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The map label for this gene is ddl [H]

Identifier: 225861499

GI number: 225861499

Start: 1531519

End: 1532562

Strand: Reverse

Name: ddl [H]

Synonym: SPT_1610

Alternate gene names: 225861499

Gene position: 1532562-1531519 (Counterclockwise)

Preceding gene: 225861500

Following gene: 225861498

Centisome position: 72.56

GC content: 44.83

Gene sequence:

>1044_bases
ATGAAACAAACGATTATTCTTTTATATGGTGGACGGAGTGCGGAACGCGAAGTCTCTGTCCTTTCAGCTGAGAGTGTCAT
GCGTGCGGTCAATTACGACCGTTTCACAGTCAAGACTTTCTTTATCAGTCAGTCAGGTGACTTTATCAAAACACAGGAAT
TTAGTCATGCTCCGGGGCAAGAAGACCGTCTCATGACCAATGAAACCATTGATTGGGATAAGAAAGTTGCACCAAGTGCT
ATCTACGAAGAAGGTGCAGTGGTCTTTCCAGTCCTTCACGGGCCAATGGGAGAAGATGGCTCTGTTCAAGGATTCTTGGA
AGTTTTGAAAATGCCTTACGTTGGTTGCAACATTTTGTCATCAAGTCTTGCCATGGATAAAATCACGACTAAGCGTGTTC
TGGAATCTGCTGGTATTGCCCAAGTTCCTTATGTGGCTATCGTTGAAGGCGATGATGTGACTGCTAAAATCGCTGAAGTG
GAAGAAAAATTGGCTTATCCAGTCTTCACTAAGCCGTCAAACATGGGGTCTAGTGTCGGTATTTCTAAGTCTGAAAACCA
AGAAGAACTCCGTCCAGCCTTAGAACTTGCCTTCCGATATGATAGCCGTGTCTTGGTTGAGCAAGGAGTGAATGCCCGTG
AAATTGAGGTTGGCCTCTTGGGTAACTACGATGTCAAGAGCACGCTACCAGGAGAAGTTGTCAAGGATGTTGCCTTTTAT
GACTACGATGCCAAGTATATTGATAACAAGATTACTATGGATATTCCTGCCAAAATCAGTGATGATGTGGTGGCTGTCAT
GCGTCAAAATGCAGAAACAGCCTTCCGTGCCATTGGTGGCCTTGGTCTATCTCGTTGCGATTTCTTCTATACAGATAAGG
GAGAGATTTTTCTCAACGAGCTCAATACCATGCCAGGTTTCACCCAGTGGTCTATGTACCCACTACTTTGGGACAATATG
GGGATCAGCTACCCAGAACTAATCGAGCGTTTGGTTGACCTTGCCAAGGAAAGTTTTGACAAGCGCGAAGCGCATTTGAT
ATAA

Upstream 100 bases:

>100_bases
TTGTATAGTGTGATGAGTAGGATCAGGTTCAAGTTTTAAAAAACCAAGCAAATATGATATACTAAAGAGCGAGTATTCTA
GTAGAATTAGGACAAATAAT

Downstream 100 bases:

>100_bases
AATGAAAGAGAGGGTAGAAGCCAGAACCATCACTGCAAGGTGACTAGAGTTCTCGGACTTCAGCCCTTTTTAAAGGAGTA
GAAATGAAATTAACAATCCA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSA
IYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
EEKLAYPVFTKPSNMGSSVGISKSENQEELRPALELAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFY
DYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNM
GISYPELIERLVDLAKESFDKREAHLI

Sequences:

>Translated_347_residues
MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSA
IYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
EEKLAYPVFTKPSNMGSSVGISKSENQEELRPALELAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFY
DYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNM
GISYPELIERLVDLAKESFDKREAHLI
>Mature_347_residues
MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSA
IYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
EEKLAYPVFTKPSNMGSSVGISKSENQEELRPALELAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFY
DYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNM
GISYPELIERLVDLAKESFDKREAHLI

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=358, Percent_Identity=36.8715083798883, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1786280, Length=344, Percent_Identity=31.9767441860465, Blast_Score=161, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 38687; Mature: 38687

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQSGDFIKTQEFSHAPGQ
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEECCCCCCCCC
EDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILS
CCCEECCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHH
SSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEVEEKLAYPVFTKPSNMGSSVG
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCCCCC
ISKSENQEELRPALELAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFY
CCCCCCHHHHHHHHHHHHHCCCEEHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHC
DYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNE
CCCHHHHCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHCCCHHHCEEEECCCCCEEEEE
LNTMPGFTQWSMYPLLWDNMGISYPELIERLVDLAKESFDKREAHLI
CCCCCCCCCCEECCEEECCCCCCHHHHHHHHHHHHHHHHCHHHCCCC
>Mature Secondary Structure
MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQSGDFIKTQEFSHAPGQ
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEECCCCCCCCC
EDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILS
CCCEECCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHH
SSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEVEEKLAYPVFTKPSNMGSSVG
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCCCCC
ISKSENQEELRPALELAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFY
CCCCCCHHHHHHHHHHHHHCCCEEHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHC
DYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNE
CCCHHHHCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHCCCHHHCEEEECCCCCEEEEE
LNTMPGFTQWSMYPLLWDNMGISYPELIERLVDLAKESFDKREAHLI
CCCCCCCCCCEECCEEECCCCCCHHHHHHHHHHHHHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA