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The map label for this gene is fadH [C]

Identifier: 226223115

GI number: 226223115

Start: 522177

End: 524171

Strand: Direct

Name: fadH [C]

Synonym: Lm4b_00512

Alternate gene names: 226223115

Gene position: 522177-524171 (Clockwise)

Preceding gene: 226223113

Following gene: 226223116

Centisome position: 17.93

GC content: 41.75

Gene sequence:

>1995_bases
ATGACAGATTATCCGAGTATTTTTGAACCATTAACTGTAAAAAGAATGACCATTAAAAACCGTGTGATAATGCCACCAAT
GGGGACAAACCTAGCTGGATTAAATGGCGAATTTTTAGAAGAACATATGAATTACTACGAACAACGCGCAAAAGGTGGAA
CAGGTCTAATCACTATTGAAAACGCTTGTGTAGATTTTCCTTATGGGACAAACGGAACAACGCAACTTCGAATCGATAAT
GACCAATACATCCCTGGATTTTACAAATTAACAGAACGTCTTCATAAACATGGAACTTGTGTATCCATCCAAATCAACCA
CGCTGGTGCATCAGCTTATCCAGCACGTTTGAATGGACTTCAACCAGTTTCCGCGTCAGATATTCCATCTAAAAAAGGTG
GTACTGTACCGCGCCCGCTTACGGTAGAAGAAATTTATGAAATCGTCAATAAATATGGTGATGCAGCTAGACGTGCCCAA
CAAGCTGGGTTTGATGCAGTCGAAATCCACGGCGGACACTCGTACTTACTATGCCAATTCTTATCGCCACTTTATAACAA
ACGTACGGATGAATTTGGTGGCTCGCCTGAAAATCGCGCCCGTATCGTCAAACTAATTTTGGAAAAAGTCCGCGCGGAAG
TTGGTCCATTTTTCCCAATCGTACTTCGTTTTAGTGCAGATGAATTTACAGAAGGCGGCAACCACTTAGAAGATATTTTG
GAACTGCTAGATTATTGCCAAGAAGAAGCGGATATTTTGAATGTATCAGCGGCAATAAATGACAACCTATACTTGCAAAT
TGACCAAATGAACTTGGAAGATGGCTGGAGAAGCTACCTTGCAAAAGCGGTGAAAGATAAATTTAACAAACCAACGATTA
CTTCCGGTAACATTCGCAGTCCAAAAGCGGCAGAGAAAATTTTGTCAGAAGGATATGCGGACTTGCTTGCGATGGGACGT
GGCTTAATCGCTGAGCCTAACTGGGTGAACAAAGTGGCAACTGGCCAAGAAGACATGCTTCGAAAATGTATTTCTTGTAA
TATCGGTTGCGCGGATCACCGAATTTCCAAGTCGAAGCCAATCCGTTGTACGGTGAATCCTGATATTATTCATGAAGATA
AATACAAAGAAACAAAAGTGACTCGTCCGACCAATGTTGTCGTAATTGGCGGCGGAACAGCAGGGCTTGAAGCAGCTTGT
ACGGCGGCTGAAGTTGGCTGTAACACAACGCTAATTGAAGCGAGTGAACAAACTGGTGGCTTGGCGCGTGCCATTGCCAA
CCTTCCAGACAAAAGTAGAATTGCCGATTTCCCTAATTATTTAGCGAACCGAGCAGAAAAATTAACCAATTTAAAAGTTA
TTACAGGCACTAAAGCTGATACTGCGCTTATTGATACATTTAATCCTGATGTGGTAGTCAATGCCACAGGATCCAAACCA
TTACTTCCACCGATTAAAGGTTTGCATGACGTGATTGACAAAGAAGGCAGTAAGGTTCATTCGATTTTTGGACTTATTTC
AAATATCGATGACTTTACCGAATTTAGTAACAAAAAAGTTGCAGTTATCGGCGGTGGTGCAGTTGGACTTGATGTGGTCG
AGTACTTCTCAGAACGCGGCTCGGATGTTACAATTGTAGAAATGATGCCACTTCTTGGAAAAGACTTAGATATGATTACA
CGTCTTTCGATGATGGACATTATCGAGAAGAATAATGTTGATGTGCAAACAGAAACTGCATTAACCGAAGTAGCAGCGGA
TCATTTCAAAGTGAAGCACGACGGAGTAGACGCCGAGATTCCATTTGATTACGGCTTTGTCTGCCTAGGAATGCGACCAG
AACGCCCACTTATGGAAGAACTTGCGGCATACGGAAAAGAAAAACAAATTGAAATCGTAAATATTGGCGACAGCGCTGCG
ACAAGAAAAATCTTGGAAGGCGTTCGCGAAGGAAGAAATATTTTAACTACATTAGAAAAAATTGGCTCTTTGTAA

Upstream 100 bases:

>100_bases
TATTCCTGACTTATAATGAAAGAGAAATAAGATTGTGCATTATTGAACGTGAATAATGTAACAAATCATTTGCTGAGATT
ATAGTCGCAGGAGGCTTATT

Downstream 100 bases:

>100_bases
TCCGAACTGGCAAGGCGGAGTCCGTCTTGCCACATTCGCTCTTTATATAAGTTCACATTTTCACAAATAAACCTTAATTT
GGAAAGGAACGATGTTATTA

Product: NADH:flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MTDYPSIFEPLTVKRMTIKNRVIMPPMGTNLAGLNGEFLEEHMNYYEQRAKGGTGLITIENACVDFPYGTNGTTQLRIDN
DQYIPGFYKLTERLHKHGTCVSIQINHAGASAYPARLNGLQPVSASDIPSKKGGTVPRPLTVEEIYEIVNKYGDAARRAQ
QAGFDAVEIHGGHSYLLCQFLSPLYNKRTDEFGGSPENRARIVKLILEKVRAEVGPFFPIVLRFSADEFTEGGNHLEDIL
ELLDYCQEEADILNVSAAINDNLYLQIDQMNLEDGWRSYLAKAVKDKFNKPTITSGNIRSPKAAEKILSEGYADLLAMGR
GLIAEPNWVNKVATGQEDMLRKCISCNIGCADHRISKSKPIRCTVNPDIIHEDKYKETKVTRPTNVVVIGGGTAGLEAAC
TAAEVGCNTTLIEASEQTGGLARAIANLPDKSRIADFPNYLANRAEKLTNLKVITGTKADTALIDTFNPDVVVNATGSKP
LLPPIKGLHDVIDKEGSKVHSIFGLISNIDDFTEFSNKKVAVIGGGAVGLDVVEYFSERGSDVTIVEMMPLLGKDLDMIT
RLSMMDIIEKNNVDVQTETALTEVAADHFKVKHDGVDAEIPFDYGFVCLGMRPERPLMEELAAYGKEKQIEIVNIGDSAA
TRKILEGVREGRNILTTLEKIGSL

Sequences:

>Translated_664_residues
MTDYPSIFEPLTVKRMTIKNRVIMPPMGTNLAGLNGEFLEEHMNYYEQRAKGGTGLITIENACVDFPYGTNGTTQLRIDN
DQYIPGFYKLTERLHKHGTCVSIQINHAGASAYPARLNGLQPVSASDIPSKKGGTVPRPLTVEEIYEIVNKYGDAARRAQ
QAGFDAVEIHGGHSYLLCQFLSPLYNKRTDEFGGSPENRARIVKLILEKVRAEVGPFFPIVLRFSADEFTEGGNHLEDIL
ELLDYCQEEADILNVSAAINDNLYLQIDQMNLEDGWRSYLAKAVKDKFNKPTITSGNIRSPKAAEKILSEGYADLLAMGR
GLIAEPNWVNKVATGQEDMLRKCISCNIGCADHRISKSKPIRCTVNPDIIHEDKYKETKVTRPTNVVVIGGGTAGLEAAC
TAAEVGCNTTLIEASEQTGGLARAIANLPDKSRIADFPNYLANRAEKLTNLKVITGTKADTALIDTFNPDVVVNATGSKP
LLPPIKGLHDVIDKEGSKVHSIFGLISNIDDFTEFSNKKVAVIGGGAVGLDVVEYFSERGSDVTIVEMMPLLGKDLDMIT
RLSMMDIIEKNNVDVQTETALTEVAADHFKVKHDGVDAEIPFDYGFVCLGMRPERPLMEELAAYGKEKQIEIVNIGDSAA
TRKILEGVREGRNILTTLEKIGSL
>Mature_663_residues
TDYPSIFEPLTVKRMTIKNRVIMPPMGTNLAGLNGEFLEEHMNYYEQRAKGGTGLITIENACVDFPYGTNGTTQLRIDND
QYIPGFYKLTERLHKHGTCVSIQINHAGASAYPARLNGLQPVSASDIPSKKGGTVPRPLTVEEIYEIVNKYGDAARRAQQ
AGFDAVEIHGGHSYLLCQFLSPLYNKRTDEFGGSPENRARIVKLILEKVRAEVGPFFPIVLRFSADEFTEGGNHLEDILE
LLDYCQEEADILNVSAAINDNLYLQIDQMNLEDGWRSYLAKAVKDKFNKPTITSGNIRSPKAAEKILSEGYADLLAMGRG
LIAEPNWVNKVATGQEDMLRKCISCNIGCADHRISKSKPIRCTVNPDIIHEDKYKETKVTRPTNVVVIGGGTAGLEAACT
AAEVGCNTTLIEASEQTGGLARAIANLPDKSRIADFPNYLANRAEKLTNLKVITGTKADTALIDTFNPDVVVNATGSKPL
LPPIKGLHDVIDKEGSKVHSIFGLISNIDDFTEFSNKKVAVIGGGAVGLDVVEYFSERGSDVTIVEMMPLLGKDLDMITR
LSMMDIIEKNNVDVQTETALTEVAADHFKVKHDGVDAEIPFDYGFVCLGMRPERPLMEELAAYGKEKQIEIVNIGDSAAT
RKILEGVREGRNILTTLEKIGSL

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=543, Percent_Identity=30.0184162062615, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1787939, Length=368, Percent_Identity=30.1630434782609, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17559804, Length=309, Percent_Identity=29.4498381877023, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17564188, Length=309, Percent_Identity=29.126213592233, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=341, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17565138, Length=316, Percent_Identity=29.746835443038, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17540738, Length=342, Percent_Identity=26.0233918128655, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI72001454, Length=308, Percent_Identity=27.2727272727273, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17566914, Length=351, Percent_Identity=26.2108262108262, Blast_Score=106, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321973, Length=226, Percent_Identity=31.858407079646, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325086, Length=224, Percent_Identity=29.0178571428571, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 72815; Mature: 72684

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYPSIFEPLTVKRMTIKNRVIMPPMGTNLAGLNGEFLEEHMNYYEQRAKGGTGLITIE
CCCCCCHHCCHHHEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
NACVDFPYGTNGTTQLRIDNDQYIPGFYKLTERLHKHGTCVSIQINHAGASAYPARLNGL
CCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHCCCC
QPVSASDIPSKKGGTVPRPLTVEEIYEIVNKYGDAARRAQQAGFDAVEIHGGHSYLLCQF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
LSPLYNKRTDEFGGSPENRARIVKLILEKVRAEVGPFFPIVLRFSADEFTEGGNHLEDIL
HHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCCHHHHHHH
ELLDYCQEEADILNVSAAINDNLYLQIDQMNLEDGWRSYLAKAVKDKFNKPTITSGNIRS
HHHHHHHHHHHEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKAAEKILSEGYADLLAMGRGLIAEPNWVNKVATGQEDMLRKCISCNIGCADHRISKSKP
CHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCC
IRCTVNPDIIHEDKYKETKVTRPTNVVVIGGGTAGLEAACTAAEVGCNTTLIEASEQTGG
EEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCH
LARAIANLPDKSRIADFPNYLANRAEKLTNLKVITGTKADTALIDTFNPDVVVNATGSKP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCCEEEECCCCCC
LLPPIKGLHDVIDKEGSKVHSIFGLISNIDDFTEFSNKKVAVIGGGAVGLDVVEYFSERG
CCCHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCC
SDVTIVEMMPLLGKDLDMITRLSMMDIIEKNNVDVQTETALTEVAADHFKVKHDGVDAEI
CCEEHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHEEEECCCCCCCC
PFDYGFVCLGMRPERPLMEELAAYGKEKQIEIVNIGDSAATRKILEGVREGRNILTTLEK
CCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
IGSL
HCCC
>Mature Secondary Structure 
TDYPSIFEPLTVKRMTIKNRVIMPPMGTNLAGLNGEFLEEHMNYYEQRAKGGTGLITIE
CCCCCHHCCHHHEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
NACVDFPYGTNGTTQLRIDNDQYIPGFYKLTERLHKHGTCVSIQINHAGASAYPARLNGL
CCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHCCCC
QPVSASDIPSKKGGTVPRPLTVEEIYEIVNKYGDAARRAQQAGFDAVEIHGGHSYLLCQF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
LSPLYNKRTDEFGGSPENRARIVKLILEKVRAEVGPFFPIVLRFSADEFTEGGNHLEDIL
HHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCCHHHHHHH
ELLDYCQEEADILNVSAAINDNLYLQIDQMNLEDGWRSYLAKAVKDKFNKPTITSGNIRS
HHHHHHHHHHHEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKAAEKILSEGYADLLAMGRGLIAEPNWVNKVATGQEDMLRKCISCNIGCADHRISKSKP
CHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCC
IRCTVNPDIIHEDKYKETKVTRPTNVVVIGGGTAGLEAACTAAEVGCNTTLIEASEQTGG
EEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCH
LARAIANLPDKSRIADFPNYLANRAEKLTNLKVITGTKADTALIDTFNPDVVVNATGSKP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCCEEEECCCCCC
LLPPIKGLHDVIDKEGSKVHSIFGLISNIDDFTEFSNKKVAVIGGGAVGLDVVEYFSERG
CCCHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCC
SDVTIVEMMPLLGKDLDMITRLSMMDIIEKNNVDVQTETALTEVAADHFKVKHDGVDAEI
CCEEHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHEEEECCCCCCCC
PFDYGFVCLGMRPERPLMEELAAYGKEKQIEIVNIGDSAATRKILEGVREGRNILTTLEK
CCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
IGSL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]