Click here to switch to the map view.

The map label for this gene is opuCB [H]

Identifier: 225861655

GI number: 225861655

Start: 1690750

End: 1692270

Strand: Reverse

Name: opuCB [H]

Synonym: SPT_1778

Alternate gene names: 225861655

Gene position: 1692270-1690750 (Counterclockwise)

Preceding gene: 225861656

Following gene: 225861654

Centisome position: 80.12

GC content: 43.59

Gene sequence:

>1521_bases
ATGACTAATTTAATTGCAACTTTTCAGGATCGTTTTGGTGATTGGTTGACAGCCCTATCTCAACATTTGCAGTTGTCACT
TTTGACCTTGTTACTAGCTATTTTGCTTGCGATTCCCTTGGCTGTTTATCTTCGCTATCATGAGAAGTTGGCGGATTGGG
TCTTACAGATTGCAGGGATTTTCCAGACCATCCCGTCTCTGGCCTTGTTGGGACTCTTTATTCCCTTGATGGGAATTGGG
ACTTTACCGGCATTGACAGCTCTAGTGATTTATGCGATTTTTCCGATTTTACAAAATACTATTACTGGGCTGAAGGGAAT
TGATCCGAGTCTTCAAGAGGCTGGGATTGCCTTTGGGATGACCAGATGGGAGCGTCTCAAGAAGTTTGAAATTCCACTTG
CCATGCCTGTTATCATGTCTGGAATTCGGACGGCAGCGGTCTTGATTATCGGTACGGCTACCTTGGCGACCTTGATTGGG
GCAGGGGGACTGGGTTCCTTTATCCTGTTGGGAATTGACCGTAATAATGCCAGTTTGATTTTGATTGGGGCCCTTTCTTC
TGCAGTGCTAGCTATTGCCTTTAACTTCCTACTAAAAGTAATGGAAAAAGCGAAATTGCGGACGATTTTCTCTGGTTTTG
CCTTGGTGGCTTTATTACTGGGTCTGTCTTATAGTCCAGCCCTTTTGGCTCAAAAAGAGAAGGAAAACTTGATTATTGCT
GGGAAAATAGGCCCAGAACCAGAAATTTTGGCTAATATGTATAAATTGCTGATTGAAGAAAATACCAGCATGACTGCGAC
TGTTAAACCGAATTTTGGGACGACAAGTTTCCTTTATGAAGCTCTGAAAAAAGGTGATATTGACATTTATCCTGAATTTA
CCGGTACGGTGACTGAAAGTTTGCTTCAACCATCACCCAAGGTGAGTCATGAGCCAGAGCAGGTTTATCAAGTGGCGCGT
GATGGCATTGCCAAGCAGGATCATCTAGCCTATCTCAAACCTATGTCTTATCAAAATACCTATGCTGTAGCTGTTCCGAA
AAAGATTGCTCAAGAATATGGCTTGAAGACCATTTCGGATTTGAAAAAAGTGGAAGGGCAGCTGAAGGCAGGCTTTACGC
TTGAGTTTAACGACCGTGAAGATGGAAATAAGGGCTTGCAATCAATGTATGGTCTCAATCTCAATGTGGCGACTATGCAG
CCAGCTCTTCGTTATCAGGCAATTCAGTCAGGGGATATTCAAATCACGGATGCCTATTCGACTGATGCGGAATTGGAGCG
TTATGATTTACAGGTCTTGGAAGATGACAAGCAACTCTTCCCACCTTATCAAGGGGCTCCACTCATGAAAGAAGCTCTTC
TCAAGAAACACCCAGAGTTGGAAAGAGTTCTTAATACATTGGCTGGTAAGATTACAGAAAGCCAGATGAGCCAGCTCAAC
TACCAAGTCGGTGTTGAAGGCAAGTCAGCAAAGCAAGTAGCCAAGGAGTTTCTCCAAGAACAAGGTTTGTTGAAGAAATG
A

Upstream 100 bases:

>100_bases
GTTTTGCAGGATGGAGAGATTCGTCAGGTGTCGAATCCTGAGACTATTTTAAAAGCTCCTGCAACAGACTTTGTTGCAGA
CTTGTTTGGAGGTAGTGTTC

Downstream 100 bases:

>100_bases
TGGGACAGAGACTTTAAACATACTGAACTCAACTTCCTTAAACGACTAGATAAAAGAATGCTCAGACAATTTCGTCTGGG
CTTTTTTGATGTGAGAATGA

Product: ABC transporter membrane-spanning permease - choline transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYLRYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIG
TLPALTALVIYAIFPILQNTITGLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVALLLGLSYSPALLAQKEKENLIIA
GKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTSFLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVAR
DGIAKQDHLAYLKPMSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGLNLNVATMQ
PALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMKEALLKKHPELERVLNTLAGKITESQMSQLN
YQVGVEGKSAKQVAKEFLQEQGLLKK

Sequences:

>Translated_506_residues
MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYLRYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIG
TLPALTALVIYAIFPILQNTITGLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVALLLGLSYSPALLAQKEKENLIIA
GKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTSFLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVAR
DGIAKQDHLAYLKPMSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGLNLNVATMQ
PALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMKEALLKKHPELERVLNTLAGKITESQMSQLN
YQVGVEGKSAKQVAKEFLQEQGLLKK
>Mature_505_residues
TNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYLRYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGT
LPALTALVIYAIFPILQNTITGLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIGA
GGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVALLLGLSYSPALLAQKEKENLIIAG
KIGPEPEILANMYKLLIEENTSMTATVKPNFGTTSFLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARD
GIAKQDHLAYLKPMSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGLNLNVATMQP
ALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMKEALLKKHPELERVLNTLAGKITESQMSQLNY
QVGVEGKSAKQVAKEFLQEQGLLKK

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1732

COG function: function code M; Periplasmic glycine betaine/choline-binding (lipo)protein of an ABC-type transport system (osmoprotectant binding protein)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788449, Length=185, Percent_Identity=36.7567567567568, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1788452, Length=278, Percent_Identity=26.6187050359712, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788451, Length=203, Percent_Identity=33.9901477832512, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789033, Length=172, Percent_Identity=30.8139534883721, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 55504; Mature: 55373

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYLRYHEKLADWVLQIAGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTITGLKGIDPSLQEAGIAFGM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIGAGGLGSFILLGIDRNNASLI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEE
LIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVALLLGLSYSPALLAQKEKENLIIA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEE
GKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTSFLYEALKKGDIDIYPEFTGTVTES
ECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEECCCCCHHHHHH
LLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKPMSYQNTYAVAVPKKIAQEYGLKTISD
HHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEECCHHHHHHHCCHHHHH
LKKVEGQLKAGFTLEFNDREDGNKGLQSMYGLNLNVATMQPALRYQAIQSGDIQITDAYS
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCHHHHCHHHHHHHHCCCCEEEEECCC
TDAELERYDLQVLEDDKQLFPPYQGAPLMKEALLKKHPELERVLNTLAGKITESQMSQLN
CCCHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
YQVGVEGKSAKQVAKEFLQEQGLLKK
CCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYLRYHEKLADWVLQIAGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTITGLKGIDPSLQEAGIAFGM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIGAGGLGSFILLGIDRNNASLI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEE
LIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVALLLGLSYSPALLAQKEKENLIIA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEE
GKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTSFLYEALKKGDIDIYPEFTGTVTES
ECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEECCCCCHHHHHH
LLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKPMSYQNTYAVAVPKKIAQEYGLKTISD
HHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEECCHHHHHHHCCHHHHH
LKKVEGQLKAGFTLEFNDREDGNKGLQSMYGLNLNVATMQPALRYQAIQSGDIQITDAYS
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCHHHHCHHHHHHHHCCCCEEEEECCC
TDAELERYDLQVLEDDKQLFPPYQGAPLMKEALLKKHPELERVLNTLAGKITESQMSQLN
CCCHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
YQVGVEGKSAKQVAKEFLQEQGLLKK
CCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10216873; 9384377 [H]