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The map label for this gene is rumA2 [C]

Identifier: 225861693

GI number: 225861693

Start: 1740485

End: 1741840

Strand: Reverse

Name: rumA2 [C]

Synonym: SPT_1846

Alternate gene names: 225861693

Gene position: 1741840-1740485 (Counterclockwise)

Preceding gene: 225861696

Following gene: 225861690

Centisome position: 82.47

GC content: 37.76

Gene sequence:

>1356_bases
ATGAATCTGAAAGTGAAACAAAAAATACCATTAAAAATCAAGCGCATGGGAATTAACGGTGAGGGAATCGGCTTTTACCA
AAAAACATTAGTCTTTGTACCAGGAGCTCTCAAAGGCGAAGATATCTATTGTCAGATTACTTCTATTAGACGCAACTTTG
TTGAAGCAAAATTACTGAAGGTCAACAAGAAGTCTAAATTTCGAATTGTGCCATCTTGTACTATTTATAATGAATGCGGA
GGCTGCCAAATCATGCACCTGCATTATGATAAGCAGCTGGAGTTCAAGACGGACTTACTTCATCAAGCGCTGAAAAAATT
TGCTCCTGCAGGATATGAAAATTATGAAATTCGTCCAACTATTGGAATGCAGGAACCAAAATATTACAGAGCTAAGTTAC
AATTTCAGACTCGAAAATTTAAAAATCAGGTCAAGGCGGGCTTATATGCACAAAACTCTCACTATTTAGTAGAGTTGAAA
GACTGCCTGGTACAAGATAAGGAAACCCAAGTGATTGCTAATCGCTTAGCAGAATTACTTACTTATCACCAGATTCCAAT
CACGGATGAGAGAAAAGTTCTAGGTGTCCGTACTATTATGGTCCGACGCGCGAGAAAGACCGGACAGGTTCAGATTATTA
TTGTTACAAACCGCCAGCTTAATTTAACTCAATTGGTAAAAGAGTTGGTTAAAGATTTCCCAGAAGTTGTGACAGTAGCT
GTTAATACAAATACAGCTAAAACCAGTGAGATATATGGTGAAAAGACAGAGATTATCTGGGGGCAAGAGAGTATTCAAGA
AGGTGTACTCAATTATGAATTTTCACTATCCCCTCGAGCTTTTTATCAACTAAATCCTGAGCAAACAGAAGTCCTCTATA
GCGAAGCAGTAAAAGCGCTGGATGTTGATAAAGAAGACCATTTGATTGACGCTTATTGTGGAGTTGGAACGATTGGATTT
GCCTTTGCAAAGAAAGTAAAAACACTCAGAGGTATGGATATTATTCCAGAAGCTATTGAAGATGCCAAGCGAAATGCTAA
AAGAATGGGATTTGACAATACTCATTATGAAGCTGGAACGGCAGAAGAGATTATTCCTCGTTGGTACAAGGAAGGCTACC
GAGCAGATGCTTTGATTATTGACCCACCACGTACAGGTCTGGATGATAAGTTATTAGATACTATTCTTACTTATGTACCA
GAAAAAATGGTTTATATTTCTTGTAATGTTTCGACCTTGGCTCGTGATTTGGTACGCTTAGTAGAAGTCTATGATCTTCA
TTATATCCAGTCGGTCGATATGTTCCCACATACAGCTCGAACTGAAGCTGTTGTAAAATTAATAAAAAAAGTTTAA

Upstream 100 bases:

>100_bases
GTCGCCATTATCAAGCTCCATCAGATAGAGTCTTTTTTTCTTTTCAAGTTTTGTGATTTTCATAGCTCTATTATAACTCA
AAATGTGATAAGATAGGGGT

Downstream 100 bases:

>100_bases
AAAAGGTGTTGACAAAGTTTGAAAAGACTGTATAATAGTAAGAGTTGAAAATAACAACTCAGGTCCGTTGGTCAAGGGGT
TAAGACACCGCCTTTTCACG

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECG
GCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVA
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGF
AFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIIDPPRTGLDDKLLDTILTYVP
EKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV

Sequences:

>Translated_451_residues
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECG
GCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVA
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGF
AFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIIDPPRTGLDDKLLDTILTYVP
EKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV
>Mature_451_residues
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECG
GCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVA
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGF
AFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIIDPPRTGLDDKLLDTILTYVP
EKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=210, Percent_Identity=32.3809523809524, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI51173878, Length=210, Percent_Identity=32.3809523809524, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI269784680, Length=277, Percent_Identity=26.7148014440433, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI269784676, Length=277, Percent_Identity=26.7148014440433, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI20149688, Length=277, Percent_Identity=26.7148014440433, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI269784678, Length=277, Percent_Identity=26.7148014440433, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI1789148, Length=453, Percent_Identity=26.0485651214128, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787083, Length=384, Percent_Identity=24.7395833333333, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1790403, Length=166, Percent_Identity=28.3132530120482, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71997438, Length=193, Percent_Identity=26.9430051813472, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322909, Length=432, Percent_Identity=23.1481481481481, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24666579, Length=422, Percent_Identity=26.5402843601896, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51757; Mature: 51757

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLK
CCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHE
VNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPT
ECCCCCEEEEECEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
IGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELKDCLVQDKETQVIANRLAELL
CCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEHHHHHCCCHHHHHHHHHHHHH
TYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVA
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHEEEE
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKAL
EECCCCCHHHHCCCCEEEEECHHHHHCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHH
DVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGT
CCCCCCCEEEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
AEEIIPRWYKEGYRADALIIDPPRTGLDDKLLDTILTYVPEKMVYISCNVSTLARDLVRL
HHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHH
VEVYDLHYIQSVDMFPHTARTEAVVKLIKKV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLK
CCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHE
VNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPT
ECCCCCEEEEECEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
IGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELKDCLVQDKETQVIANRLAELL
CCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEHHHHHCCCHHHHHHHHHHHHH
TYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVA
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHEEEE
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKAL
EECCCCCHHHHCCCCEEEEECHHHHHCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHH
DVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGT
CCCCCCCEEEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
AEEIIPRWYKEGYRADALIIDPPRTGLDDKLLDTILTYVPEKMVYISCNVSTLARDLVRL
HHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHH
VEVYDLHYIQSVDMFPHTARTEAVVKLIKKV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11544234 [H]