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The map label for this gene is rlmN

Identifier: 226223105

GI number: 226223105

Start: 514805

End: 515908

Strand: Direct

Name: rlmN

Synonym: Lm4b_00502

Alternate gene names: 226223105

Gene position: 514805-515908 (Clockwise)

Preceding gene: 226223104

Following gene: 226223106

Centisome position: 17.67

GC content: 41.12

Gene sequence:

>1104_bases
ATGGAAAAAAGCTCCATTTATGGATTAACATGGACAAAATTAACAGAATGGCTAGAAGCACACGGTCAAAAGAAATTCCG
CGCAACACAAGTGTGGGACTGGCTTTATAGAAAACGTGTCAAAACTTTTGAAGAAATGAGTAACGTTCCAAAAGAAACAA
TTGAACTTTTAACAGCGAATTTTGTGATGAACACTTTAGAAGAACAAGTGGTACAAGAATCGACAGACGGCACGACGAAA
TATTTATTTAAGCTGAGTGACGGGAACTTAATTGAAACCGTGATGATGAAGCAAGAATATGGCTTGTCGGTTTGTGTAAC
GACTCAAGTTGGTTGTAATATCGGCTGTACTTTTTGTGCGAGTGGGCTTTTGAAAAAAAGTCGCGACTTAACTGCTGGCG
AAATCGTGGAACAAATTATGAATGTACAGCATTATTTGGATGGACGTAATTTGGAAGAACGCGTGAGTCACGTGGTTGTA
ATGGGAATCGGGGAACCGTTTGATAATTACGATAATGTGATGGATTTCTTGCGTGTGATTAATCATGATAAAGGTCTAGC
AATCGGCGCGCGCCATATCACTGTTTCGACAAGCGGCCTTGCACCGCGCATTATTGATTTTGCGAATGAAGATTTCCAAG
TCAACTTAGCGATTTCCCTCCATGCGCCGAACAATGAACTGCGGACGAGCATTATGCGTATTAACAAGACCTATTCAATT
GAGAAATTGATGGAAGCGATCCATTATTACGTTAACAAAACTAACCGCCGAATCACGTTTGAATACATTATGCTAAAAGG
TGTAAACGACCATAAAAAAGAAGCGCTCGAACTGGCGGCACTTCTCGGAGAACATCGCCATTTAGCTTATGTTAACTTGA
TTCCTTACAACCCGGTGGATGAGCATATCGATTATGAACGTAGCACAAAAGAAGACGTACTCGCTTTCTATGATACATTG
AAGAAAAATGGTATTAATTGTGTTATTCGCCGCGAACACGGGACAGATATTGATGCCGCGTGTGGGCAACTTCGTAGTAA
ACAAATCAAACGAGTTGGCGTGCGCGAACGGATGAAACAAAAACAAGCAGCAGCGGAAGAATAA

Upstream 100 bases:

>100_bases
CAGGACAAACGGCTGATATTTACGAAATAGCATTGCTTGTGAACCATAAAACAGCTATAATCTTTATAGACGAAAACAAG
ACCTGAAAAGAGGCTGAACA

Downstream 100 bases:

>100_bases
TAATTCAAACTATCTGGTTTCCATTTGGAGATTGGATAGTTTTTGTTTATACTTAAAGTACCGAAAGCAATTGAAGGAGG
ACAAAAATCATGGAAGTAGA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MEKSSIYGLTWTKLTEWLEAHGQKKFRATQVWDWLYRKRVKTFEEMSNVPKETIELLTANFVMNTLEEQVVQESTDGTTK
YLFKLSDGNLIETVMMKQEYGLSVCVTTQVGCNIGCTFCASGLLKKSRDLTAGEIVEQIMNVQHYLDGRNLEERVSHVVV
MGIGEPFDNYDNVMDFLRVINHDKGLAIGARHITVSTSGLAPRIIDFANEDFQVNLAISLHAPNNELRTSIMRINKTYSI
EKLMEAIHYYVNKTNRRITFEYIMLKGVNDHKKEALELAALLGEHRHLAYVNLIPYNPVDEHIDYERSTKEDVLAFYDTL
KKNGINCVIRREHGTDIDAACGQLRSKQIKRVGVRERMKQKQAAAEE

Sequences:

>Translated_367_residues
MEKSSIYGLTWTKLTEWLEAHGQKKFRATQVWDWLYRKRVKTFEEMSNVPKETIELLTANFVMNTLEEQVVQESTDGTTK
YLFKLSDGNLIETVMMKQEYGLSVCVTTQVGCNIGCTFCASGLLKKSRDLTAGEIVEQIMNVQHYLDGRNLEERVSHVVV
MGIGEPFDNYDNVMDFLRVINHDKGLAIGARHITVSTSGLAPRIIDFANEDFQVNLAISLHAPNNELRTSIMRINKTYSI
EKLMEAIHYYVNKTNRRITFEYIMLKGVNDHKKEALELAALLGEHRHLAYVNLIPYNPVDEHIDYERSTKEDVLAFYDTL
KKNGINCVIRREHGTDIDAACGQLRSKQIKRVGVRERMKQKQAAAEE
>Mature_367_residues
MEKSSIYGLTWTKLTEWLEAHGQKKFRATQVWDWLYRKRVKTFEEMSNVPKETIELLTANFVMNTLEEQVVQESTDGTTK
YLFKLSDGNLIETVMMKQEYGLSVCVTTQVGCNIGCTFCASGLLKKSRDLTAGEIVEQIMNVQHYLDGRNLEERVSHVVV
MGIGEPFDNYDNVMDFLRVINHDKGLAIGARHITVSTSGLAPRIIDFANEDFQVNLAISLHAPNNELRTSIMRINKTYSI
EKLMEAIHYYVNKTNRRITFEYIMLKGVNDHKKEALELAALLGEHRHLAYVNLIPYNPVDEHIDYERSTKEDVLAFYDTL
KKNGINCVIRREHGTDIDAACGQLRSKQIKRVGVRERMKQKQAAAEE

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=364, Percent_Identity=34.8901098901099, Blast_Score=214, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_LISIN (Q92EH6)

Other databases:

- EMBL:   AL596165
- PIR:   AD1493
- RefSeq:   NP_469827.1
- ProteinModelPortal:   Q92EH6
- GeneID:   1128934
- GenomeReviews:   AL592022_GR
- KEGG:   lin:lin0484
- GenoList:   LIN0484
- HOGENOM:   HBG335345
- OMA:   VQKYFDE
- ProtClustDB:   PRK14455
- BioCyc:   LINN272626:LIN0484-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 42102; Mature: 42102

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSSIYGLTWTKLTEWLEAHGQKKFRATQVWDWLYRKRVKTFEEMSNVPKETIELLTAN
CCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FVMNTLEEQVVQESTDGTTKYLFKLSDGNLIETVMMKQEYGLSVCVTTQVGCNIGCTFCA
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
SGLLKKSRDLTAGEIVEQIMNVQHYLDGRNLEERVSHVVVMGIGEPFDNYDNVMDFLRVI
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCCHHHHHHHHHHH
NHDKGLAIGARHITVSTSGLAPRIIDFANEDFQVNLAISLHAPNNELRTSIMRINKTYSI
CCCCCCEEEEEEEEEECCCCCHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCH
EKLMEAIHYYVNKTNRRITFEYIMLKGVNDHKKEALELAALLGEHRHLAYVNLIPYNPVD
HHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
EHIDYERSTKEDVLAFYDTLKKNGINCVIRREHGTDIDAACGQLRSKQIKRVGVRERMKQ
HHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQAAAEE
HHHCCCC
>Mature Secondary Structure
MEKSSIYGLTWTKLTEWLEAHGQKKFRATQVWDWLYRKRVKTFEEMSNVPKETIELLTAN
CCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FVMNTLEEQVVQESTDGTTKYLFKLSDGNLIETVMMKQEYGLSVCVTTQVGCNIGCTFCA
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
SGLLKKSRDLTAGEIVEQIMNVQHYLDGRNLEERVSHVVVMGIGEPFDNYDNVMDFLRVI
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCCHHHHHHHHHHH
NHDKGLAIGARHITVSTSGLAPRIIDFANEDFQVNLAISLHAPNNELRTSIMRINKTYSI
CCCCCCEEEEEEEEEECCCCCHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCH
EKLMEAIHYYVNKTNRRITFEYIMLKGVNDHKKEALELAALLGEHRHLAYVNLIPYNPVD
HHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
EHIDYERSTKEDVLAFYDTLKKNGINCVIRREHGTDIDAACGQLRSKQIKRVGVRERMKQ
HHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQAAAEE
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669