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The map label for this gene is hsdS [H]

Identifier: 225861216

GI number: 225861216

Start: 1250015

End: 1251058

Strand: Reverse

Name: hsdS [H]

Synonym: SPT_1309

Alternate gene names: 225861216

Gene position: 1251058-1250015 (Counterclockwise)

Preceding gene: 225861217

Following gene: 225861215

Centisome position: 59.23

GC content: 31.9

Gene sequence:

>1044_bases
ATGAAGAAAGTGAAATTAGGGCAAGTGGCTACTTTTATCAATGGCTATGCTTTTAAACCTCAAGATTGGTCATCTGAAGG
GAAAGAGATTATCAGAATACAGAATCTGACTAAAACTTCAAAAGGGATAAATTATTATTCGGGTACGATTGATAAAAAAT
ACATTGTTGAAGCTGGTGATATTCTTATTTCGTGGTCGGGTACATTAGGGGTTTTTCAGTGGTGTGGAAGATCCGCAGTG
TTAAATCAGCACATTTTTAAAGTGGTTTTTGATAAGATAGATATAGATAAATCGTATTTTAAATATGTTGTTGAAAAAGG
TTTGCAAGATGCTGTAAAACATACTCATGGTTCCACAATGAAACATTTAACTAAAAAATATTTTGATAATATTATGGTTT
CATATACAAATTTGAGAGAGCAACAAAGAATTGCAAGTGAGCTGGATTTGTTAAGCAAGCTGATTTTAAGGAGACAAGAA
CAACTTGAAGAGCTTAATCTACTTGTAAAATCCCGATTTAACGAGATGTTTGGGGATGTTATTCTAAATGAAAAAGAATG
GAAAGTTAGCAAATGGAACGAGATTCTAACAATAAGAAATGGTAAAAATCAAAAACAGGTTGAAGATGCTGATGGGAAAT
TTCCTATTTATGGAAGTGGGGGAATTATGGGATATGCTAAAGATTGGATAGTGAAGAAAAATTCAGTTATTATTGGACGT
AAAGGAAATATAAATAAACCTATTTTAGTTAGAGAAAATTTTTGGAATGTAGATACAGCATTTGGATTAGAGCCTGTTTT
AGAAAAAATAAACTCAGAATATTTATTCTATTTTTGTCAATTATACAATTTTGAGAAGTTAAATAAGGCTGTAACAATAC
CTAGTTTAACCAAATCAGATTTATTAAATATTTCCATCCCCCTCCCCCCACTAGCCCTCCAAAATGAGTTCGCAGACTTT
GTAGCCCTGGTCGACAAATCACAATTTGCTTGTGAGATAGCTATAAAAGTGTGGAGAAATAGCTTGAAATTTAGTATAAT
ATAG

Upstream 100 bases:

>100_bases
AACCCTGGCCTATGCCATGGTCAATCAAAATAATGTCAAGCATTCACACCAAAAATTCATCTCTTAAAAGCAAATCCCGA
TATTCCGAGGAGGTGGTTGC

Downstream 100 bases:

>100_bases
CTAAACTATTTGTTTAAAGTGAGAAAAAAATGGGAAATTTTAGCTTTCTTTTAAAAAATGACGAATATGAATCTTTTTCA
AAACCTTGCATTGAAGCTGA

Product: type I restriction-modification system, S subunit

Products: NA

Alternate protein names: S.EcoBI; Type I restriction enzyme EcoBI specificity protein; S protein [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGINYYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAV
LNQHIFKVVFDKIDIDKSYFKYVVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLREQQRIASELDLLSKLILRRQE
QLEELNLLVKSRFNEMFGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIYGSGGIMGYAKDWIVKKNSVIIGR
KGNINKPILVRENFWNVDTAFGLEPVLEKINSEYLFYFCQLYNFEKLNKAVTIPSLTKSDLLNISIPLPPLALQNEFADF
VALVDKSQFACEIAIKVWRNSLKFSII

Sequences:

>Translated_347_residues
MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGINYYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAV
LNQHIFKVVFDKIDIDKSYFKYVVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLREQQRIASELDLLSKLILRRQE
QLEELNLLVKSRFNEMFGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIYGSGGIMGYAKDWIVKKNSVIIGR
KGNINKPILVRENFWNVDTAFGLEPVLEKINSEYLFYFCQLYNFEKLNKAVTIPSLTKSDLLNISIPLPPLALQNEFADF
VALVDKSQFACEIAIKVWRNSLKFSII
>Mature_347_residues
MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGINYYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAV
LNQHIFKVVFDKIDIDKSYFKYVVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLREQQRIASELDLLSKLILRRQE
QLEELNLLVKSRFNEMFGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIYGSGGIMGYAKDWIVKKNSVIIGR
KGNINKPILVRENFWNVDTAFGLEPVLEKINSEYLFYFCQLYNFEKLNKAVTIPSLTKSDLLNISIPLPPLALQNEFADF
VALVDKSQFACEIAIKVWRNSLKFSII

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 39949; Mature: 39949

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGINYYSGTIDKKYIVEAGD
CCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCEECCCCCCEEEEEECC
ILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKYVVEKGLQDAVKHTHGSTM
EEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHH
KHLTKKYFDNIMVSYTNLREQQRIASELDLLSKLILRRQEQLEELNLLVKSRFNEMFGDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIYGSGGIMGYAKDWIVKKNSVIIGR
HCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCEEEEECCCCCCCHHHHEEECCCEEEEC
KGNINKPILVRENFWNVDTAFGLEPVLEKINSEYLFYFCQLYNFEKLNKAVTIPSLTKSD
CCCCCCCEEEECCCCCCCHHCCHHHHHHHCCCHHHHHHHHHHCHHHHCCEEECCCCCCCC
LLNISIPLPPLALQNEFADFVALVDKSQFACEIAIKVWRNSLKFSII
CEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGINYYSGTIDKKYIVEAGD
CCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCEECCCCCCEEEEEECC
ILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKYVVEKGLQDAVKHTHGSTM
EEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHH
KHLTKKYFDNIMVSYTNLREQQRIASELDLLSKLILRRQEQLEELNLLVKSRFNEMFGDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIYGSGGIMGYAKDWIVKKNSVIIGR
HCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCEEEEECCCCCCCHHHHEEECCCEEEEC
KGNINKPILVRENFWNVDTAFGLEPVLEKINSEYLFYFCQLYNFEKLNKAVTIPSLTKSD
CCCCCCCEEEECCCCCCCHHCCHHHHHHHCCCHHHHHHHHHHCHHHHCCEEECCCCCCCC
LLNISIPLPPLALQNEFADFVALVDKSQFACEIAIKVWRNSLKFSII
CEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6304321 [H]