Click here to switch to the map view.

The map label for this gene is tig

Identifier: 226223870

GI number: 226223870

Start: 1276562

End: 1277845

Strand: Direct

Name: tig

Synonym: Lm4b_01277

Alternate gene names: 226223870

Gene position: 1276562-1277845 (Clockwise)

Preceding gene: 226223869

Following gene: 226223871

Centisome position: 43.83

GC content: 37.69

Gene sequence:

>1284_bases
ATGTCAGTAAAATGGGAAAAACAAGAAGGTAATGTTGGAAAACTTACTTTTGAAATTGAACAAGAAAAAGTAAAAGAAGG
TTTAGATAGAGCTTTCGTAAAAGTGCGTAAAACTCTTAATGTGCCTGGCTTCCGTAAAGGGAAAGTTCCACGTCAAATTT
TCAATCAACGTTTTGGTGAAGAAGCTCTTTTCCAAGATGCGCTTGATATCTTGCTTCCAGAAGTATATTCTGCAGCAATT
GACGAAGCTGGTATTGATCCAGTGGATACTCCTCAAGTAAACATTGAGTCTATGGAAAAAGGCGAAACTTGGGTTTTAAC
AGCAGAAGTTACTGTAAAACCTGAAGTGAAACTTGGAGATTACAAAGGTCTTGAAGTAGAAAAACGTGAAACAGAACTTA
CAACAGAAGAATTAGAAGCGGAATTAAAACAATTACAAGAGCGTCAAGCTGAATTAGTTGTTAAAGAAGATGCACCAGCT
GAAAACGGTGACACTGTTATCCTTGATTTTGAAGGATTTAAAGACGGCGTAGCTTTCGAAGGTGGACAAGCTGAAAATCA
TTCTCTAGAACTTGGAAGCGGACAATTCATTCCTGGTTTTGAAGAAAAATTAGTTGGCTTAAAAGCTGGCGACGAAGCGG
ATATCGAGCTAACTTTCCCGGAAGAATATCATGCGGAAGATTTAGCTGGACAACCTGTAGTTTTCAAAGTGAAATTACAC
GAAATCAAAACAAAAGAAGTTCCAGCTCTTGACGATGAGCTTGCAAAAGACATCGACGAAGAAGTAGAAACTTTAGATGA
ATTAAAAGAAAAAATCTCTAAACGTTTACAAGAAGCAAAAGAAGAATCTGTTGCTCAAGCTAAACAAGAAGAAGTTATTG
CGAAAGCTGTTGAAAATGCAGAAGTGGACATTCCACACGCAATGGTTCACCATGAAGCTGATCATTTAATGAATCACTTT
GCACAAGATTTACAAGCACAAGGTCTAACTCCTGAATTATATTACCAATTCACTGGCCAAACAGAAGAAGCTATGCACGC
ACAAATGGAAAAAGACGCTGAAAAACGCGTGAAAATGAACTTAGTGCTTGAAGCAATTGCGGAAGCTGAAAACATTGAGC
CAACGGAAGAAGCTATTGACGAAGAAATTTCTACATTAGCTGAAAAATATGGTATGGAAAAAGACGCAGTTCGTGCTGCA
CTTGGCGATATGAGCGAACTTAAATCTGATCTTAAAATCCGCAAAGCTATCGACGTTTTATTAGATAGTGCAGTGGAAAA
ATAA

Upstream 100 bases:

>100_bases
TTCAAGTCAGGGATATCTGTGTTATTATTAGTGAGTATGGCTGAAACTTGTCATGAGAGTAATTAAATTGAACTAAATAG
ATGATGGAGGGAATTATACC

Downstream 100 bases:

>100_bases
GCTACGACTGATTTAATAATTTCATTAAAAAATGGGGGGCATGAGTTATACTCGTGTCCTTCTGAATGTAGAAATATGAA
TACAGATGATTGGCATTTCT

Product: trigger factor

Products: peptidylproline (omega=0)

Alternate protein names: TF [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSVKWEKQEGNVGKLTFEIEQEKVKEGLDRAFVKVRKTLNVPGFRKGKVPRQIFNQRFGEEALFQDALDILLPEVYSAAI
DEAGIDPVDTPQVNIESMEKGETWVLTAEVTVKPEVKLGDYKGLEVEKRETELTTEELEAELKQLQERQAELVVKEDAPA
ENGDTVILDFEGFKDGVAFEGGQAENHSLELGSGQFIPGFEEKLVGLKAGDEADIELTFPEEYHAEDLAGQPVVFKVKLH
EIKTKEVPALDDELAKDIDEEVETLDELKEKISKRLQEAKEESVAQAKQEEVIAKAVENAEVDIPHAMVHHEADHLMNHF
AQDLQAQGLTPELYYQFTGQTEEAMHAQMEKDAEKRVKMNLVLEAIAEAENIEPTEEAIDEEISTLAEKYGMEKDAVRAA
LGDMSELKSDLKIRKAIDVLLDSAVEK

Sequences:

>Translated_427_residues
MSVKWEKQEGNVGKLTFEIEQEKVKEGLDRAFVKVRKTLNVPGFRKGKVPRQIFNQRFGEEALFQDALDILLPEVYSAAI
DEAGIDPVDTPQVNIESMEKGETWVLTAEVTVKPEVKLGDYKGLEVEKRETELTTEELEAELKQLQERQAELVVKEDAPA
ENGDTVILDFEGFKDGVAFEGGQAENHSLELGSGQFIPGFEEKLVGLKAGDEADIELTFPEEYHAEDLAGQPVVFKVKLH
EIKTKEVPALDDELAKDIDEEVETLDELKEKISKRLQEAKEESVAQAKQEEVIAKAVENAEVDIPHAMVHHEADHLMNHF
AQDLQAQGLTPELYYQFTGQTEEAMHAQMEKDAEKRVKMNLVLEAIAEAENIEPTEEAIDEEISTLAEKYGMEKDAVRAA
LGDMSELKSDLKIRKAIDVLLDSAVEK
>Mature_426_residues
SVKWEKQEGNVGKLTFEIEQEKVKEGLDRAFVKVRKTLNVPGFRKGKVPRQIFNQRFGEEALFQDALDILLPEVYSAAID
EAGIDPVDTPQVNIESMEKGETWVLTAEVTVKPEVKLGDYKGLEVEKRETELTTEELEAELKQLQERQAELVVKEDAPAE
NGDTVILDFEGFKDGVAFEGGQAENHSLELGSGQFIPGFEEKLVGLKAGDEADIELTFPEEYHAEDLAGQPVVFKVKLHE
IKTKEVPALDDELAKDIDEEVETLDELKEKISKRLQEAKEESVAQAKQEEVIAKAVENAEVDIPHAMVHHEADHLMNHFA
QDLQAQGLTPELYYQFTGQTEEAMHAQMEKDAEKRVKMNLVLEAIAEAENIEPTEEAIDEEISTLAEKYGMEKDAVRAAL
GDMSELKSDLKIRKAIDVLLDSAVEK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=393, Percent_Identity=31.0432569974555, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: 5.2.1.8

Molecular weight: Translated: 47854; Mature: 47723

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKWEKQEGNVGKLTFEIEQEKVKEGLDRAFVKVRKTLNVPGFRKGKVPRQIFNQRFGE
CCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCH
EALFQDALDILLPEVYSAAIDEAGIDPVDTPQVNIESMEKGETWVLTAEVTVKPEVKLGD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEEEEEEEECCCEEECC
YKGLEVEKRETELTTEELEAELKQLQERQAELVVKEDAPAENGDTVILDFEGFKDGVAFE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGQAENHSLELGSGQFIPGFEEKLVGLKAGDEADIELTFPEEYHAEDLAGQPVVFKVKLH
CCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCCEEEECCHHHCHHHCCCCCEEEEEEEE
EIKTKEVPALDDELAKDIDEEVETLDELKEKISKRLQEAKEESVAQAKQEEVIAKAVENA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVDIPHAMVHHEADHLMNHFAQDLQAQGLTPELYYQFTGQTEEAMHAQMEKDAEKRVKMN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHH
LVLEAIAEAENIEPTEEAIDEEISTLAEKYGMEKDAVRAALGDMSELKSDLKIRKAIDVL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
LDSAVEK
HHHHHCC
>Mature Secondary Structure 
SVKWEKQEGNVGKLTFEIEQEKVKEGLDRAFVKVRKTLNVPGFRKGKVPRQIFNQRFGE
CCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCH
EALFQDALDILLPEVYSAAIDEAGIDPVDTPQVNIESMEKGETWVLTAEVTVKPEVKLGD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEEEEEEEECCCEEECC
YKGLEVEKRETELTTEELEAELKQLQERQAELVVKEDAPAENGDTVILDFEGFKDGVAFE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGQAENHSLELGSGQFIPGFEEKLVGLKAGDEADIELTFPEEYHAEDLAGQPVVFKVKLH
CCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCCEEEECCHHHCHHHCCCCCEEEEEEEE
EIKTKEVPALDDELAKDIDEEVETLDELKEKISKRLQEAKEESVAQAKQEEVIAKAVENA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVDIPHAMVHHEADHLMNHFAQDLQAQGLTPELYYQFTGQTEEAMHAQMEKDAEKRVKMN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHH
LVLEAIAEAENIEPTEEAIDEEISTLAEKYGMEKDAVRAALGDMSELKSDLKIRKAIDVL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
LDSAVEK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (omega=180)

Specific reaction: peptidylproline (omega=180) = peptidylproline (omega=0)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]