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The map label for this gene is gtfA [H]

Identifier: 225861685

GI number: 225861685

Start: 1724642

End: 1726084

Strand: Reverse

Name: gtfA [H]

Synonym: SPT_1815

Alternate gene names: 225861685

Gene position: 1726084-1724642 (Counterclockwise)

Preceding gene: 225861686

Following gene: 225861684

Centisome position: 81.72

GC content: 37.98

Gene sequence:

>1443_bases
ATGCCAATTCAAAATAAAACCATGTTGATTACCTATTCTGATAGCCTTGGAAATAATCTTAAAGACTTATATGATAATTT
GGAAGAGCATTTTGGAGATGCTATTGGAGGAGTTCACCTTTTACCATTTTTCCCATCAACAGGTGATCGTGGATTTGCGC
CAGTTGACTACGACGAAGTGGACTCAGCTTTTGGTGATTGGGAGGATGTTAAGCGTTTAGGTGAGAGATATTATCTTATG
TTTGATTTTATGATTAATCATATTTCTCGTCAATCCAAGTATTATAAGGACTATCAAGAAAAACATGAAGCCAGTGAATT
TAAAGATCTCTTTTTAAACTGGGATAAGTTTTGGCCAGAAAACCGTCCGACACAGTCTGATGTAGATTTAATTTACAAGC
GTAAGGATCGTGCACCAAAGCAAGAGATTGTGTTTGAAGATGGTTCAGTGGAACATTTGTGGAATACCTTTGGTGAGGAG
CAGATTGATCTTGATGTGACCAAAGAAGTAACTATGGAATTTATCCGTAAGACCATTCAGCACTTGGCAAGTAATGGGTG
TGATTTGATTCGTCTAGATGCCTTTGCTTATGCAGTGAAGAAATTGGATACTAATGATTTCTTTGTGGAACCAGATATTT
GGGATTTATTGGACAAAGTTCGAGATATCGCTGCTGAGTATGGGACAGAGCTTTTACCTGAGATTCATGAACACTATTCG
ATTCAGTTTAAAATAGCAGACCATGATTACTATGTTTATGATTTTGCTCTTCCAATGGTGACACTTTATACTCTTTACAG
TTCCAGAACAGAGCGTTTGGCTAAGTGGTTAAAGATGAGCCCGATGAAGCAATTTACGACGCTAGATACCCATGATGGGA
TTGGAGTAGTAGATGTCAAGGATATCCTGACCGATGAGGAGATTGACTATGCTTCAAATGAACTCTATAAGGTTGGAGCC
AATGTCAAACGTAAGTACTCTAGTGCCGAGTATAACAACTTAGATATCTACCAAATCAATTCAACCTACTATTCAGCGCT
TGGAGATGATGATGTCAAGTATTTTCTCGCTCGTCTAATTCAAGCTTTTGCCCCAGGTATTCCTCAGGTTTACTATGTGG
GTCTATTAGCAGGCAAGAATGACTTGAAATTATTAGAAGAAACTAAAGAAGGTCGAAATATTAATCGTCATTACTATAGC
AACGAGGAAATAGCAAAAGAAGTGCAACGATCTGTTGTGAAGGCCCTTCTCAATCTATTTTCTTTCCGTAACCGTTCAGA
AGCCTTTGATCTAGAAGGGACTACTGAGATAGAGACACCAACAGCTCACAGCATTGTAATCAAACGTCAAAATAAAGATA
AGTCCGTAACAGCAGTAGCAGAAATTGATTTGCAAAATCAGACTTATCGGGTAATTGAGAATGGAGTTGAAGTAACATTT
TGA

Upstream 100 bases:

>100_bases
ATTAGTACGAGTTTGATGATAAATAGAGATTTAAAATGAATTTTATTTATGAAGCCAGATCATATGAGACTTGGCTTCAA
TTAGAAAAAGGAGAGTAATG

Downstream 100 bases:

>100_bases
AGCCTTGATATGGTGGATAAAATAGGGTTTTTATTTTGGAAAACGTTTCCTTTGTTTTCAAATTGCTAAAAAAGTGGTAC
AATATAGGATAGCTTACTAT

Product: sucrose phosphorylase (Sucrose glucosyltransferase)(Glucosyltransferase-A) (GTF-A)

Products: NA

Alternate protein names: Glucosyltransferase-A; GTF-A; Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFFPSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLM
FDFMINHISRQSKYYKDYQEKHEASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLDKVRDIAAEYGTELLPEIHEHYS
IQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKWLKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGA
NVKRKYSSAEYNNLDIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGRNINRHYYS
NEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQNKDKSVTAVAEIDLQNQTYRVIENGVEVTF

Sequences:

>Translated_480_residues
MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFFPSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLM
FDFMINHISRQSKYYKDYQEKHEASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLDKVRDIAAEYGTELLPEIHEHYS
IQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKWLKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGA
NVKRKYSSAEYNNLDIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGRNINRHYYS
NEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQNKDKSVTAVAEIDLQNQTYRVIENGVEVTF
>Mature_479_residues
PIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFFPSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMF
DFMINHISRQSKYYKDYQEKHEASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEEQ
IDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLDKVRDIAAEYGTELLPEIHEHYSI
QFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKWLKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGAN
VKRKYSSAEYNNLDIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGRNINRHYYSN
EEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQNKDKSVTAVAEIDLQNQTYRVIENGVEVTF

Specific function: Intracellular catabolism of sucrose. Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI87081873, Length=486, Percent_Identity=30.4526748971193, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377
- InterPro:   IPR022527 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 55879; Mature: 55748

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFFPSTGDRGFAPVDYDEV
CCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHH
DSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKHEASEFKDLFLNWDKFWPE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCC
NRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEEQIDLDVTKEVTMEFIRKTIQ
CCCCCCHHHHHHHHHCCCCCHHEEECCCCHHHHHHHCCCCEECCHHHHHHHHHHHHHHHH
HLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLDKVRDIAAEYGTELLPEIHEHYS
HHHCCCCCEEEHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCEE
IQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKWLKMSPMKQFTTLDTHDGIGVVDVK
EEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEHH
DILTDEEIDYASNELYKVGANVKRKYSSAEYNNLDIYQINSTYYSALGDDDVKYFLARLI
HHHCCCCCCHHCCHHEECCCHHHHHHCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHH
QAFAPGIPQVYYVGLLAGKNDLKLLEETKEGRNINRHYYSNEEIAKEVQRSVVKALLNLF
HHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SFRNRSEAFDLEGTTEIETPTAHSIVIKRQNKDKSVTAVAEIDLQNQTYRVIENGVEVTF
HHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
PIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFFPSTGDRGFAPVDYDEV
CCCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHH
DSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKHEASEFKDLFLNWDKFWPE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCC
NRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEEQIDLDVTKEVTMEFIRKTIQ
CCCCCCHHHHHHHHHCCCCCHHEEECCCCHHHHHHHCCCCEECCHHHHHHHHHHHHHHHH
HLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLDKVRDIAAEYGTELLPEIHEHYS
HHHCCCCCEEEHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCEE
IQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKWLKMSPMKQFTTLDTHDGIGVVDVK
EEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEHH
DILTDEEIDYASNELYKVGANVKRKYSSAEYNNLDIYQINSTYYSALGDDDVKYFLARLI
HHHCCCCCCHHCCHHEECCCHHHHHHCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHH
QAFAPGIPQVYYVGLLAGKNDLKLLEETKEGRNINRHYYSNEEIAKEVQRSVVKALLNLF
HHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SFRNRSEAFDLEGTTEIETPTAHSIVIKRQNKDKSVTAVAEIDLQNQTYRVIENGVEVTF
HHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3194228; 2967248; 12397186; 2971020 [H]