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The map label for this gene is metS

Identifier: 226222806

GI number: 226222806

Start: 172466

End: 174460

Strand: Direct

Name: metS

Synonym: Lm4b_00174

Alternate gene names: 226222806

Gene position: 172466-174460 (Clockwise)

Preceding gene: 226222805

Following gene: 226222807

Centisome position: 5.92

GC content: 38.6

Gene sequence:

>1995_bases
TTGCCTGAAGAGAAAAATACGTTTTATATTACCACACCAATCTATTATCCAAGCGGAAAAGCGCATATCGGCCATGCTTA
TACGACTGTTGCGGGGGACGCGATGGCTCGTTATAAACGCTTAAAAGGATATGATGTGTTTTACTTAACTGGAACAGATG
AGCACGGTCAAAAAATCCAAGCAAAAGCGAAAGAACGCGGAATTTCTGAACAAGAATACGTGGATGAAATCGCAGAAGGT
TTCCAAGAACTTTGGAAAAAACTAGAAATTTCTAATACGGATTTTATTCGTACAACACAAGACCGTCATAAAACATCGGT
TGAAAAAATCTTTGAACAACTTTTAGAACAAGGCGACATTTACTTAGGTGAATACGAAGGTTGGTACTCTGTTTCTGATG
AAGAATACTTTACAGAAACACAGTTAGAAGAAGTATATAAAGATGAAAATGGTAAAGTAATCGGCGGAAAAGCCCCAAGT
GGCAATGAAGTCGAACTAGTGAAAGAAGAATCCTACTTCTTCCGTATGAGCAAATATGCGGACCGTTTAGTAGAATATTA
TAATTCCCATCCAGAATTTATCCTTCCTGAATCTAGAAAAAATGAAATGATTAATAATTTCATCAAACCTGGTTTGGAAG
ATTTAGCTGTATCTAGAACAACTTTTGATTGGGGTATTAAAGTTCCTGGCAATCCAAAACACGTTGTTTATGTATGGATT
GACGCGCTTTCTAACTATATTACTGCGCTTGGGTATAATACGGACAATGACACGAAATTCCAAAAATACTGGCCTGCTGA
TGTACAAATCGTTGGGAAAGAAATCGTTCGTTTCCACACGATTTATTGGCCAATTATGCTGATGGCACTAGACTTACCAC
TTCCAAAAATGGTGTTCGGCCACGGCTGGATTCTAATGAAAGATGGTAAAATGTCTAAATCCAAAGGGAATGTAGTAGAT
CCTTATATGCTGATTGACCGCTACGGCCTAGATGCGCTTCGTTATTACTTATTACGCGAAGTTCCATTTGGTTCAGATGG
TTTATTTACACCGGAAGACTTTGTTGATCGTGTGAACTTTGATCTTGCCAACGATTTAGGAAACTTACTTAATCGTACAG
TAGCAATGATTAATAAGTACTTTGATGGCGAAATTCCGGCTTATCAAGGCAATGTAACAGAATTCGATCAAACCTTAGTG
GATTTCAAAAACAATGTGGTGAAGGAATATGAAGGTAGCATGGATCACATGCAATTCTCTGTAGCTTTAAACCAACTGTG
GTCACTAATTTCTCGTACAAATAAATATATCGATGAAACCGCTCCATGGACACTTGCCAAAGACGAAGATAAACGCACAG
AACTTGCGAGCGTAATGACTCATTTAGCAGAAAACTTACGTATTATCGCAGTACTTTTGCAACCATTCTTAACAAGAACT
CCAGGCGAAATCTTCTTACAACTAGGTTTACAAGAAGAAAACTTGAAAAAATGGGATAGCATCTATGGATACGGTGAAAT
TCCAGCTGGCACAACAGTGGTGAAAAAAGGTACGCCAATTTTCCCAAGACTTGAGGCAGAAGTAGAAGTTACTTATATCC
AAGATGAAATGAAAGGCTCTGCGCCAGCTCCTGCCAAAGAAGCGGCGGAAGTTGAAGCGCTTGAAACACCGCAAATCGGC
ATTGAAGATTTCGATAAAATCGATCTTCGTGTTGCAGAAGTAAAACAAGTAGATAAAGTGAAAAAAGCAGATAAACTTCT
TTGTTTCCAATTAGACTTAGGGGAAGGAAAACTGCGCCAAGTGCTTTCAGGCATTGCAGAATTCTATCAACCAGAAGAAC
TAATCGGCAAAAAAGTTATCGTCGTTTCTAACTTAAAACCTGTAAAACTTCGCGGACTAATGAGTGAAGGAATGATACTC
TCAGGCGAAAAAGATGGTAAGCTAAGTGTAATCGAAGCAAGTAGCGCACTTCCAAATGGTGCAAAAGTAAAATAG

Upstream 100 bases:

>100_bases
TTTTAATCGGCGTTGCAAAAGGTGCTTAATTAATTATTTCGTTTAGCGCGAAAAATGATATAATGATACTAATTGTTTTA
TTTTTAAGGAGGGAATTGTG

Downstream 100 bases:

>100_bases
TAAAAACGAGTCCTAATTCATTTGAGTTAGGGCTCCTTTTTTTCGCCTAAAAAAACTTTAAAAAAAAGATTGCATATTGC
AAACGATTACAATATAATGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MPEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKIQAKAKERGISEQEYVDEIAEG
FQELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLEQGDIYLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAPS
GNEVELVKEESYFFRMSKYADRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVWI
DALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVFGHGWILMKDGKMSKSKGNVVD
PYMLIDRYGLDALRYYLLREVPFGSDGLFTPEDFVDRVNFDLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTEFDQTLV
DFKNNVVKEYEGSMDHMQFSVALNQLWSLISRTNKYIDETAPWTLAKDEDKRTELASVMTHLAENLRIIAVLLQPFLTRT
PGEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLEAEVEVTYIQDEMKGSAPAPAKEAAEVEALETPQIG
IEDFDKIDLRVAEVKQVDKVKKADKLLCFQLDLGEGKLRQVLSGIAEFYQPEELIGKKVIVVSNLKPVKLRGLMSEGMIL
SGEKDGKLSVIEASSALPNGAKVK

Sequences:

>Translated_664_residues
MPEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKIQAKAKERGISEQEYVDEIAEG
FQELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLEQGDIYLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAPS
GNEVELVKEESYFFRMSKYADRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVWI
DALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVFGHGWILMKDGKMSKSKGNVVD
PYMLIDRYGLDALRYYLLREVPFGSDGLFTPEDFVDRVNFDLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTEFDQTLV
DFKNNVVKEYEGSMDHMQFSVALNQLWSLISRTNKYIDETAPWTLAKDEDKRTELASVMTHLAENLRIIAVLLQPFLTRT
PGEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLEAEVEVTYIQDEMKGSAPAPAKEAAEVEALETPQIG
IEDFDKIDLRVAEVKQVDKVKKADKLLCFQLDLGEGKLRQVLSGIAEFYQPEELIGKKVIVVSNLKPVKLRGLMSEGMIL
SGEKDGKLSVIEASSALPNGAKVK
>Mature_663_residues
PEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKIQAKAKERGISEQEYVDEIAEGF
QELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLEQGDIYLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAPSG
NEVELVKEESYFFRMSKYADRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVWID
ALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVFGHGWILMKDGKMSKSKGNVVDP
YMLIDRYGLDALRYYLLREVPFGSDGLFTPEDFVDRVNFDLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTEFDQTLVD
FKNNVVKEYEGSMDHMQFSVALNQLWSLISRTNKYIDETAPWTLAKDEDKRTELASVMTHLAENLRIIAVLLQPFLTRTP
GEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLEAEVEVTYIQDEMKGSAPAPAKEAAEVEALETPQIGI
EDFDKIDLRVAEVKQVDKVKKADKLLCFQLDLGEGKLRQVLSGIAEFYQPEELIGKKVIVVSNLKPVKLRGLMSEGMILS
GEKDGKLSVIEASSALPNGAKVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=541, Percent_Identity=34.5656192236599, Blast_Score=313, Evalue=4e-85,
Organism=Homo sapiens, GI14043022, Length=619, Percent_Identity=26.4943457189015, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI4507947, Length=109, Percent_Identity=35.7798165137615, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788432, Length=682, Percent_Identity=28.2991202346041, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1789455, Length=107, Percent_Identity=36.4485981308411, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI212645096, Length=510, Percent_Identity=33.921568627451, Blast_Score=278, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI71992781, Length=400, Percent_Identity=32.75, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17540794, Length=526, Percent_Identity=26.0456273764259, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71997510, Length=188, Percent_Identity=31.3829787234043, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71997517, Length=188, Percent_Identity=31.3829787234043, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321610, Length=516, Percent_Identity=33.5271317829457, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6321704, Length=482, Percent_Identity=25.3112033195021, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI21358483, Length=497, Percent_Identity=33.1991951710262, Blast_Score=287, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24655311, Length=588, Percent_Identity=26.7006802721088, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21357223, Length=76, Percent_Identity=42.1052631578947, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24651924, Length=97, Percent_Identity=36.0824742268041, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75655; Mature: 75524

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKIQ
CCCCCCEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
AKAKERGISEQEYVDEIAEGFQELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLEQGDI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCE
YLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAPSGNEVELVKEESYFFRMSKYA
EEECCCCEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCHHHHHHHHHH
DRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVWI
HHHHHHHCCCCCEECCCCHHHHHHHHHHCCCHHHHHEECEEEECEEECCCCCCEEEEEEH
DALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVFG
HHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHC
HGWILMKDGKMSKSKGNVVDPYMLIDRYGLDALRYYLLREVPFGSDGLFTPEDFVDRVNF
CCEEEEECCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCC
DLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTEFDQTLVDFKNNVVKEYEGSMDHMQFS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VALNQLWSLISRTNKYIDETAPWTLAKDEDKRTELASVMTHLAENLRIIAVLLQPFLTRT
HHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLEAEVEVTYIQDEMKGS
CCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEEHHHCCC
APAPAKEAAEVEALETPQIGIEDFDKIDLRVAEVKQVDKVKKADKLLCFQLDLGEGKLRQ
CCCCHHHHHHHHHCCCCCCCCCCCCHHHEEHHHHHHHHHHHHHCCEEEEEEECCCHHHHH
VLSGIAEFYQPEELIGKKVIVVSNLKPVKLRGLMSEGMILSGEKDGKLSVIEASSALPNG
HHHHHHHHCCCHHHHCCEEEEEECCCCCHHHHHHHCCEEEECCCCCCEEEEECCCCCCCC
AKVK
CCCC
>Mature Secondary Structure 
PEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKIQ
CCCCCEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
AKAKERGISEQEYVDEIAEGFQELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLEQGDI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCE
YLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAPSGNEVELVKEESYFFRMSKYA
EEECCCCEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCHHHHHHHHHH
DRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVWI
HHHHHHHCCCCCEECCCCHHHHHHHHHHCCCHHHHHEECEEEECEEECCCCCCEEEEEEH
DALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVFG
HHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHC
HGWILMKDGKMSKSKGNVVDPYMLIDRYGLDALRYYLLREVPFGSDGLFTPEDFVDRVNF
CCEEEEECCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCC
DLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTEFDQTLVDFKNNVVKEYEGSMDHMQFS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VALNQLWSLISRTNKYIDETAPWTLAKDEDKRTELASVMTHLAENLRIIAVLLQPFLTRT
HHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLEAEVEVTYIQDEMKGS
CCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEEHHHCCC
APAPAKEAAEVEALETPQIGIEDFDKIDLRVAEVKQVDKVKKADKLLCFQLDLGEGKLRQ
CCCCHHHHHHHHHCCCCCCCCCCCCHHHEEHHHHHHHHHHHHHCCEEEEEEECCCHHHHH
VLSGIAEFYQPEELIGKKVIVVSNLKPVKLRGLMSEGMILSGEKDGKLSVIEASSALPNG
HHHHHHHHCCCHHHHCCEEEEEECCCCCHHHHHHHCCEEEECCCCCCEEEEECCCCCCCC
AKVK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA