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The map label for this gene is ciaR

Identifier: 225861298

GI number: 225861298

Start: 1335593

End: 1336267

Strand: Reverse

Name: ciaR

Synonym: SPT_1401

Alternate gene names: 225861298

Gene position: 1336267-1335593 (Counterclockwise)

Preceding gene: 225861299

Following gene: 225861297

Centisome position: 63.27

GC content: 37.93

Gene sequence:

>675_bases
ATGATAAAAATCTTATTGGTTGAGGATGACCTAGGTCTGTCAAATTCAGTATTTGACTTTTTAGACGATTTTGCGGATGT
TATGCAGGTATTTGATGGAGAAGAAGGTCTCTACGAAGCTGAGAGTGGTGTCTATGACTTGATTTTGCTGGATTTGATGT
TGCCAGAAAAAAATGGTTTCCAAGTCTTAAAAGAATTGCGTGAAAAGGGAATTACGACACCAGTTCTGATTATGACTGCC
AAGGAAAGTTTGGATGACAAGGGACATGGATTTGAACTGGGAGCGGATGATTATCTGACCAAACCTTTCTACCTAGAAGA
ACTTAAAATGCGGATTCAGGCCCTTCTCAAACGTTCAGGGAAGTTTAATGAAAACACCTTGACTTATGGGAATATCGTGG
TTAATTTATCAACCAATACCGTTAAAGTTGAAGATACTCCTGTCGAATTGCTGGGGAAAGAGTTCGATTTACTAGTTTAT
TTCCTTCAAAATCAAAATGTGATTTTGCCTAAGACGCAGATTTTTGACCGTCTATGGGGATTTGATAGTGATACAACGAT
TTCGGTTGTCGAAGTCTATGTTTCAAAAGTCCGTAAGAAATTAAAGGGAACCACTTTTGCAGAGAATTTGCAAACCTTGC
GTAGTGTTGGGTATCTTTTAAAAGATGTTCAGTAA

Upstream 100 bases:

>100_bases
AAAATAAAAAGAAAACTCAGCTATCTCATGTAAAATGCAGAGAGTTGAGTTTTTTTTAAGGCAAATTTGGTATAATAGTT
TTAATAAGGAGGAGTTTCTC

Downstream 100 bases:

>100_bases
ACTTAAAAAAACATGGTATGCGGATGACTTTAGTTATTTTATCCGCAACTTCGGTGTCTTCACCCTGATTTTTTCTACAA
TGACTCTGATTATTTTACAA

Product: transcriptional regulatory protein CiaR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTA
KESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGYLLKDVQ

Sequences:

>Translated_224_residues
MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTA
KESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGYLLKDVQ
>Mature_224_residues
MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTA
KESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGYLLKDVQ

Specific function: Member of the two-component regulatory system CiaH/CiaR. Involved in early steps of competence regulation and in penicillin susceptibility

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=37.8854625550661, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1786784, Length=229, Percent_Identity=34.9344978165939, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI87082012, Length=219, Percent_Identity=36.0730593607306, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=33.7777777777778, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1786599, Length=221, Percent_Identity=31.2217194570136, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=27.1493212669683, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786911, Length=207, Percent_Identity=30.4347826086957, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=30.6306306306306, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=29.3859649122807, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=35.2173913043478, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787229, Length=227, Percent_Identity=29.0748898678414, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790863, Length=226, Percent_Identity=28.3185840707965, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788394, Length=221, Percent_Identity=25.7918552036199, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693140, Length=240, Percent_Identity=28.3333333333333, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790437, Length=126, Percent_Identity=30.1587301587302, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=72, Percent_Identity=38.8888888888889, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): CIAR_STRPN (P0A4H7)

Other databases:

- EMBL:   AE005672
- PIR:   F95092
- RefSeq:   NP_345295.1
- ProteinModelPortal:   P0A4H7
- SMR:   P0A4H7
- EnsemblBacteria:   EBSTRT00000026241
- GeneID:   930748
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_0798
- TIGR:   SP_0798
- GeneTree:   EBGT00050000026872
- HOGENOM:   HBG753323
- OMA:   VILPKEQ
- ProtClustDB:   CLSK876907
- BioCyc:   SPNE170187-1:SP_0798-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00448
- SMART:   SM00862

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 25467; Mature: 25467

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKNGF
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCCH
QVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSG
HHHHHHHHCCCCCCEEEEEEHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
KFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVYFLQNQNVILPKTQIFDRLWG
CCCCCEEEEEEEEEEECCCEEEECCCCHHHHCCHHHEEEEEECCCCEECCHHHHHHHHHC
FDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGYLLKDVQ
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKNGF
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCCH
QVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSG
HHHHHHHHCCCCCCEEEEEEHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
KFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVYFLQNQNVILPKTQIFDRLWG
CCCCCEEEEEEEEEEECCCEEEECCCCHHHHCCHHHEEEEEECCCCEECCHHHHHHHHHC
FDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGYLLKDVQ
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916