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The map label for this gene is treA [H]

Identifier: 225861676

GI number: 225861676

Start: 1708213

End: 1709838

Strand: Reverse

Name: treA [H]

Synonym: SPT_1800

Alternate gene names: 225861676

Gene position: 1709838-1708213 (Counterclockwise)

Preceding gene: 225861677

Following gene: 225861675

Centisome position: 80.95

GC content: 41.64

Gene sequence:

>1626_bases
ATGACACTTGATAAAGGAAAAGTAGTCTATCAAATCTATCCAAAATCTTACAAAGACACCACTGAAAACGGTATTGGGGA
TTTCCGTGGGATTATCGAAAAAATTCCCTATTTAGCCAAACTAGGTGTGGATATGGTCTGGCTCAATCCATTCTATCCAA
GCCCTCAACGGGATAATGGTTACGATATTTCAGATTATATGGCAGTGGATCCTCTTTTTGGTGATATGGCTGATTTTGAG
GAAATGGTGTGTGTCGGTAAAGAGCACAAGATTGACTTTATGCTGGACATGGTACTTAATCATTGTTCGACAGAGCATGA
ATGGTTTCAGAAAGCCCTAGCTGGTGATAAGTATTATCAAGACTTTTTCTTCATCCAAGACCAACCAACAGATTGGCAGT
CTAAGTTTGGTGGTTCTGCATGGACGCCTTTCGGGGATACTGGGAAATACTATCTTCACCTATTTGATGAGACCCAGGCT
GACCTTAACTGGCGCAATTCCAATGTCCGTAAGGAGCTTTTCAAGGTTGTTAATTTCTGGCGCGACAAGGGTATCAAAGG
TTTCCGATTTGATGTGATCAATTTGATCGGTAAGGACGAGGTTTCAGTGGACTGTCCTGAAAATGAAGGGAAGCCAGCTT
ATACAGACAAACCCATCGTTCATAACTATTTGCGTATGATGAACCAAGCCACTTTTGGATCTGACGATAGCTTTATGACA
GTTGGGGAAATGTCTTCTACCACCATGGAAAACTGTGTCCTTTATTCGTCGCCTGATCGTCAGGAATTATCCATGACCTT
TAATTTTCATCACCTCAAGGTGGACTATAAGGATGGACAAAAGTGGACCTTGGCTCCCTTTGATTTTGAAGAGTTGAAGA
GTCTTTACCATAGTTGGGGTAAGGAAATGAGTGATAAAGACGGTTGGAGTGCCCTCTTTTGGAACAATCACGATCAACCA
CGTGCCTTGAACCGTTTTGTCGATATTCAAAACTTCCGCAAGGAAGGAGCCACCATGCTAGCGGCCAGCATTCACCTGTC
ACGTGGGACACCTTATATCTACATGGGTGAGGAAATCGGGATGATTGACCCAGACTATGATTCTATGGCTGATTATGTGG
ATGTCGAATCGCTTAATGCCTATCAGATGCTCTTAGAAGAAGGAAAGAGTCAGCAAGAAGCCTTCCAGATTATTCAAGCT
AAGTCGCGTGATAATTCACGCATTCCCATGCAGTGGGATGCTTCGGAAAATGCAGGATTTTCAACAGGCACTCCTTGGTT
GAAAGCTGGTAAATCCTATAAAGACATCAATGTAGAAAATGAAATCCAGGGTCCGATTTTTACCTTCTATCAAGACTTGA
TTCGACTGCGTAAGGAAATGCCAATCATTTCAGAAGGAAGTTACAAACCAGCCTTTGAAGATAGCAAACAAGTCTACGCT
TTTGAACGCCAGTTTGAGGATCAAAAGTTACTAGTGCTCAATAACTTTTATGCTAAAGAAGTGGAAATCGACTTGCCAGC
AGTCTACCAAAATGGACAGATTTTGATATCAAACTATGAAGATGCAGAAGTGTCTGAGAAAATTCTACTCAAACCCTATC
AAACACTGGCTATTTATGTAAATTAA

Upstream 100 bases:

>100_bases
GCTTCCTAGTTTGTTCTTTGATTTTCATTGAGTATAATTGAAATAGCACAAATAGGGACGGAAGACTGCTTCCTCCCTTT
TATCAGAAAAGGAGAAACAG

Downstream 100 bases:

>100_bases
TACTCTTCGCAAATCTCTTCAAACCGTGTCAGTTTTATCTGCAACCTCAAAGCGGTGCTTTGAGCAACCTGTGGCTAGCT
TCCTAGTTTGCTCTTTGATT

Product: trehalose-6-phosphate hydrolase

Products: NA

Alternate protein names: Alpha,alpha-phosphotrehalase [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVDMVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFE
EMVCVGKEHKIDFMLDMVLNHCSTEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
DLNWRNSNVRKELFKVVNFWRDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKPIVHNYLRMMNQATFGSDDSFMT
VGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDYKDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQP
RALNRFVDIQNFRKEGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQEAFQIIQA
KSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFYQDLIRLRKEMPIISEGSYKPAFEDSKQVYA
FERQFEDQKLLVLNNFYAKEVEIDLPAVYQNGQILISNYEDAEVSEKILLKPYQTLAIYVN

Sequences:

>Translated_541_residues
MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVDMVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFE
EMVCVGKEHKIDFMLDMVLNHCSTEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
DLNWRNSNVRKELFKVVNFWRDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKPIVHNYLRMMNQATFGSDDSFMT
VGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDYKDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQP
RALNRFVDIQNFRKEGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQEAFQIIQA
KSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFYQDLIRLRKEMPIISEGSYKPAFEDSKQVYA
FERQFEDQKLLVLNNFYAKEVEIDLPAVYQNGQILISNYEDAEVSEKILLKPYQTLAIYVN
>Mature_540_residues
TLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVDMVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEE
MVCVGKEHKIDFMLDMVLNHCSTEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQAD
LNWRNSNVRKELFKVVNFWRDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKPIVHNYLRMMNQATFGSDDSFMTV
GEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDYKDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPR
ALNRFVDIQNFRKEGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQEAFQIIQAK
SRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFYQDLIRLRKEMPIISEGSYKPAFEDSKQVYAF
ERQFEDQKLLVLNNFYAKEVEIDLPAVYQNGQILISNYEDAEVSEKILLKPYQTLAIYVN

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=524, Percent_Identity=27.6717557251908, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1790687, Length=537, Percent_Identity=46.7411545623836, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1786604, Length=179, Percent_Identity=27.9329608938547, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081873, Length=160, Percent_Identity=30, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI32565753, Length=196, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25147709, Length=197, Percent_Identity=28.4263959390863, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321726, Length=576, Percent_Identity=34.5486111111111, Blast_Score=311, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6322241, Length=576, Percent_Identity=34.5486111111111, Blast_Score=307, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6322021, Length=576, Percent_Identity=34.5486111111111, Blast_Score=307, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6324416, Length=576, Percent_Identity=34.5486111111111, Blast_Score=306, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6321731, Length=568, Percent_Identity=34.330985915493, Blast_Score=300, Evalue=3e-82,
Organism=Saccharomyces cerevisiae, GI6319776, Length=568, Percent_Identity=34.1549295774648, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6322245, Length=562, Percent_Identity=31.8505338078292, Blast_Score=281, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24586591, Length=567, Percent_Identity=32.6278659611993, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24586593, Length=530, Percent_Identity=31.6981132075472, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24583745, Length=579, Percent_Identity=30.2245250431779, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24583747, Length=537, Percent_Identity=32.7746741154562, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24583749, Length=537, Percent_Identity=32.7746741154562, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI221330053, Length=585, Percent_Identity=30.2564102564103, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45549022, Length=573, Percent_Identity=31.239092495637, Blast_Score=218, Evalue=7e-57,
Organism=Drosophila melanogaster, GI24586589, Length=541, Percent_Identity=31.4232902033272, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24586599, Length=537, Percent_Identity=30.5400372439479, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24586597, Length=532, Percent_Identity=30.0751879699248, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24586587, Length=577, Percent_Identity=25.6499133448873, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI281360393, Length=482, Percent_Identity=29.8755186721992, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR012769 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.93 [H]

Molecular weight: Translated: 62814; Mature: 62683

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVDMVWLNPFYPSPQRDNG
CCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCCEEEECCCCCCCCCCCC
YDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHCSTEHEWFQKALAGDKYYQ
CCHHHHEEECCHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
DFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQADLNWRNSNVRKELFKVVNFW
EEEEEECCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
RDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKPIVHNYLRMMNQATFGSDDSFMT
HHCCCCCEEEHHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEE
VGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDYKDGQKWTLAPFDFEELKSLYHSWG
ECCCCCCCHHCEEEECCCCCCEEEEEEEEEEEEEEECCCCEEEECCCCHHHHHHHHHHHC
KEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRKEGATMLAASIHLSRGTPYIYMGEEIG
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEECCCCC
MIDPDYDSMADYVDVESLNAYQMLLEEGKSQQEAFQIIQAKSRDNSRIPMQWDASENAGF
CCCCCHHHHHHHHCHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCC
STGTPWLKAGKSYKDINVENEIQGPIFTFYQDLIRLRKEMPIISEGSYKPAFEDSKQVYA
CCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEH
FERQFEDQKLLVLNNFYAKEVEIDLPAVYQNGQILISNYEDAEVSEKILLKPYQTLAIYV
HHHCCCCCEEEEEECCEEEEEEEECCEEECCCEEEEECCCCCHHHHHHHCCCCEEEEEEE
N
C
>Mature Secondary Structure 
TLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVDMVWLNPFYPSPQRDNG
CCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCCEEEECCCCCCCCCCCC
YDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHCSTEHEWFQKALAGDKYYQ
CCHHHHEEECCHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
DFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQADLNWRNSNVRKELFKVVNFW
EEEEEECCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
RDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKPIVHNYLRMMNQATFGSDDSFMT
HHCCCCCEEEHHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEE
VGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDYKDGQKWTLAPFDFEELKSLYHSWG
ECCCCCCCHHCEEEECCCCCCEEEEEEEEEEEEEEECCCCEEEECCCCHHHHHHHHHHHC
KEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRKEGATMLAASIHLSRGTPYIYMGEEIG
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEECCCCC
MIDPDYDSMADYVDVESLNAYQMLLEEGKSQQEAFQIIQAKSRDNSRIPMQWDASENAGF
CCCCCHHHHHHHHCHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCC
STGTPWLKAGKSYKDINVENEIQGPIFTFYQDLIRLRKEMPIISEGSYKPAFEDSKQVYA
CCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEH
FERQFEDQKLLVLNNFYAKEVEIDLPAVYQNGQILISNYEDAEVSEKILLKPYQTLAIYV
HHHCCCCCEEEEEECCEEEEEEEECCEEECCCEEEEECCCCCHHHHHHHCCCCEEEEEEE
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7651129; 8969503; 9384377; 7751281 [H]