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The map label for this gene is endA [H]

Identifier: 225861781

GI number: 225861781

Start: 1823146

End: 1823970

Strand: Reverse

Name: endA [H]

Synonym: SPT_1943

Alternate gene names: 225861781

Gene position: 1823970-1823146 (Counterclockwise)

Preceding gene: 225861782

Following gene: 225861780

Centisome position: 86.36

GC content: 43.52

Gene sequence:

>825_bases
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGCCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAATGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Upstream 100 bases:

>100_bases
CATGGGCTATCCTGTCTCCAGCAAAATGGCAGGAATTGATTCATAAATTTACAGGAAATTAGGCTGGAGAACCAGCCTTT
TTCTAAAGATAAGGAGAAAT

Downstream 100 bases:

>100_bases
AAGATAAGCCTAAACTCCTATGTCACTTATGGATGTAGGAGTTCTTTTTACTAGTTTAAGCAGGGCTAGAATAGGTACTA
AGAAAAAATAGTAACTTCTA

Product: DNA-entry nuclease (Competence-specific nuclease)

Products: NA

Alternate protein names: Competence-specific nuclease [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Sequences:

>Translated_274_residues
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ
>Mature_274_residues
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Specific function: By degrading DNA that enters the cell, plays a role in the competence of cells to be transformed [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: 3.1.30.-

Molecular weight: Translated: 29918; Mature: 29918

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQAPEAPSQALAESVLTD
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
AVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSNA
HHHHHHCCCEEECCCEEEEECCCCCCCCEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHH
TRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNP
HHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
KNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
CEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHC
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ
CCCCCCCEEEEEECCCCCCCCEEEEECCCEEECC
>Mature Secondary Structure
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQAPEAPSQALAESVLTD
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
AVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSNA
HHHHHHCCCEEECCCEEEEECCCCCCCCEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHH
TRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNP
HHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
KNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
CEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHC
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ
CCCCCCCEEEEEECCCCCCCCEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2359120; 11463916 [H]