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The map label for this gene is lacG [H]

Identifier: 226222936

GI number: 226222936

Start: 333491

End: 334882

Strand: Reverse

Name: lacG [H]

Synonym: Lm4b_00328

Alternate gene names: 226222936

Gene position: 334882-333491 (Counterclockwise)

Preceding gene: 226222937

Following gene: 226222935

Centisome position: 11.5

GC content: 43.25

Gene sequence:

>1392_bases
ATGAATAACAACTTTTTATGGGGAGGTGCAACGGCCTCTTATCAATGTGAAGGTGCTTGGGATGTGGATGGCAAGGCGGA
ATCGATGTGGGACTACTACTTGCACGAAGCAGGTCTTGAGAATGGCGATGTGGCGAGCGATCACTACCACCGCTACGAGG
AAGACATTCGGATGATGAAAGAAGGCGGGCAAAATTCCTATCGTTTTTCCTTATCTTGGCCGCGAATTATTAAAAATAGG
CAAGGTGACATCAACTTAAAAGGGATCGAATTTTATCAAAACTTGCTAGATACGTGTAAAAAATATGATATTGAGCCGTT
TGTGACACTTTATCATTGGGATTTGCCGCAATATTGGGAAGAAACTGGTGGCTGGTTGGATCACGACGTTTGTGCTGCAT
TCGAGCATTATGCCAAGGTTTGTTATGACCACTTTGGCGATAAAATCACGAATTGGACTACGTTTAACGAGCCAAAATGG
TTTGTTGCTAATGGATATAAAATCGGCAACTACCCGCCCGGCTATCAAGATACACAAAAAACGATGATTGCCGCTTATAA
TGTGATGTACGCGAGTGCTTTAGGCGTCAAAGCTTTTAAAGAAGGTGGCTACTCAGGGCAAATTGGTATTGTTCATAGCT
ACACGCCGGTGAACGGTGTCGATGAAAGTATCGAAACTAAAATCGCGATGCGCTACGCTGATAACTATTGCAACAACTGG
ATTCTCGACACAGCCGCACTTGGCGAATTCCCGGTCGACCTCATTGCCGAACTAGCAAAATCGCACGACATCAGCTTTAT
GAAAACAGACGAACTACAAACAATTAAACAAAACACTGTCGACTTCATCGGCTTAAATTACTATTCGCGCACGCTCGTTA
AACCATACACTGGCGGCGAAACCCAACTACAATTCAACCACAGCGGTAAAAAAGGCGAAAGTAAAGTTCTCATCAAAAAC
TGGTTCGAACAAGTGAAAGATCCCGCCAACGAAACAACCGAATGGGACACAGAAATTTATCCAAAAGGCTTACAAGATGG
CTTAATCGAGGCTTACGAACGCTATCAATTACCACTTTATGTGACAGAAAACGGCATTGGTGTTCGCGAAGATGTTTCAG
TGGCAAAAGTAGATGATGCCTACCGAATCGCCTTTATGAACGACCATATTAACGCCATTTTCAACGCAATCGATGCTGGT
TGTGACGTTCGTGGCTACTACGCTTGGTCCCCGTTCGACCTTTACTCTTGGAAAAATGGTGTCGAAAAGCGTTACGGACT
TGTAGCAGTTGATTTCGAAAACAACCAAATCAGAAAACCAAAAGCTTCCTACTACTGGTTCAAAGAAATGATTGAAAGTC
AGGGAAAACTCATCAAACGAAGAGAATTTTAA

Upstream 100 bases:

>100_bases
CTGCTTGTTCATGCGGAAGGTCACTTGTCTTCTACGGATATTGCCGTGGAAATGATTCGGGAACTGGGAGAAATTTATCA
ATGGAAATGGAGTTTATCTA

Downstream 100 bases:

>100_bases
AAAAAGGAGCAGGAAAATGAAAAATATCATGTTAGTTTGTAATGCTGGTATGTCTACAGGAATGCTTGCGAAAAAAATCG
AAGCCGCTTCTGGCAACACA

Product: phospho-beta-glucosidase and phospho-beta-galactosidase

Products: NA

Alternate protein names: Beta-D-phosphogalactoside galactohydrolase; PGALase; P-beta-Gal; PBG [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNNNFLWGGATASYQCEGAWDVDGKAESMWDYYLHEAGLENGDVASDHYHRYEEDIRMMKEGGQNSYRFSLSWPRIIKNR
QGDINLKGIEFYQNLLDTCKKYDIEPFVTLYHWDLPQYWEETGGWLDHDVCAAFEHYAKVCYDHFGDKITNWTTFNEPKW
FVANGYKIGNYPPGYQDTQKTMIAAYNVMYASALGVKAFKEGGYSGQIGIVHSYTPVNGVDESIETKIAMRYADNYCNNW
ILDTAALGEFPVDLIAELAKSHDISFMKTDELQTIKQNTVDFIGLNYYSRTLVKPYTGGETQLQFNHSGKKGESKVLIKN
WFEQVKDPANETTEWDTEIYPKGLQDGLIEAYERYQLPLYVTENGIGVREDVSVAKVDDAYRIAFMNDHINAIFNAIDAG
CDVRGYYAWSPFDLYSWKNGVEKRYGLVAVDFENNQIRKPKASYYWFKEMIESQGKLIKRREF

Sequences:

>Translated_463_residues
MNNNFLWGGATASYQCEGAWDVDGKAESMWDYYLHEAGLENGDVASDHYHRYEEDIRMMKEGGQNSYRFSLSWPRIIKNR
QGDINLKGIEFYQNLLDTCKKYDIEPFVTLYHWDLPQYWEETGGWLDHDVCAAFEHYAKVCYDHFGDKITNWTTFNEPKW
FVANGYKIGNYPPGYQDTQKTMIAAYNVMYASALGVKAFKEGGYSGQIGIVHSYTPVNGVDESIETKIAMRYADNYCNNW
ILDTAALGEFPVDLIAELAKSHDISFMKTDELQTIKQNTVDFIGLNYYSRTLVKPYTGGETQLQFNHSGKKGESKVLIKN
WFEQVKDPANETTEWDTEIYPKGLQDGLIEAYERYQLPLYVTENGIGVREDVSVAKVDDAYRIAFMNDHINAIFNAIDAG
CDVRGYYAWSPFDLYSWKNGVEKRYGLVAVDFENNQIRKPKASYYWFKEMIESQGKLIKRREF
>Mature_463_residues
MNNNFLWGGATASYQCEGAWDVDGKAESMWDYYLHEAGLENGDVASDHYHRYEEDIRMMKEGGQNSYRFSLSWPRIIKNR
QGDINLKGIEFYQNLLDTCKKYDIEPFVTLYHWDLPQYWEETGGWLDHDVCAAFEHYAKVCYDHFGDKITNWTTFNEPKW
FVANGYKIGNYPPGYQDTQKTMIAAYNVMYASALGVKAFKEGGYSGQIGIVHSYTPVNGVDESIETKIAMRYADNYCNNW
ILDTAALGEFPVDLIAELAKSHDISFMKTDELQTIKQNTVDFIGLNYYSRTLVKPYTGGETQLQFNHSGKKGESKVLIKN
WFEQVKDPANETTEWDTEIYPKGLQDGLIEAYERYQLPLYVTENGIGVREDVSVAKVDDAYRIAFMNDHINAIFNAIDAG
CDVRGYYAWSPFDLYSWKNGVEKRYGLVAVDFENNQIRKPKASYYWFKEMIESQGKLIKRREF

Specific function: Can Hydrolyze Salicin And Arbutin. [C]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=477, Percent_Identity=32.0754716981132, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI32481206, Length=489, Percent_Identity=31.2883435582822, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI13273313, Length=473, Percent_Identity=31.0782241014799, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI24497614, Length=489, Percent_Identity=28.0163599182004, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI28376633, Length=189, Percent_Identity=37.037037037037, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI190360571, Length=93, Percent_Identity=44.0860215053763, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI2367270, Length=485, Percent_Identity=32.1649484536082, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1789070, Length=486, Percent_Identity=30.2469135802469, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI2367174, Length=495, Percent_Identity=29.4949494949495, Blast_Score=188, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17552856, Length=482, Percent_Identity=31.5352697095436, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17539390, Length=479, Percent_Identity=27.9749478079332, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI21356577, Length=479, Percent_Identity=32.5678496868476, Blast_Score=238, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005928 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.85 [H]

Molecular weight: Translated: 53551; Mature: 53551

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNFLWGGATASYQCEGAWDVDGKAESMWDYYLHEAGLENGDVASDHYHRYEEDIRMMK
CCCCEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
EGGQNSYRFSLSWPRIIKNRQGDINLKGIEFYQNLLDTCKKYDIEPFVTLYHWDLPQYWE
HCCCCCEEEEEECHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
ETGGWLDHDVCAAFEHYAKVCYDHFGDKITNWTTFNEPKWFVANGYKIGNYPPGYQDTQK
HHCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCEECCCCCEEEECCEECCCCCCCCCHHHH
TMIAAYNVMYASALGVKAFKEGGYSGQIGIVHSYTPVNGVDESIETKIAMRYADNYCNNW
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
ILDTAALGEFPVDLIAELAKSHDISFMKTDELQTIKQNTVDFIGLNYYSRTLVKPYTGGE
EEEHHHCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHCCCEEEEECHHHHHEECCCCCCC
TQLQFNHSGKKGESKVLIKNWFEQVKDPANETTEWDTEIYPKGLQDGLIEAYERYQLPLY
EEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEE
VTENGIGVREDVSVAKVDDAYRIAFMNDHINAIFNAIDAGCDVRGYYAWSPFDLYSWKNG
EECCCCCCCCCCCEEEECCCEEEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCC
VEKRYGLVAVDFENNQIRKPKASYYWFKEMIESQGKLIKRREF
HHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure
MNNNFLWGGATASYQCEGAWDVDGKAESMWDYYLHEAGLENGDVASDHYHRYEEDIRMMK
CCCCEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
EGGQNSYRFSLSWPRIIKNRQGDINLKGIEFYQNLLDTCKKYDIEPFVTLYHWDLPQYWE
HCCCCCEEEEEECHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
ETGGWLDHDVCAAFEHYAKVCYDHFGDKITNWTTFNEPKWFVANGYKIGNYPPGYQDTQK
HHCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCEECCCCCEEEECCEECCCCCCCCCHHHH
TMIAAYNVMYASALGVKAFKEGGYSGQIGIVHSYTPVNGVDESIETKIAMRYADNYCNNW
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
ILDTAALGEFPVDLIAELAKSHDISFMKTDELQTIKQNTVDFIGLNYYSRTLVKPYTGGE
EEEHHHCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHCCCEEEEECHHHHHEECCCCCCC
TQLQFNHSGKKGESKVLIKNWFEQVKDPANETTEWDTEIYPKGLQDGLIEAYERYQLPLY
EEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEE
VTENGIGVREDVSVAKVDDAYRIAFMNDHINAIFNAIDAGCDVRGYYAWSPFDLYSWKNG
EECCCCCCCCCCCEEEECCCEEEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCC
VEKRYGLVAVDFENNQIRKPKASYYWFKEMIESQGKLIKRREF
HHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]