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The map label for this gene is flhF [H]

Identifier: 226223312

GI number: 226223312

Start: 724933

End: 726156

Strand: Direct

Name: flhF [H]

Synonym: Lm4b_00709

Alternate gene names: 226223312

Gene position: 724933-726156 (Clockwise)

Preceding gene: 226223311

Following gene: 226223313

Centisome position: 24.89

GC content: 42.24

Gene sequence:

>1224_bases
ATGGGAAAACATTTAGTAGAAAAGATGGAAATTTTTAAGGCGAACTCCAAGCGAGAAATACATAAAAAAATTCGCTTAGT
CACGAATGAGCCATATAAAATTACGGATGAACGTGTCACAAAGCTTGGGATTTTTAAGAAACAGTATGAAGTGACGGCGG
TCATTATGAGTGAAGTGGCGATAGCAGATGGTCGAATGGACTTTCAAGAAACATTTCAAAAATCGGTTGTCAAAAACAGA
CCGAAAACAGACGACTTATTAAAAAAAGAAAAATTACTAGAAATGCTTGCTGCGGGAGCGGAACTAGCACAGTCAACGCC
GCTTTTAGAAGAACGAAAAACACAGGAAGAAGAATTGTCAGCGATGCGTCTCGAACTAGCGGCTTTAAACCGCGAGCTTG
CAGTGAAAATGCGCGAGGAGCGTGAACAAAACAGTGATTTCGTGAAGTTTTTGAAGGGTCGCGGAATTAGTGATACGTAT
GTGGCGGACTTCATGCAGGCTGGGCGGAAGCAGTTTAAGCAAGTAGAAACGGCGCATTTGGATGATATTACAGATTGGTT
CGTTCCTTATTTCTCCGGAAAATTAGCGGTGGAAGATTCGTTTGATTTAAGCAACCACCGGATTATTTCTTTGATTGGGC
AAACGGGTGTTGGGAAAACGACCACGCTTGTGAAACTTGGATGGCAGCTGTTAAAGCAAAATCGGACAGTGGGCTTTATC
ACTACAGATACTTTTCGGTCAGGCGCAGTGGAGCAGTTCCAAGGTTATGCGGACAAACTCGATGCGGAGCTTATCGTTGC
GACGAGCCCGGCTGAACTAGAAGAAGCTGTGCAGTATATGACGTATGTTAACTGCGTGGATCATATTTTGATTGATACGG
TTGGGAGAAATTACTTAGCCGAGGAATCAGTGAGTGAAATTTCTGCCTATACCGATGTTGTTCATCCGGACTTAACGTGT
TTTACATTTTCTTCTGGAATGAAAAGTGCGGATGTGATGACGATTTTACCAAAACTAGCAGAAATTCCAATCGATGGTTT
TATCATTACAAAAATGGATGAAACGACGCGTATTGGTGATTTATACACGGTGATGCAAGAAACAAATTTGCCGGTGTTAT
ACATGACAGATGGACAAAATATTACGGAAAATATTTTCAGACCAAAAAGTCGTTGGTTAGCGGAGCGGTTTGTTGGGACA
GATAGAAGGCGGGTGCTGGAATGA

Upstream 100 bases:

>100_bases
GGACGTGCCGGTGCTCTCCATTAGTGAACTGTCGCCGGAAACAGAAGTGGAGCAAATTGCACTGATTGAACCAACTTGAT
GAGGGAGGTAATCCGTAGAA

Downstream 100 bases:

>100_bases
AGGGGTTATACATTGGCGCAGCAGGGATGATGAACTACATGCAACATATTCAAGTTCATTCTAATAACGTGGCTAACGCG
CAGACGCCAGGATTCAAATT

Product: flagellar biosynthesis regulator FlhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MGKHLVEKMEIFKANSKREIHKKIRLVTNEPYKITDERVTKLGIFKKQYEVTAVIMSEVAIADGRMDFQETFQKSVVKNR
PKTDDLLKKEKLLEMLAAGAELAQSTPLLEERKTQEEELSAMRLELAALNRELAVKMREEREQNSDFVKFLKGRGISDTY
VADFMQAGRKQFKQVETAHLDDITDWFVPYFSGKLAVEDSFDLSNHRIISLIGQTGVGKTTTLVKLGWQLLKQNRTVGFI
TTDTFRSGAVEQFQGYADKLDAELIVATSPAELEEAVQYMTYVNCVDHILIDTVGRNYLAEESVSEISAYTDVVHPDLTC
FTFSSGMKSADVMTILPKLAEIPIDGFIITKMDETTRIGDLYTVMQETNLPVLYMTDGQNITENIFRPKSRWLAERFVGT
DRRRVLE

Sequences:

>Translated_407_residues
MGKHLVEKMEIFKANSKREIHKKIRLVTNEPYKITDERVTKLGIFKKQYEVTAVIMSEVAIADGRMDFQETFQKSVVKNR
PKTDDLLKKEKLLEMLAAGAELAQSTPLLEERKTQEEELSAMRLELAALNRELAVKMREEREQNSDFVKFLKGRGISDTY
VADFMQAGRKQFKQVETAHLDDITDWFVPYFSGKLAVEDSFDLSNHRIISLIGQTGVGKTTTLVKLGWQLLKQNRTVGFI
TTDTFRSGAVEQFQGYADKLDAELIVATSPAELEEAVQYMTYVNCVDHILIDTVGRNYLAEESVSEISAYTDVVHPDLTC
FTFSSGMKSADVMTILPKLAEIPIDGFIITKMDETTRIGDLYTVMQETNLPVLYMTDGQNITENIFRPKSRWLAERFVGT
DRRRVLE
>Mature_406_residues
GKHLVEKMEIFKANSKREIHKKIRLVTNEPYKITDERVTKLGIFKKQYEVTAVIMSEVAIADGRMDFQETFQKSVVKNRP
KTDDLLKKEKLLEMLAAGAELAQSTPLLEERKTQEEELSAMRLELAALNRELAVKMREEREQNSDFVKFLKGRGISDTYV
ADFMQAGRKQFKQVETAHLDDITDWFVPYFSGKLAVEDSFDLSNHRIISLIGQTGVGKTTTLVKLGWQLLKQNRTVGFIT
TDTFRSGAVEQFQGYADKLDAELIVATSPAELEEAVQYMTYVNCVDHILIDTVGRNYLAEESVSEISAYTDVVHPDLTCF
TFSSGMKSADVMTILPKLAEIPIDGFIITKMDETTRIGDLYTVMQETNLPVLYMTDGQNITENIFRPKSRWLAERFVGTD
RRRVLE

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum [H]

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family [H]

Homologues:

Organism=Escherichia coli, GI1789874, Length=193, Percent_Identity=29.0155440414508, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788963, Length=199, Percent_Identity=26.1306532663317, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539958, Length=190, Percent_Identity=26.8421052631579, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325345, Length=187, Percent_Identity=28.8770053475936, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17647949, Length=279, Percent_Identity=23.6559139784946, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000897
- InterPro:   IPR020006 [H]

Pfam domain/function: PF00448 SRP54 [H]

EC number: NA

Molecular weight: Translated: 46387; Mature: 46256

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKHLVEKMEIFKANSKREIHKKIRLVTNEPYKITDERVTKLGIFKKQYEVTAVIMSEVA
CCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IADGRMDFQETFQKSVVKNRPKTDDLLKKEKLLEMLAAGAELAQSTPLLEERKTQEEELS
HHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHH
AMRLELAALNRELAVKMREEREQNSDFVKFLKGRGISDTYVADFMQAGRKQFKQVETAHL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DDITDWFVPYFSGKLAVEDSFDLSNHRIISLIGQTGVGKTTTLVKLGWQLLKQNRTVGFI
HHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
TTDTFRSGAVEQFQGYADKLDAELIVATSPAELEEAVQYMTYVNCVDHILIDTVGRNYLA
ECCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
EESVSEISAYTDVVHPDLTCFTFSSGMKSADVMTILPKLAEIPIDGFIITKMDETTRIGD
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
LYTVMQETNLPVLYMTDGQNITENIFRPKSRWLAERFVGTDRRRVLE
HHHHHHHCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
GKHLVEKMEIFKANSKREIHKKIRLVTNEPYKITDERVTKLGIFKKQYEVTAVIMSEVA
CHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IADGRMDFQETFQKSVVKNRPKTDDLLKKEKLLEMLAAGAELAQSTPLLEERKTQEEELS
HHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHH
AMRLELAALNRELAVKMREEREQNSDFVKFLKGRGISDTYVADFMQAGRKQFKQVETAHL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DDITDWFVPYFSGKLAVEDSFDLSNHRIISLIGQTGVGKTTTLVKLGWQLLKQNRTVGFI
HHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
TTDTFRSGAVEQFQGYADKLDAELIVATSPAELEEAVQYMTYVNCVDHILIDTVGRNYLA
ECCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
EESVSEISAYTDVVHPDLTCFTFSSGMKSADVMTILPKLAEIPIDGFIITKMDETTRIGD
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
LYTVMQETNLPVLYMTDGQNITENIFRPKSRWLAERFVGTDRRRVLE
HHHHHHHCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447978; 9384377 [H]