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The map label for this gene is ply [H]

Identifier: 225861716

GI number: 225861716

Start: 1758139

End: 1759554

Strand: Reverse

Name: ply [H]

Synonym: SPT_1872

Alternate gene names: 225861716

Gene position: 1759554-1758139 (Counterclockwise)

Preceding gene: 225861717

Following gene: 225861715

Centisome position: 83.31

GC content: 41.95

Gene sequence:

>1416_bases
ATGGCAAATAAAGCAGTAAATGACTTTATACTAGCTATGAATTACGATAAAAAGAAACTCTTGACCCATCAGGGAGAAAG
TATTGAAAATCGTTTCATCAAAGAGGGTAATCAGCTACCCGATGAGTTTGTTGTTATCGAAAGAAAGAAGCGGAGCTTGT
CGACAAATACAAGTGATATTTCTGTAACAGCTACCAACGACAGTCGCCTCTATCCTGGAGCACTTCTCGTAGTGGATGAG
ACCTTGTTAGAGAATAATCCCACTCTTCTTGCGGTCGATCGTGCTCCGATGACTTATAGTATTGATTTGCCTGGTTTGGC
AAGTAGCGATAGCTTTCTCCAAGTGGAAGACCCCAGCAATTCAAGTGTTCGCGGAGCGGTAAACGATTTGTTGGCTAAGT
GGCATCAAGATTATGGTCAGGTCAATAATGTCCCAGCTAGAATGCAGTATGAAAAAATCACGGCTCACAGCATGGAACAA
CTCAAGGTCAAGTTTGGTTCTGACTTTGAAAAGACAGGGAATTCTCTTGATATTGATTTTAACTCTGTCCATTCAGGCGA
AAAGCAGATTCAGATTGTTAATTTTAAGCAGATTTATTATACAGTCAGCGTAGACGCTGTTAAAAATCCAGGAGATGTGT
TTCAAGATACTGTAACGGTAGAGGATTTAAAACAGAGAGGAATTTCTGCAGAGCGTCCTTTGGTCTATATTTCGAGTGTT
GCTTATGGGCGCCAAGTCTATCTCAAGTTGGAAACCACGAGTAAGAGTGATGAAGTAGAGGCTGCTTTTGAAGCTTTGAT
AAAAGGAGTCAAGGTAGCTCCTCAGACAGAGTGGAAGCAGATTTTGGACAATACAGAAGTGAAGGCGGTTATTTTAGGGG
GCGACCCAAGTTCGGGTGCCCGAGTTGTAACAGGCAAGGTGGATATGGTAGAGGACTTGATTCAAGAAGGCAGTCGCTTT
ACAGCAGATCATCCAGGCTTGCCGATTTCCTATACAACTTCTTTTTTACGTGACAATGTAGTTGCGACCTTTCAAAATAG
TACAGACTATGTTGAGACTAAGGTTACAGCTTACAGAAACGGAGATTTACTGCTGGATCATAGTGGTGCCTATGTTGCCC
AATATTATATTACTTGGAATGAATTATCCTATGATCATCAAGGTAAGGAAGTCTTGACTCCTAAGGCTTGGGACAGAAAT
GGGCAGGATTTAACGGCTCACTTTACCACTAGTATTCCTTTAAAAGGGAATGTTCGTAATCTCTCTGTCAAAATTAGAGA
GTGTACCGGGCTTGCCTGGGAATGGTGGCGTACGGTTTATGAAAAAACCGATTTGCCACTAGTGCGTAAGCGGACGATTT
CTATTTGGGGAACAACTCTCTATCCTCAGGTAGAAGATAAGGTAGAAAATGACTAG

Upstream 100 bases:

>100_bases
TACGAGGAAATTTTGGATCACTTAGTCCAATCACGGCTGATTTCGCTGAACAAGTCTGAGTTTATGCAACTCAATCCAGG
AACTTATTAGGAGGTAGAAG

Downstream 100 bases:

>100_bases
GAGAGGAGAATGCTTGCGACAAAAAGAGGCGATGATCTCTCTGCAGATATCTGAAAGTTTATCTCTTGCCTAGCGATTTC
CATTGGAGTATGCTCGTGAT

Product: pneumolysin (Thiol-activated cytolysin)

Products: NA

Alternate protein names: Thiol-activated cytolysin [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDE
TLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSV
AYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRF
TADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRN
GQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

Sequences:

>Translated_471_residues
MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDE
TLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSV
AYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRF
TADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRN
GQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
>Mature_470_residues
ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDET
LLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQL
KVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVA
YGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFT
ADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNG
QDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

Specific function: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation o

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein (By similarity). Note=Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiol-activated cytolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001869 [H]

Pfam domain/function: PF01289 Thiol_cytolysin [H]

EC number: NA

Molecular weight: Translated: 52899; Mature: 52768

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00481 THIOL_CYTOLYSINS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDI
CCCCCCCCEEEEECCCHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCCEE
SVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSN
EEEECCCCEECCCEEEEEEHHHHCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEECCCC
SSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDF
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHCCCEEEEEE
NSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSV
CCCCCCCCEEEEEEEEEEEEEEEEECCCCCCHHHHCCEEHHHHHHCCCCCCCCEEEEEEE
AYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGA
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCC
RVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRN
EEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCEEEEEEEEEEC
GDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRN
CCEEEECCCCEEEEEEEEEEECCCCCCCCEECCCCCCCCCCCEEEEEEEEECCCCCCCEE
LSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
EEEEEEECCCCHHHHHHHHHHCCCCCEEHEEEEEEEEEEECCCHHHCCCCC
>Mature Secondary Structure 
ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDI
CCCCCCCEEEEECCCHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCCEE
SVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSN
EEEECCCCEECCCEEEEEEHHHHCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEECCCC
SSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDF
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHCCCEEEEEE
NSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSV
CCCCCCCCEEEEEEEEEEEEEEEEECCCCCCHHHHCCEEHHHHHHCCCCCCCCEEEEEEE
AYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGA
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCC
RVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRN
EEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCEEEEEEEEEEC
GDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRN
CCEEEECCCCEEEEEEEEEEECCCCCCCCEECCCCCCCCCCCEEEEEEEEECCCCCCCEE
LSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
EEEEEEECCCCHHHHHHHHHHCCCCCEEHEEEEEEEEEEECCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3552992 [H]