCCCCEEEEEE
NYEEKMYSVGKVPGGFLKREGRPSDRATLTARLIDRPIRPLFAEGFRNEVQITSTVFSVD
CHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEHHHEECC
QDCSPEMAAMLGSSVALVISDIPFEGPIAGVDVGRIDGKYVINPTIEQAEKSDISLTVAG
CCCCHHHHHHHCCCEEEEEECCCCCCCCCCCEECCCCCEEEECCCHHHCCCCCEEEEEEC
TYDAINMVEAGAKEVSEEAMLEAIMFGHEEIKRLCEFQQQIIAAVGKEKREIELFVSDPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
LEAEVKAASEGKMKAAIKTEEKKAREAAIEDVKEEILESYKAKELENEAEILSEVAHILE
CCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MIEKDEMRRLISQDKIRPDGRKVNEIRPLSSEVGMLPRVHGSGLFTRGQTQALSVCTLAP
HHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHCCCCCCCCCCCEECCCCHHHHHHHHCC
LREHQIIDGLGTEEYKRFMHHYNFPQFSVGETGPRRAPGRREIGHGALGERALQYVIPSE
CHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
EEFPYTIRLVSEVLESNGSSSQASICGSTLAMLDAGVPIKAPVAGIAMGLVKLGDDYTIL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEH
SDIQGMEDHFGDMDFKVAGTKDGITALQMDIKIDGLSRQILDEALTQAKEGRLHILEHLT
HHHCCHHHHCCCCCEEEECCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHH
STISAPREELSAYAPKIITLNIKPEKIKDVIGPGGKQINAIIDETGVKIDIEQDGTVYIA
HHHHCCHHHHHHHCCEEEEEECCHHHHHHHHCCCCCEEHHEEECCCCEEEEECCCEEEEE
SQDQAMNRKAIAIIEDIVREVEVGEVYTGKVRRIEKFGAFVELFKGTDGLVHISELAHER
CCCCHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
VGKVEDILKLGDEVTVKVIEVDQQGRVNLSRKALLEKKEQPEGDKKPQAEKKFYPKTKKP
HHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCC
ESK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]