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The map label for this gene is aroF [H]

Identifier: 225861524

GI number: 225861524

Start: 1563744

End: 1564775

Strand: Reverse

Name: aroF [H]

Synonym: SPT_1638

Alternate gene names: 225861524

Gene position: 1564775-1563744 (Counterclockwise)

Preceding gene: 225861525

Following gene: 225861523

Centisome position: 74.08

GC content: 44.38

Gene sequence:

>1032_bases
ATGGCATTTATTGAAAAAGGTCAAGAAATCGATATGGAAGTCATTAAGGCTGAAACCCAATTGTCTGCGGAAGCCTTGAG
ACTCAAGGAAAGCCGTGACAGGGAATTGGCAGATATTATTTCAGGGGAAGATGACCGTATTCTCTTGGTGATTGGTCCTT
GCTCTTCTGATAATGAAGAGGCGGTCTTGGAATATGCTCGCCGTTTATCCGCCTTGCAAAAGAAGGTAGCGGACAAGATT
TTCATGGTCATGCGCGTGTATACTGCTAAGCCTCGTACCAATGGAGACGGCTATAAAGGATTAGTTCACCAGCCAGATAC
TTCTAAGGCTCCAAGCCTGATTAATGGCTTGCAGGCTGTGCGCCAGTTGCACTACCGCGTGATTACAGAGACTGGTTTGA
CAACGGCAGATGAGATGCTTTATCCGTCAAATCTGATCTTGGTGGATGACTTGGTCAGCTACCATGCCGTTGGAGCTCGT
TCTGTGGAAGACCAAGAGCACCGCTTTGTGGCTTCTGGGATTGATGCACCAGTAGGGATGAAAAATCCAACCTCAGGAAA
TTTGGGTGTTATGTTTAACGCCATCTATGCTGCTCAAAACAAGCAAACCTTCCTTTATCATGGGCAGGAAGTTGAGACAT
CAGGTAATCCTTTGGCCCATGTTATCCTCCGTGGAGCAGTCAACGAGTATGGCAATTATATGCCGAATTACTACTATGAA
AACCTACTCCAAGCCATTGAACGCTATGAAACCATGGGACTTGAAAATCCTTTTATCCTCATTGACACCAACCATGATAA
CTCAGGCAAGCAATATATGGAGCAGATTCGAATTGTTCGCCAGACCTTGCAGAATCGTGATTGGAATGAGAAAATTAAAA
AGACGGTTCGAGGATTTATGATTGAATCTTACCTAGCAGATGGTCGTCAAAACCAACCAGAGATCTTTGGTTGCTCTATT
ACTGACCCTTGCCTAGGTTGGGAAAATACAGAGGCCTTGGTAGAAGAGATTTATGTTACCTTGACAAAATAA

Upstream 100 bases:

>100_bases
GAGCCAGAAGTATTTGGTAAGTCTATCACAGACCCTTGCCTGGGTTGGGATAACACAGAAGCTCTTGTCAGAGAAATTTA
CAAAACGTTAGGAGAATAAG

Downstream 100 bases:

>100_bases
GTGAAAAGGATGGAGTTGGGGAATCTCAACTCCTTTTGATGAGAATGATAGTTGGACACGGAATTGACATCGAAGAATTG
GCTTCGATAGAAAGCGCAGT

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGEDDRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKI
FMVMRVYTAKPRTNGDGYKGLVHQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPLAHVILRGAVNEYGNYMPNYYYE
NLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQIRIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSI
TDPCLGWENTEALVEEIYVTLTK

Sequences:

>Translated_343_residues
MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGEDDRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKI
FMVMRVYTAKPRTNGDGYKGLVHQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPLAHVILRGAVNEYGNYMPNYYYE
NLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQIRIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSI
TDPCLGWENTEALVEEIYVTLTK
>Mature_342_residues
AFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGEDDRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIF
MVMRVYTAKPRTNGDGYKGLVHQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGARS
VEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPLAHVILRGAVNEYGNYMPNYYYEN
LLQAIERYETMGLENPFILIDTNHDNSGKQYMEQIRIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSIT
DPCLGWENTEALVEEIYVTLTK

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=341, Percent_Identity=39.0029325513196, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1787996, Length=323, Percent_Identity=38.390092879257, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1786969, Length=313, Percent_Identity=38.6581469648562, Blast_Score=199, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320240, Length=321, Percent_Identity=34.5794392523364, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6319726, Length=340, Percent_Identity=35.2941176470588, Blast_Score=181, Evalue=2e-46,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 38753; Mature: 38622

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGEDDRILLVIGPCSSDNEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCCCHH
AVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLVHQPDTSKAPSLINGLQAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGARSVEDQEHRFVASGIDAPVGM
HHHHHHHHHHCCCCCHHHHHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCC
KNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPLAHVILRGAVNEYGNYMPNYYYE
CCCCCCCHHHHHHHHHHCCCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHCCCCHHHHH
NLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQIRIVRQTLQNRDWNEKIKKTVRGFM
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IESYLADGRQNQPEIFGCSITDPCLGWENTEALVEEIYVTLTK
HHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGEDDRILLVIGPCSSDNEE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCCCHH
AVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLVHQPDTSKAPSLINGLQAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGARSVEDQEHRFVASGIDAPVGM
HHHHHHHHHHCCCCCHHHHHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCC
KNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPLAHVILRGAVNEYGNYMPNYYYE
CCCCCCCHHHHHHHHHHCCCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHCCCCHHHHH
NLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQIRIVRQTLQNRDWNEKIKKTVRGFM
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IESYLADGRQNQPEIFGCSITDPCLGWENTEALVEEIYVTLTK
HHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]