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The map label for this gene is acoA [H]

Identifier: 225861104

GI number: 225861104

Start: 1137132

End: 1138100

Strand: Reverse

Name: acoA [H]

Synonym: SPT_1190

Alternate gene names: 225861104

Gene position: 1138100-1137132 (Counterclockwise)

Preceding gene: 225861105

Following gene: 225861103

Centisome position: 53.88

GC content: 43.65

Gene sequence:

>969_bases
ATGTCAACTTTAGATAAAAATCTTTTGCTAGAAATGTTCCGTAAGATGGAAGAAATCCGTCGCATGGACTTAAAAATTGC
ACAATTAGTAAAGAAAGGGAAAGTGCCAGGAATGACGCACTTTTCTGTTGGTGAAGAGGCAGCTAACGTGGGGGCTATGT
TAGCTCTCAATCCAGATGATCTGATTACCTCAAACCACCGTGGACACGGGCAAGCTATTGCTAAAGGGATTGACCTCAAC
GGAATGATGGCTGAAATCCTTGGTAAATACACTGGAACCTGTAAAGGGAAAGGTGGATCTATGCATATCGCTGACCTTGA
TGCTGGGAACCTTGGTGCCAATGGTATCGTAGGTGGTGGTATGGGGATCGCTGTCGGTGCAGCCCTCAGTCAGCAAATGC
AAAATACCGGTAAAATCGTTGTCTGCTTCTTTGGAGATGGTGCGACCAATGAAGGTGTTTTCCACGAAGCAGTGAACATG
GCTTCTATCTGGAACCTGCCAGTCATTTTCTATTGCATTAACAACGGTTACGGTATCTCTGCGGATATCAAGAAAATGAC
CAATATAGAACATATCCATCAACGTAGCGCCGCTTATGGAATTCCTGGAATGTTCATCGAAGACGGTAACAATGTCATCG
ATGTCTATGAAGGATTTCAGAAAGCTGTAGACCATGTTCGCAGTGGCAATGGTCCAGTCTTGATTGAAAGTGTAACTTAT
CGCTGGCTCGGTCACTCATCATCTGACCCTGGTAAATATCGTACGCGTGAAGAAGTGGAATTGTGGAAACAAAAAGATCC
AATCGAAAACCTCCGCAATTACCTTATTGAAAATAACATTGCAAGTGCCGAAGAATTGGAAGAAATCCAAGCGCAAGTAA
AGGAAGCAGTAGAAGCTTCTGTTAAATTTGCAGAGGAAAGCCCATTCCCACCGCTTGAATCAGCCTTTGAAGATATTTAC
GCAGACTAA

Upstream 100 bases:

>100_bases
TTCTTTTTTATTTTGTGAAATTTCTTTTAGAAAAACTTTGACAGTATTTGCCAAAAGCGGTAAAATAGTAAGGTAAGAAG
AAAGTTAGAAAGGCAAGAAG

Downstream 100 bases:

>100_bases
GGAGAAAGAGATAAAATGGAAACAAAAACAATGTCCTTCCGTGACACCATTATCCTTGCTATGTCTGAGGAAATGCGTCG
CGATGAAAATGTGTTCTTGA

Product: pyruvate dehydrogenase E1 component subunit alpha

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVGEEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLN
GMMAEILGKYTGTCKGKGGSMHIADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEGFQKAVDHVRSGNGPVLIESVTY
RWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLIENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIY
AD

Sequences:

>Translated_322_residues
MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVGEEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLN
GMMAEILGKYTGTCKGKGGSMHIADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEGFQKAVDHVRSGNGPVLIESVTY
RWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLIENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIY
AD
>Mature_321_residues
STLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVGEEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNG
MMAEILGKYTGTCKGKGGSMHIADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNMA
SIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEGFQKAVDHVRSGNGPVLIESVTYR
WLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLIENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYA
D

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=314, Percent_Identity=38.5350318471338, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI291084742, Length=315, Percent_Identity=39.6825396825397, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI4505685, Length=315, Percent_Identity=39.6825396825397, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI291084744, Length=322, Percent_Identity=38.8198757763975, Blast_Score=211, Evalue=5e-55,
Organism=Homo sapiens, GI291084757, Length=315, Percent_Identity=37.1428571428571, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI11386135, Length=322, Percent_Identity=31.3664596273292, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI258645172, Length=322, Percent_Identity=31.3664596273292, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI32564172, Length=320, Percent_Identity=36.25, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17536047, Length=320, Percent_Identity=36.25, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI86563355, Length=321, Percent_Identity=32.7102803738318, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI86563357, Length=321, Percent_Identity=32.7102803738318, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=37.6175548589342, Blast_Score=217, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24639748, Length=320, Percent_Identity=36.25, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24639740, Length=322, Percent_Identity=34.7826086956522, Blast_Score=197, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24639744, Length=322, Percent_Identity=34.7826086956522, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI28571106, Length=322, Percent_Identity=34.7826086956522, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24639746, Length=310, Percent_Identity=35.4838709677419, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI21355903, Length=324, Percent_Identity=29.6296296296296, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35081; Mature: 34950

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVGEEAANVGAMLALNPDD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHCCCEEEEECCHH
LITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMHIADLDAGNLGANGIVGGG
HHCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCC
MGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNMASIWNLPVIFYCINNGYGIS
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
ADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEGFQKAVDHVRSGNGPVLIESVTY
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEH
RWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLIENNIASAEELEEIQAQVKEAVEAS
HCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VKFAEESPFPPLESAFEDIYAD
HHHHCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
STLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVGEEAANVGAMLALNPDD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHCCCEEEEECCHH
LITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMHIADLDAGNLGANGIVGGG
HHCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCC
MGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNMASIWNLPVIFYCINNGYGIS
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
ADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEGFQKAVDHVRSGNGPVLIESVTY
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEH
RWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLIENNIASAEELEEIQAQVKEAVEAS
HCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VKFAEESPFPPLESAFEDIYAD
HHHHCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]