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The map label for this gene is trmD [H]

Identifier: 225861319

GI number: 225861319

Start: 1353432

End: 1354151

Strand: Reverse

Name: trmD [H]

Synonym: SPT_1422

Alternate gene names: 225861319

Gene position: 1354151-1353432 (Counterclockwise)

Preceding gene: 225861320

Following gene: 225861318

Centisome position: 64.11

GC content: 43.06

Gene sequence:

>720_bases
ATGAAGATTGATATTTTAACCCTCTTTCCAGAGATGTTTTCTCCACTGGAGCACTCAATCGTTGGAAAGGCTCGAGAAAA
AGGGCTCTTGGATATCCAGTATCATAATTTTCGAGAAAATGCTGAAAAGGCCCGTCATGTAGATGATGAGCCCTACGGAG
GCGGTCAGGGCATGTTGCTCAGAGCACAACCTATTTTCGATTCCTTTGATGCTATTGAAAAGAAAAATCCGCGCGTTATT
CTCCTCGATCCTGCTGGAAAGCAGTTTGATCAGGCTTATGCTGAAGATTTGGCTCAAGAGGAAGAGCTAATCTTTATCTG
TGGGCACTATGAGGGTTATGATGAGCGCATTAAGACCTTGGTAACAGATGAGATTTCCCTAGGCGACTATGTCCTCACTG
GTGGAGAATTGGCAGCTATGACCATGATTGATGCTACAGTTCGCCTGATTCCAGAAGTGATTGGCAAGGAGTCTAGCCAC
CAAGATGATAGTTTTTCTTCAGGTCTTTTAGAATATCCTCAGTACACACGTCCCTATGATTATCGAGGCATGGTCGTGCC
AGATGTATTGATGAGTGGCCACCATGAAAAGATTCGTCAGTGGCGATTGTACGAGAGTTTAAAGAAAACCTACGAGCGCA
GACCAGATTTACTTGAACATTATCAACTGACAGTAGAAGAAGAAAAAATGCTGGCAGAAATCAAAGAAAACAAAGAATAA

Upstream 100 bases:

>100_bases
GGCAAACGTGATTTGCTTTTACCTTATATCCCACCAGTGGTTCTCAATGTTGATATTCCAAATAAACGGGTCGATGTGGA
AATCTTAGAAGGGTTAGACG

Downstream 100 bases:

>100_bases
AGGAGAAACCTATGCAAGTAATCAAACGTAATGGCGAAATTGCTGAATTTAATCCAGATAAGATTTACCAAGCCATCTTG
AAGGCAGCCCAAACTGTTTA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVI
LLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE

Sequences:

>Translated_239_residues
MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVI
LLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE
>Mature_239_residues
MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVI
LLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=49.3775933609959, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27634; Mature: 27634

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLL
CEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCEEE
RAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTL
EECCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
VTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSHQDDSFSSGLLEYPQYTRPYD
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCC
YRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE
CCCEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLL
CEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCEEE
RAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTL
EECCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
VTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSHQDDSFSSGLLEYPQYTRPYD
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCC
YRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE
CCCEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA