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The map label for this gene is pps [H]

Identifier: 226223040

GI number: 226223040

Start: 439090

End: 441693

Strand: Reverse

Name: pps [H]

Synonym: Lm4b_00434

Alternate gene names: 226223040

Gene position: 441693-439090 (Counterclockwise)

Preceding gene: 226223041

Following gene: 226223020

Centisome position: 15.16

GC content: 41.9

Gene sequence:

>2604_bases
ATGAAACCATATGTACTCAAGTTTCAAGAAATACGACCGCATTCAGAAGCACTTGTCGGTGGTAAAGGAATGAATTTAGG
TGCTTGCTCTTATATCGAAGGAGTACATGTTCCCGCAGGTTTTTGCCTGACAACAGAAGCTTACAAGCGAACCTTAGCGG
AAAACAATGAATTTACGCAACTGCTCCAACGCCTTTCTTCCCTAAAAACAAGTGACATGGATGCTATTAGAGAAATCAGC
GAAACGATTCGAACGCTTATTCAACATACACAAATCCCCTCTGAAATAGCGAGTTATATGGATGCTACGCTTTTAGATGT
GGGTGGGTATGAGATGCCTTTCGCCGTACGTTCTAGCGCCACTGCCGAAGACCTTCCCCACGCTTCCTTCGCTGGGCAAC
ATGATACGTATTTAAATATTATTGGTAAAGATGCCTTATTACAACATATCAGTATGTGCTGGGCTTCTTTGTTTACCGAA
CGCGCGATTATTTATCGCATTCAAAATCAGTTCGATCATCGTAAAGTCCAACTTGCCGTCGTTATTCAACAAATGATTTC
CCCCGAAGCATCCGGAATCCTATTTACGGCTGATCCAATTACATCGAATCGCAAATCCCTCTCTATCGATGCCAGTTTCG
GGCTAGGTGAAGCGCTTGTTTCCGGTTTAGTTTCTGCTGACTCATACACAGTCCGAGAAAATACTATTACGAACAAAATA
ATCGCTACTAAAAAACTCGCCATTTACAGTTTAAAAGAAGGTGGAACGGAGACTCGCATACTTGAAAAATCACAACAAAC
CAAGCAAACATTAACTGACCAACAAATAATACAACTTGCGAAACTTGGTAGAAAAATCGAAGCCTATTTTGGGAAACCAC
AAGATATTGAATGGTGTTTAGCGGAAGGTGCTTTTTACATTGTTCAGAGTCGACCTATCACGACTTTATATCCAATTCCA
GAAGTAAACGAACCCGGCAATCGTGTTTATATTTCTGTTGCACATCAGCAAATGATGACTGACGCGATGAAACCACTGGG
ACTTTCCTTTTATTTAATGACGACTCCCGCCACGATGTATACAGCAGGAGGGCGGTTGTTTGTCGATATTACTCAGAGCC
TGTCAGCCAAAGTTTCTCGCGATATGATGGTCAATTCACTCGGTCAATCAGACCCACTTATTAAAGATGCCTTGCTCACT
GTAATTAACAAAAAAGGATTCCTGCCTCCACTACCAACCGAGGAAAATCCAAGTCAGGCACCCGTGTCTGGAAAACCACC
CGTGCGTAGCATTCCTGATTCATCTAGCGTTTTTGAATTAGTTCGAAACAGCGAAAACTCTATAAAGCACTTGAAGCAAT
CCATTGAAACGAAAAACGGATCTGATTTGTTTGATTTTATCGTAGAGGATTTGGAAGAATTAAAGCGAGTTTTATTTAAT
CCTACTAGCATTGATGCCATTATGGCAGGTATGGACGCTTCAGCTTGGTTAAATGAACATATTTACCAGTGGCTCGGAGA
AAAAAATGTCGCCGATAAACTGTCAGAATCAGCCCCAAACAATATTACTTCACAAATGGGACTTGAATTATTAGATGTAG
CTGACGTCATTCGCCCCTACCCTGCTGTCAGAGCCTATTTAGAACAAACGAAAAACCCTGATTTCATGAATGAACTAGCT
ACTTTAGAAGGTGGTGCAGAAACCAAAAAAGCGCTAGAAGATTACCTTCAAAAATACGGGATGCGCTGTGCGGGTGAAAT
TGACTTAACCAAAACTCGTTGGATTGAAAACCCGCTAACGCTTATTCCGCTGATTTTAAGTAACATCAAAAATTTCGACT
CCGGCGCGAGTATGCACAAATTTGCTCAGGGAGAAAAAGAAGCTTTCCATAAAGAACAAGAAATCCTAAGACGTTTGCAA
GAGCTTCCGGACGGGGAACAAAAAGCGATGGAAACAAAAGAAAAAATCGATATTTTACGTCATTTCATCGGCTATCGTGA
GTATCCTAAATACGGGATGATTAATCGCTACTTTATTTACAAACTGGCTTTACTTCGAGCTGGCGAACAACTTGTAAAAG
ATGGCATTCTGCAAGAGCAAGAAGATATTTATTTTCTTTACTTTGAGGAACTGCGCGAAGTCGTGCGTACTGGTCAAGTT
GATTACGAACTTATTAACGTTCGAAAACGTGATTTCGCCACTTTTGAAAAATTAACGCCACCACGCATCCTTACTTCTGA
TGGAGAAATGATTAACGGCGAATACAAACGCGAAAATCTCCCTAAAGACGCCATTCTTGGACTTCCAGTTTCATCAGGGA
CAGTTGAAGGTCGCGCTCGCGTCATTTTAGATATGGAAAAAGCGGATTTAGAAGATGGCGATATTCTTGTTACGGCTTAT
ACTGACCCTAGTTGGACTCCCGCATTTGTATCTATTAAAGGGCTTGTGACCGAGGTTGGCGGGCTTATGACGCACGGAGC
AGTGATTGCGCGCGAATACGGCCTTCCCGCTGTCGTGGGTGTCGAAAATGCAACTACCATTATAAAAGATGGCCAGCAAA
TTCGCATCAATGGGACAGAAGGCTATATTGAGATTCTTGATTAA

Upstream 100 bases:

>100_bases
GTAAAATATACTAATGTAAAGCAATCCCTACTTTAATTATACAACAAGAGGTTCTTTACAGTCAAGTATTATTTTTCGAA
AAAAATTGGGGGATAAATAT

Downstream 100 bases:

>100_bases
ACCAAAAACCCAGTAATCTCCATTTTAGGAAATACTGGGTTTTTTAATTATGCTTTTTTTCTCCAAATATAAAACGCGGA
TGATGAAAGTAATATCCCGG

Product: phosphoenolpyruvate synthase

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 867; Mature: 867

Protein sequence:

>867_residues
MKPYVLKFQEIRPHSEALVGGKGMNLGACSYIEGVHVPAGFCLTTEAYKRTLAENNEFTQLLQRLSSLKTSDMDAIREIS
ETIRTLIQHTQIPSEIASYMDATLLDVGGYEMPFAVRSSATAEDLPHASFAGQHDTYLNIIGKDALLQHISMCWASLFTE
RAIIYRIQNQFDHRKVQLAVVIQQMISPEASGILFTADPITSNRKSLSIDASFGLGEALVSGLVSADSYTVRENTITNKI
IATKKLAIYSLKEGGTETRILEKSQQTKQTLTDQQIIQLAKLGRKIEAYFGKPQDIEWCLAEGAFYIVQSRPITTLYPIP
EVNEPGNRVYISVAHQQMMTDAMKPLGLSFYLMTTPATMYTAGGRLFVDITQSLSAKVSRDMMVNSLGQSDPLIKDALLT
VINKKGFLPPLPTEENPSQAPVSGKPPVRSIPDSSSVFELVRNSENSIKHLKQSIETKNGSDLFDFIVEDLEELKRVLFN
PTSIDAIMAGMDASAWLNEHIYQWLGEKNVADKLSESAPNNITSQMGLELLDVADVIRPYPAVRAYLEQTKNPDFMNELA
TLEGGAETKKALEDYLQKYGMRCAGEIDLTKTRWIENPLTLIPLILSNIKNFDSGASMHKFAQGEKEAFHKEQEILRRLQ
ELPDGEQKAMETKEKIDILRHFIGYREYPKYGMINRYFIYKLALLRAGEQLVKDGILQEQEDIYFLYFEELREVVRTGQV
DYELINVRKRDFATFEKLTPPRILTSDGEMINGEYKRENLPKDAILGLPVSSGTVEGRARVILDMEKADLEDGDILVTAY
TDPSWTPAFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATTIIKDGQQIRINGTEGYIEILD

Sequences:

>Translated_867_residues
MKPYVLKFQEIRPHSEALVGGKGMNLGACSYIEGVHVPAGFCLTTEAYKRTLAENNEFTQLLQRLSSLKTSDMDAIREIS
ETIRTLIQHTQIPSEIASYMDATLLDVGGYEMPFAVRSSATAEDLPHASFAGQHDTYLNIIGKDALLQHISMCWASLFTE
RAIIYRIQNQFDHRKVQLAVVIQQMISPEASGILFTADPITSNRKSLSIDASFGLGEALVSGLVSADSYTVRENTITNKI
IATKKLAIYSLKEGGTETRILEKSQQTKQTLTDQQIIQLAKLGRKIEAYFGKPQDIEWCLAEGAFYIVQSRPITTLYPIP
EVNEPGNRVYISVAHQQMMTDAMKPLGLSFYLMTTPATMYTAGGRLFVDITQSLSAKVSRDMMVNSLGQSDPLIKDALLT
VINKKGFLPPLPTEENPSQAPVSGKPPVRSIPDSSSVFELVRNSENSIKHLKQSIETKNGSDLFDFIVEDLEELKRVLFN
PTSIDAIMAGMDASAWLNEHIYQWLGEKNVADKLSESAPNNITSQMGLELLDVADVIRPYPAVRAYLEQTKNPDFMNELA
TLEGGAETKKALEDYLQKYGMRCAGEIDLTKTRWIENPLTLIPLILSNIKNFDSGASMHKFAQGEKEAFHKEQEILRRLQ
ELPDGEQKAMETKEKIDILRHFIGYREYPKYGMINRYFIYKLALLRAGEQLVKDGILQEQEDIYFLYFEELREVVRTGQV
DYELINVRKRDFATFEKLTPPRILTSDGEMINGEYKRENLPKDAILGLPVSSGTVEGRARVILDMEKADLEDGDILVTAY
TDPSWTPAFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATTIIKDGQQIRINGTEGYIEILD
>Mature_867_residues
MKPYVLKFQEIRPHSEALVGGKGMNLGACSYIEGVHVPAGFCLTTEAYKRTLAENNEFTQLLQRLSSLKTSDMDAIREIS
ETIRTLIQHTQIPSEIASYMDATLLDVGGYEMPFAVRSSATAEDLPHASFAGQHDTYLNIIGKDALLQHISMCWASLFTE
RAIIYRIQNQFDHRKVQLAVVIQQMISPEASGILFTADPITSNRKSLSIDASFGLGEALVSGLVSADSYTVRENTITNKI
IATKKLAIYSLKEGGTETRILEKSQQTKQTLTDQQIIQLAKLGRKIEAYFGKPQDIEWCLAEGAFYIVQSRPITTLYPIP
EVNEPGNRVYISVAHQQMMTDAMKPLGLSFYLMTTPATMYTAGGRLFVDITQSLSAKVSRDMMVNSLGQSDPLIKDALLT
VINKKGFLPPLPTEENPSQAPVSGKPPVRSIPDSSSVFELVRNSENSIKHLKQSIETKNGSDLFDFIVEDLEELKRVLFN
PTSIDAIMAGMDASAWLNEHIYQWLGEKNVADKLSESAPNNITSQMGLELLDVADVIRPYPAVRAYLEQTKNPDFMNELA
TLEGGAETKKALEDYLQKYGMRCAGEIDLTKTRWIENPLTLIPLILSNIKNFDSGASMHKFAQGEKEAFHKEQEILRRLQ
ELPDGEQKAMETKEKIDILRHFIGYREYPKYGMINRYFIYKLALLRAGEQLVKDGILQEQEDIYFLYFEELREVVRTGQV
DYELINVRKRDFATFEKLTPPRILTSDGEMINGEYKRENLPKDAILGLPVSSGTVEGRARVILDMEKADLEDGDILVTAY
TDPSWTPAFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATTIIKDGQQIRINGTEGYIEILD

Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=333, Percent_Identity=33.033033033033, Blast_Score=171, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17564524, Length=313, Percent_Identity=31.3099041533546, Blast_Score=132, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 96924; Mature: 96924

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYVLKFQEIRPHSEALVGGKGMNLGACSYIEGVHVPAGFCLTTEAYKRTLAENNEFTQ
CCCCEEEHHHCCCCHHHHCCCCCCCCCCHHHHCCCCCCCCEEEEHHHHHHHHHCCCHHHH
LLQRLSSLKTSDMDAIREISETIRTLIQHTQIPSEIASYMDATLLDVGGYEMPFAVRSSA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHCCCC
TAEDLPHASFAGQHDTYLNIIGKDALLQHISMCWASLFTERAIIYRIQNQFDHRKVQLAV
CHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VIQQMISPEASGILFTADPITSNRKSLSIDASFGLGEALVSGLVSADSYTVRENTITNKI
HHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHH
IATKKLAIYSLKEGGTETRILEKSQQTKQTLTDQQIIQLAKLGRKIEAYFGKPQDIEWCL
HHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHH
AEGAFYIVQSRPITTLYPIPEVNEPGNRVYISVAHQQMMTDAMKPLGLSFYLMTTPATMY
HCCEEEEEECCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCCCEEEEEEECCCCEE
TAGGRLFVDITQSLSAKVSRDMMVNSLGQSDPLIKDALLTVINKKGFLPPLPTEENPSQA
ECCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PVSGKPPVRSIPDSSSVFELVRNSENSIKHLKQSIETKNGSDLFDFIVEDLEELKRVLFN
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
PTSIDAIMAGMDASAWLNEHIYQWLGEKNVADKLSESAPNNITSQMGLELLDVADVIRPY
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHCHHHHHHHHHHCCC
PAVRAYLEQTKNPDFMNELATLEGGAETKKALEDYLQKYGMRCAGEIDLTKTRWIENPLT
HHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHH
LIPLILSNIKNFDSGASMHKFAQGEKEAFHKEQEILRRLQELPDGEQKAMETKEKIDILR
HHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
HFIGYREYPKYGMINRYFIYKLALLRAGEQLVKDGILQEQEDIYFLYFEELREVVRTGQV
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCC
DYELINVRKRDFATFEKLTPPRILTSDGEMINGEYKRENLPKDAILGLPVSSGTVEGRAR
CHHHHHHHHHHHHHHHHCCCCCEECCCCCEECCCHHHCCCCCHHEEECCCCCCCCCCCEE
VILDMEKADLEDGDILVTAYTDPSWTPAFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVG
EEEEEHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEEEE
VENATTIIKDGQQIRINGTEGYIEILD
CCCCHHEEECCCEEEEECCCCEEEECC
>Mature Secondary Structure
MKPYVLKFQEIRPHSEALVGGKGMNLGACSYIEGVHVPAGFCLTTEAYKRTLAENNEFTQ
CCCCEEEHHHCCCCHHHHCCCCCCCCCCHHHHCCCCCCCCEEEEHHHHHHHHHCCCHHHH
LLQRLSSLKTSDMDAIREISETIRTLIQHTQIPSEIASYMDATLLDVGGYEMPFAVRSSA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHCCCC
TAEDLPHASFAGQHDTYLNIIGKDALLQHISMCWASLFTERAIIYRIQNQFDHRKVQLAV
CHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VIQQMISPEASGILFTADPITSNRKSLSIDASFGLGEALVSGLVSADSYTVRENTITNKI
HHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHH
IATKKLAIYSLKEGGTETRILEKSQQTKQTLTDQQIIQLAKLGRKIEAYFGKPQDIEWCL
HHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHH
AEGAFYIVQSRPITTLYPIPEVNEPGNRVYISVAHQQMMTDAMKPLGLSFYLMTTPATMY
HCCEEEEEECCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCCCEEEEEEECCCCEE
TAGGRLFVDITQSLSAKVSRDMMVNSLGQSDPLIKDALLTVINKKGFLPPLPTEENPSQA
ECCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PVSGKPPVRSIPDSSSVFELVRNSENSIKHLKQSIETKNGSDLFDFIVEDLEELKRVLFN
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
PTSIDAIMAGMDASAWLNEHIYQWLGEKNVADKLSESAPNNITSQMGLELLDVADVIRPY
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHCHHHHHHHHHHCCC
PAVRAYLEQTKNPDFMNELATLEGGAETKKALEDYLQKYGMRCAGEIDLTKTRWIENPLT
HHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHH
LIPLILSNIKNFDSGASMHKFAQGEKEAFHKEQEILRRLQELPDGEQKAMETKEKIDILR
HHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
HFIGYREYPKYGMINRYFIYKLALLRAGEQLVKDGILQEQEDIYFLYFEELREVVRTGQV
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCC
DYELINVRKRDFATFEKLTPPRILTSDGEMINGEYKRENLPKDAILGLPVSSGTVEGRAR
CHHHHHHHHHHHHHHHHCCCCCEECCCCCEECCCHHHCCCCCHHEEECCCCCCCCCCCEE
VILDMEKADLEDGDILVTAYTDPSWTPAFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVG
EEEEEHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEEEE
VENATTIIKDGQQIRINGTEGYIEILD
CCCCHHEEECCCEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.083 {pyruvate}} 10.5 {phosphate}} 0.028 {ATP}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7704256 [H]