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The map label for this gene is cysB [H]

Identifier: 225861179

GI number: 225861179

Start: 1209277

End: 1210185

Strand: Reverse

Name: cysB [H]

Synonym: SPT_1272

Alternate gene names: 225861179

Gene position: 1210185-1209277 (Counterclockwise)

Preceding gene: 225861181

Following gene: 225861178

Centisome position: 57.3

GC content: 40.48

Gene sequence:

>909_bases
ATGAACATTCAACAATTACGCTATGTTGTGGCTATTGCCAATAGTGGTACTTTTCGTGAAGCTGCTGAAAAGATGTATGT
TAGTCAGCCGAGTCTGTCTATTTCTGTTCGTGATTTGGAAAAAGAGTTGGGCTTTAAGATTTTCCGTCGGACCAGCTCAG
GGACTTTCTTGACCCGTCGTGGGATGGAATTTTATGAAAAAGCGCAAGAATTGGTTAAAGGATTTGATATTTTTCAAAAT
CAGTATGCCAATCCTGAAGAAGAAAAAGATGAATTTTCCGTTGCTAGCCAGCACTATGACTTCTTACCACCAACTATTAC
GGCCTTTTCAGAGCGCTATCCTGACTATAAGAACTTCCGTATTTTTGAATCAACTACTGTTCAAATATTAGATGAAGTGG
CGCAAGGGCATAGTGAGATTGGGATTATCTACCTCAACAATCAAAATAAAAAGGGGATTATGCAACGGGTTGAAAAGTTA
GGTCTGGAGGTCATCGAATTGATTCCTTTCCATACCCATATTTATCTCTGTGAGGGTCATCCTTTAGCTCAGAAAGAGGA
ATTAGTCATGGAGGATTTAGCGGATTTACCAACGGTTCGTTTCACTCAAGAGAAAGACGAGTACCTTTATTATTCAGAGA
ACTTTGTCGATACCAGCGCTAGCTCACAGATGTTTAATGTGACAGACCGTGCCACCTTGAATGGTATTTTGGAGCGGACG
GACGCCTATGCGACAGGTTCTGGATTTTTAGATAGTGACAGTGTTAATGGCATTACAGTTATTCGTCTCAAGGATAACCT
AGATAACCGCATGGTCTATGTTAAACGTGAAGAAGTGGAGCTTAGTCAAGCTGGGACTCTCTTCGTAGAAGTCATGCAAG
AATATTTTGATCAAAAGAGGAAATCATGA

Upstream 100 bases:

>100_bases
ATAGTGAAGATAGTTAAGAAGAATAGGATGTTCTTTTTTCCTTTTTGGAAAACTTCTAAAATATGGTATAATGAAAAGAT
AAAGAAGTTGGGGGTAGAAG

Downstream 100 bases:

>100_bases
AAAAAAGAGCAATAGTGGCAGTCATTGTACTGCTTTTAATTGGGCTGGATCAGTTGGTCAAATCCTATATCGTCCAGCAG
ATTCCACTGGGTGAAGTGCG

Product: regulatory function on capsule expression in group B streptococci

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQN
QYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERT
DAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKRKS

Sequences:

>Translated_302_residues
MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQN
QYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERT
DAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKRKS
>Mature_302_residues
MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQN
QYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERT
DAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKRKS

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=212, Percent_Identity=31.1320754716981, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790399, Length=204, Percent_Identity=27.9411764705882, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693105, Length=300, Percent_Identity=24, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787601, Length=141, Percent_Identity=31.9148936170213, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=199, Percent_Identity=28.643216080402, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788887, Length=196, Percent_Identity=26.530612244898, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI157672245, Length=307, Percent_Identity=23.7785016286645, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34908; Mature: 34908

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRR
CCHHHEEEEEEEECCCCHHHHHHHHHHCCCCCEEEHHHHHHHHCHHEEEECCCCCEEECC
GMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFR
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEE
IFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKLGLEVIELIPFHTHIYLCEGH
EEHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
PLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERT
CCCHHHHHHHHHHHCCCCEEEECCCCCEEEEECCEECCCCCCCEEECCHHHHHHHHHHHH
DAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKR
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHC
KS
CC
>Mature Secondary Structure
MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRR
CCHHHEEEEEEEECCCCHHHHHHHHHHCCCCCEEEHHHHHHHHCHHEEEECCCCCEEECC
GMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFR
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEE
IFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKLGLEVIELIPFHTHIYLCEGH
EEHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
PLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERT
CCCHHHHHHHHHHHCCCCEEEECCCCCEEEEECCEECCCCCCCEEECCHHHHHHHHHHHH
DAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKR
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]