Click here to switch to the map view.

The map label for this gene is ppsA [H]

Identifier: 225861301

GI number: 225861301

Start: 1339460

End: 1340329

Strand: Reverse

Name: ppsA [H]

Synonym: SPT_1404

Alternate gene names: 225861301

Gene position: 1340329-1339460 (Counterclockwise)

Preceding gene: 225861302

Following gene: 225861300

Centisome position: 63.46

GC content: 36.21

Gene sequence:

>870_bases
ATGAGAAATCAACTTGCCCTTAGTGGCGAAAAAATTCTTGAAAAAATTTATCCTCAACTATTTCACCACGTCGGCATGAT
TCGTGGGGAATATTTGTTGAGAGAGCTTAATCAAAACATCATATTAGCAAGTTGTCAGCAATTTGTAAAAGATTATCTTG
ATACTATTTGCTCCTTTTACTTAGATAAGGAAGTTTGGTATCGTTTTTCTGAATTAACGAATACAGAAGCCAATTGTTTA
GCGGGGACTAGAGAGTTTTTTGACGAAGGGCATCCCTTGTTTGGCTATAGAGGAACCAGGCGTTTACTGGCGTGTTTGGA
TGAATTTCAGGCTGAAGCACATGTCGTTACAGAAGTTTATCAAACCAATCCCAATCTGTCTCTTATTTTTCCTTTTGTCA
ATGATGCCGACCAATTAAAACAAGCTATTACAGTATTGCGTCAGTATGGCTTTACTGGGAAAGTCGGAACGATGATTGAA
TTACCGTCAGCGTATTTTGACTTATCTAGTATACTGGAAACGGGCATTTCAAAGATTGTAGTTGGAATGAATGATTTGAC
TTCCTTTGTTTTTGCGACTATGAGAAACAGTCAATGGCATGATATGGAAAGTCCAATAATGTTAGATATGCTAAGAGATA
TGCAGGATAAAGCAAGAAAGAACAAGATTAATTTTGCTGTAGCAGGCTATCTGAATACTTCTTTCATACAAAAAATGAAT
CAGTTGGGTATCAAGTGCATTATCCACTACAGTTCTATTCCAGAGATTTTTGATTTAGAGATTGACCATCCAGATCATCT
TAAACACATAAAAGAAGAAAGTAAAAAATTACAAAGGAGCACCCATGACACCGCAAGAAATGTGGAATAA

Upstream 100 bases:

>100_bases
ACAGAAGAAAATGCATCGCCATTAGTCTTGAAGGTTAAGTCTGAATTATTAGGATTTCCAGAACTGGACAAGACTTCTTA
CATGAATTGGAAGGTGAATG

Downstream 100 bases:

>100_bases
ATACAAACAAATCAATCCTTTGATCGGAGATGAAATCGATGCTTGGGCTTTTGGAGTTGAACCAGACCTTTTAGCGGATT
TGGTGTTTAAAGGAGAAAAG

Product: PEP-utilizing enzyme family protein

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILASCQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCL
AGTREFFDEGHPLFGYRGTRRLLACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKARKNKINFAVAGYLNTSFIQKMN
QLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKKLQRSTHDTARNVE

Sequences:

>Translated_289_residues
MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILASCQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCL
AGTREFFDEGHPLFGYRGTRRLLACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKARKNKINFAVAGYLNTSFIQKMN
QLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKKLQRSTHDTARNVE
>Mature_289_residues
MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILASCQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCL
AGTREFFDEGHPLFGYRGTRRLLACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKARKNKINFAVAGYLNTSFIQKMN
QLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKKLQRSTHDTARNVE

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 33422; Mature: 33422

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILASCQQFVKDYLDTICSFY
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRLLACLDEFQAEAHVVTEVY
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIELPSAYFDLSSILETGISKIV
HCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHEECCHHHHHHHHHHHHHHHHHH
VGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKARKNKINFAVAGYLNTSFIQKMN
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
QLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKKLQRSTHDTARNVE
HCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILASCQQFVKDYLDTICSFY
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRLLACLDEFQAEAHVVTEVY
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIELPSAYFDLSSILETGISKIV
HCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHEECCHHHHHHHHHHHHHHHHHH
VGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKARKNKINFAVAGYLNTSFIQKMN
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
QLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKKLQRSTHDTARNVE
HCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]