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The map label for this gene is ldh

Identifier: 226222839

GI number: 226222839

Start: 211076

End: 212017

Strand: Reverse

Name: ldh

Synonym: Lm4b_00208

Alternate gene names: 226222839

Gene position: 212017-211076 (Counterclockwise)

Preceding gene: 226222905

Following gene: 226222837

Centisome position: 7.28

GC content: 39.7

Gene sequence:

>942_bases
ATGAAAGATCATCAAAAAATTATTTTAGTTGGCGACGGAGCAGTTGGTTCTAGTTACGCATTTGCTTGTGTAAACTTAAG
CATTGGACAAGAATTTGGTATCATTGACATAGATAAAGACAGAACTATTGGGGATGCAATGGATTTAAGTCATGCCGTTC
CATTTTCTACACCGAAGAAAATCTACTCAGCAAATTATAGCGACTGCCACGATGCGGACTTAGTTGTTGTAACTGCCGGG
ACTGCACAAAAACCTGGTGAAACTCGTTTAGATTTAGTAAATCGTAATATTAAAATCATGAAAGGCATCGTGGATGAAGT
AATGGCTAGCGGATTTGACGGTATCTTTTTAATCGCTTCTAACCCAGTAGATATCTTAACTTACGCTACATGGAAATTCT
CCGGTCTTCCAAAAGAACGTGTTATCGGTTCTGGAACAAGCCTTGATACAGCACGTTTCCGTATGTCAATTGCCGACTAT
CTAAAAGTAGATGCTCGTAACGTCCATGGTTACATCCTTGGCGAACACGGCGATACAGAATTCCCAGCATGGAGCCACAC
AACTGTCGGCGGTCTTCCAATCACTGAATGGATTAGCGAAGATGAACAAGGTGCAATGGATACTATTTTCGTAAGTGTTC
GTGATGCAGCTTATGAAATTATTAATAAAAAAGGCGCTACATTCTACGGTGTTGCTGCAGCTCTAGCTCGTATTACAAAG
GCAATTCTAAATAACGAAAATGCGATCTTACCACTTTCTGTTTATCTAGATGGTCATTATGGTATGAACGACATTTATAT
CGGCGCTCCCGCAGTCGTTAACCGTCAAGGCGTTCGCCATATTGTTGAAATGAATTTAAACGACAAAGAAAAAGAACAAA
TGAAAAACTCTGCAGATACACTTAAAAAAGTTCTAGACGACGCAATGAAACAAATCGACTAA

Upstream 100 bases:

>100_bases
TATAACAACTTTTTTAAATTCAGGACGTTCAACGCTTCACAAATGAAAAATTCATGTTATACTGTACTAGTAAATAGATT
TTTTATAGGAGGAATTCGAA

Downstream 100 bases:

>100_bases
TATTCTCAAATCCTAAAAGCATGCGCCTTCGCATGCTTTTTTTGTTATACTTAATTTATTCGAGAGGAGTGACGCGTACG
ATTACTTTTGACCAACTTAA

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 1 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKDHQKIILVGDGAVGSSYAFACVNLSIGQEFGIIDIDKDRTIGDAMDLSHAVPFSTPKKIYSANYSDCHDADLVVVTAG
TAQKPGETRLDLVNRNIKIMKGIVDEVMASGFDGIFLIASNPVDILTYATWKFSGLPKERVIGSGTSLDTARFRMSIADY
LKVDARNVHGYILGEHGDTEFPAWSHTTVGGLPITEWISEDEQGAMDTIFVSVRDAAYEIINKKGATFYGVAAALARITK
AILNNENAILPLSVYLDGHYGMNDIYIGAPAVVNRQGVRHIVEMNLNDKEKEQMKNSADTLKKVLDDAMKQID

Sequences:

>Translated_313_residues
MKDHQKIILVGDGAVGSSYAFACVNLSIGQEFGIIDIDKDRTIGDAMDLSHAVPFSTPKKIYSANYSDCHDADLVVVTAG
TAQKPGETRLDLVNRNIKIMKGIVDEVMASGFDGIFLIASNPVDILTYATWKFSGLPKERVIGSGTSLDTARFRMSIADY
LKVDARNVHGYILGEHGDTEFPAWSHTTVGGLPITEWISEDEQGAMDTIFVSVRDAAYEIINKKGATFYGVAAALARITK
AILNNENAILPLSVYLDGHYGMNDIYIGAPAVVNRQGVRHIVEMNLNDKEKEQMKNSADTLKKVLDDAMKQID
>Mature_313_residues
MKDHQKIILVGDGAVGSSYAFACVNLSIGQEFGIIDIDKDRTIGDAMDLSHAVPFSTPKKIYSANYSDCHDADLVVVTAG
TAQKPGETRLDLVNRNIKIMKGIVDEVMASGFDGIFLIASNPVDILTYATWKFSGLPKERVIGSGTSLDTARFRMSIADY
LKVDARNVHGYILGEHGDTEFPAWSHTTVGGLPITEWISEDEQGAMDTIFVSVRDAAYEIINKKGATFYGVAAALARITK
AILNNENAILPLSVYLDGHYGMNDIYIGAPAVVNRQGVRHIVEMNLNDKEKEQMKNSADTLKKVLDDAMKQID

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI291575128, Length=310, Percent_Identity=38.7096774193548, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI4557032, Length=310, Percent_Identity=38.7096774193548, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=36.1563517915309, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI47059044, Length=307, Percent_Identity=37.4592833876222, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI221136809, Length=307, Percent_Identity=37.4592833876222, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI9257228, Length=308, Percent_Identity=39.2857142857143, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI4504973, Length=308, Percent_Identity=39.2857142857143, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI5031857, Length=304, Percent_Identity=36.8421052631579, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI260099723, Length=304, Percent_Identity=36.8421052631579, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI260099727, Length=192, Percent_Identity=43.75, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI260099725, Length=192, Percent_Identity=43.75, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI207028494, Length=205, Percent_Identity=35.609756097561, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI103472011, Length=302, Percent_Identity=26.158940397351, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI103472015, Length=184, Percent_Identity=29.8913043478261, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1789632, Length=311, Percent_Identity=26.3665594855305, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535107, Length=292, Percent_Identity=38.6986301369863, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17554310, Length=291, Percent_Identity=27.1477663230241, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17136226, Length=307, Percent_Identity=37.785016286645, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45550422, Length=304, Percent_Identity=29.2763157894737, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24647881, Length=277, Percent_Identity=28.5198555956679, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34192; Mature: 34192

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDHQKIILVGDGAVGSSYAFACVNLSIGQEFGIIDIDKDRTIGDAMDLSHAVPFSTPKK
CCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCEEECCCCCCCCHHHHHCCCCCCCCCHH
IYSANYSDCHDADLVVVTAGTAQKPGETRLDLVNRNIKIMKGIVDEVMASGFDGIFLIAS
HHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEC
NPVDILTYATWKFSGLPKERVIGSGTSLDTARFRMSIADYLKVDARNVHGYILGEHGDTE
CCCEEEEEEEEEECCCCHHHEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
FPAWSHTTVGGLPITEWISEDEQGAMDTIFVSVRDAAYEIINKKGATFYGVAAALARITK
CCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AILNNENAILPLSVYLDGHYGMNDIYIGAPAVVNRQGVRHIVEMNLNDKEKEQMKNSADT
HHHCCCCCEEEEEEEEECCCCCCEEEECCCHHHCHHHHHHHEECCCCCHHHHHHHHHHHH
LKKVLDDAMKQID
HHHHHHHHHHHCC
>Mature Secondary Structure
MKDHQKIILVGDGAVGSSYAFACVNLSIGQEFGIIDIDKDRTIGDAMDLSHAVPFSTPKK
CCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCEEECCCCCCCCHHHHHCCCCCCCCCHH
IYSANYSDCHDADLVVVTAGTAQKPGETRLDLVNRNIKIMKGIVDEVMASGFDGIFLIAS
HHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEC
NPVDILTYATWKFSGLPKERVIGSGTSLDTARFRMSIADYLKVDARNVHGYILGEHGDTE
CCCEEEEEEEEEECCCCHHHEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
FPAWSHTTVGGLPITEWISEDEQGAMDTIFVSVRDAAYEIINKKGATFYGVAAALARITK
CCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AILNNENAILPLSVYLDGHYGMNDIYIGAPAVVNRQGVRHIVEMNLNDKEKEQMKNSADT
HHHCCCCCEEEEEEEEECCCCCCEEEECCCHHHCHHHHHHHEECCCCCHHHHHHHHHHHH
LKKVLDDAMKQID
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 11683362 [H]