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The map label for this gene is aguB

Identifier: 225861184

GI number: 225861184

Start: 1212635

End: 1213510

Strand: Reverse

Name: aguB

Synonym: SPT_1277

Alternate gene names: NA

Gene position: 1213510-1212635 (Counterclockwise)

Preceding gene: 225861185

Following gene: 225861182

Centisome position: 57.45

GC content: 43.15

Gene sequence:

>876_bases
ATGAGAAATGTAAGAGTTGCAACCATTCAGATGCAATGCGCTAAGGATGTGGCAACAAATATCCAAACCGCAGAGCGTTT
AGTACGTCAGGCTGCTGAGCAAGGAGCCCAAATTATTCTCTTGCCCGAGTTGTTTGAACATCCCTATTTCTGTCAGGAAC
GTCAGTATGACTACTACCAGTATGCCCAATCTGTAGCGGAAAATACTGCAATTCAGCATTTTAAGGTGATTGCTAAGGAA
CTACAAGTTGTTTTACCAATCAGTTTCTATGAAAAAGATGGTAATGTCTTGTATAACTCTATTGCCGTCATTGATGCAGA
TGGGGAAGTGCTGGGCGTTTATCGAAAGACCCACATACCAGATGACCATTATTATCAAGAAAAATTCTATTTCACGCCTG
GTAACACTGGTTTCAAGGTCTGGAATACTCGCTATGCTAAGATTGGTATCGGTATCTGTTGGGATCAATGGTTCCCTGAA
ACAGCGCGCTGTCTTGCATTGAATGGTGCTGAATTGCTCTTTTATCCTACAGCTATCGGTTCAGAGCCAATTTTGGATAC
AGATAGTTGTGGTCACTGGCAACGTACTATGCAAGGGCACGCAGCAGCGAATATTGTTCCAGTCATCGCAGCCAATCGTT
ATGGTTTAGAGGAGGTTACTCCTAGTGAGGAAAATGGCGGACAGAGTTCCAGTCTTGACTTCTACGGTTCCTCCTTTATG
ACGGATGAAACAGGAGCTATTCTAGAACGAGCTGAAAGACAAGAAGAAGCTGTTCTGTTAGCTACTTATGACCTAGACAA
GGGAGCAAGTGAACGCCTAAACTGGGGCTTGTTTCGAGATAGAAGACCAGAAATGTATAGACAAATTACAGATTAG

Upstream 100 bases:

>100_bases
CCCAGACCGTAAAGTTGTCGGAATACCAGCCAGAGATATTCTCTTAGGTGGTGGCAATATCCACTGTATCACCCAACAAA
TTCCAGAATAGGAGAAAAAG

Downstream 100 bases:

>100_bases
TGTGGGAGAAATGAGAGATTCATTCTGCTAGACTAACTTCTTATTAGCAACTATAAGATACTATGGCATCTAGTAAATCG
ATTTTTATGATTCGCTATTC

Product: N-carbamoylputrescine amidase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKE
LQVVLPISFYEKDGNVLYNSIAVIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEEVTPSEENGGQSSSLDFYGSSFM
TDETGAILERAERQEEAVLLATYDLDKGASERLNWGLFRDRRPEMYRQITD

Sequences:

>Translated_291_residues
MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKE
LQVVLPISFYEKDGNVLYNSIAVIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEEVTPSEENGGQSSSLDFYGSSFM
TDETGAILERAERQEEAVLLATYDLDKGASERLNWGLFRDRRPEMYRQITD
>Mature_291_residues
MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKE
LQVVLPISFYEKDGNVLYNSIAVIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEEVTPSEENGGQSSSLDFYGSSFM
TDETGAILERAERQEEAVLLATYDLDKGASERLNWGLFRDRRPEMYRQITD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=292, Percent_Identity=31.5068493150685, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI7706509, Length=282, Percent_Identity=28.3687943262411, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI5031947, Length=292, Percent_Identity=27.3972602739726, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI297632348, Length=292, Percent_Identity=27.3972602739726, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI297632350, Length=292, Percent_Identity=27.3972602739726, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI297632346, Length=187, Percent_Identity=29.9465240641711, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=307, Percent_Identity=31.5960912052117, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17556280, Length=290, Percent_Identity=23.7931034482759, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323383, Length=299, Percent_Identity=25.4180602006689, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322335, Length=309, Percent_Identity=24.5954692556634, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21358471, Length=304, Percent_Identity=27.9605263157895, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355835, Length=291, Percent_Identity=25.085910652921, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17933642, Length=288, Percent_Identity=25.6944444444444, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33155; Mature: 33155

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPYFCQERQYDYYQ
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCHHHH
YAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIAVIDADGEVLGVYRKTHIP
HHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEEEEEECCCCEEEEEECCCCC
DDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPETARCLALNGAELLFYPTAIG
CCCEECEEEEECCCCCCEEEECEEEEEEEEEEEHHHCCCCCCEEEEECCCEEEEEECCCC
SEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEEVTPSEENGGQSSSLDFYGSSFM
CCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCC
TDETGAILERAERQEEAVLLATYDLDKGASERLNWGLFRDRRPEMYRQITD
CCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPYFCQERQYDYYQ
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCHHHH
YAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIAVIDADGEVLGVYRKTHIP
HHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEEEEEECCCCEEEEEECCCCC
DDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPETARCLALNGAELLFYPTAIG
CCCEECEEEEECCCCCCEEEECEEEEEEEEEEEHHHCCCCCCEEEEECCCEEEEEECCCC
SEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEEVTPSEENGGQSSSLDFYGSSFM
CCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCC
TDETGAILERAERQEEAVLLATYDLDKGASERLNWGLFRDRRPEMYRQITD
CCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]