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The map label for this gene is gdh [H]

Identifier: 225860866

GI number: 225860866

Start: 873569

End: 874915

Strand: Reverse

Name: gdh [H]

Synonym: SPT_0923

Alternate gene names: 225860866

Gene position: 874915-873569 (Counterclockwise)

Preceding gene: 225860870

Following gene: 225860837

Centisome position: 41.42

GC content: 42.69

Gene sequence:

>1347_bases
ATGACATCTGCTAAAGAATATATCCAAAGCGTGTTTGAAACTGTGAAAGATCGTAACGGGCACGAGGCTGAATTCCTCCA
AGCTGTTGAAGAATTTTTCAACACTTTAGAACCTGTATTTGAAAAACACCCTGAGTATATCGAAGAAAATATCTTGGCAC
GTATTACTGAGCCTGAGCGCGTGGTTTCTTTCCGTGTTCCTTGGGTTGACCGTGATGGAAAAATTCAAGTAAACCGTGGT
TACCGTGTTCAATTCAACTCAGCTGTTGGACCATACAAAGGTGGACTTCGTTTCCACCCAACTGTAAACCAAGGGATCTT
GAAATTCCTCGGATTTGAACAAATCTTTAAAAATGTCTTGACTGGACTTCCTATCGGTGGAGGTAAAGGTGGATCAGACT
TCAATCCTAAAGGTAAAACAGATGCTGAAGTGATGCGCTTCTGCCAAAGCTTCATGACTGAATTGCAAAAACACATCGGA
CCATCACTTGACGTACCTGCTGGTGATATCGGTGTTGGTGGACGTGAGATTGGTTACCTTTACGGTCAATACAAACGTCT
TAACCAATTTGATGCTGGTGTCTTGACTGGTAAACCTCTTGGATTTGGTGGTAGCTTGATTCGTCCAGAAGCAACTGGTT
ACGGTTTGGTTTACTATACTGAAGAAATGCTCAAAGCTAACGGTAACAGCTTTGCTGGTAAGAAAGTCGTTATTTCAGGT
TCTGGTAACGTTGCTCAATATGCTCTTCAAAAAGCAACTGAACTCGGTGCAACTGTTATTTCTGTTTCTGACTCAAATGG
TTATGTCATCGATGAAAATGGTATCGACTTCGATCTTTTGGTTGATGTTAAAGAAAAACGTCGTGCTCGTTTGACTGAGT
ATGCAGCTGAAAAAGCAACTGCAACCTACCACGAAGGTACTGTATGGACTTACGCTGGAAACTATGACATTGCTCTTCCA
TGTGCCACTCAAAACGAAATCAACGGTGAAGCAGCTAAACGTTTGGTTGCTCAAGGCGTTATCTGTGTATCTGAAGGTGC
CAACATGCCAAGCGACCTTGATGCCATCAAAGTTTACAAAGAAAATGGTATCTTCTACGGACCTGCAAAAGCTGCCAACG
CTGGTGGTGTAGCCGTTTCAGCCCTTGAAATGAGCCAAAACAGCCTTCGCCTCTCATGGACTCGTGAAGAAGTTGATGGA
CGTCTCAAAGACATCATGACCAACATCTTTAACACAGCTAAAACAACTTCAGAAACATATGGTCTTGATAAAGACTACCT
TGCAGGAGCTAACATTGCTGCCTTTGAAAATGTAGCAAACGCTATGATTGCCCAAGGCGTGGTATAA

Upstream 100 bases:

>100_bases
TGAAAGAATTTTTAGAAAATTTCTGTTTTTTCTTTACAGTGAAAAAAAATTCTGCTATAATGTCTTATGTAATAAAATAT
GACAATAAAAGGAGAACGAT

Downstream 100 bases:

>100_bases
AAAAAAATTACGGAAACTGACTACCCAGACGGGTAGCCAGTTTTTTCAATTCCTAAAATTTAAGATTCTAATACCCTTTA
TGCAAAAATATAGAATTCTA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTSAKEYIQSVFETVKDRNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPERVVSFRVPWVDRDGKIQVNRG
YRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFNPKGKTDAEVMRFCQSFMTELQKHIG
PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALP
CATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDG
RLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV

Sequences:

>Translated_448_residues
MTSAKEYIQSVFETVKDRNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPERVVSFRVPWVDRDGKIQVNRG
YRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFNPKGKTDAEVMRFCQSFMTELQKHIG
PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALP
CATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDG
RLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV
>Mature_447_residues
TSAKEYIQSVFETVKDRNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGY
RVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFNPKGKTDAEVMRFCQSFMTELQKHIGP
SLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISGS
GNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPC
ATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGR
LKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI4885281, Length=410, Percent_Identity=30.4878048780488, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI31377775, Length=410, Percent_Identity=30.4878048780488, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1788059, Length=444, Percent_Identity=55.1801801801802, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17544676, Length=360, Percent_Identity=29.1666666666667, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319256, Length=455, Percent_Identity=52.967032967033, Blast_Score=428, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6324951, Length=453, Percent_Identity=52.9801324503311, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24649283, Length=401, Percent_Identity=29.925187032419, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45549226, Length=359, Percent_Identity=31.7548746518106, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI45553475, Length=359, Percent_Identity=31.7548746518106, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24649460, Length=294, Percent_Identity=32.9931972789116, Blast_Score=121, Evalue=9e-28,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48841; Mature: 48709

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAKEYIQSVFETVKDRNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPER
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCC
VVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVL
EEEEECCEECCCCEEEECCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHHH
TGLPIGGGKGGSDFNPKGKTDAEVMRFCQSFMTELQKHIGPSLDVPAGDIGVGGREIGYL
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
YGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
HHHHHHHHHCCCCEECCCCCCCCCCEECCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEC
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKAT
CCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHC
ATYHEGTVWTYAGNYDIALPCATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYK
CEEECCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCHHHEEEE
ENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGRLKDIMTNIFNTAKTTSETY
CCCEEECCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHC
GLDKDYLAGANIAAFENVANAMIAQGVV
CCCHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSAKEYIQSVFETVKDRNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPER
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCC
VVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVL
EEEEECCEECCCCEEEECCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHHH
TGLPIGGGKGGSDFNPKGKTDAEVMRFCQSFMTELQKHIGPSLDVPAGDIGVGGREIGYL
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
YGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
HHHHHHHHHCCCCEECCCCCCCCCCEECCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEC
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKAT
CCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHC
ATYHEGTVWTYAGNYDIALPCATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYK
CEEECCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCHHHEEEE
ENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGRLKDIMTNIFNTAKTTSETY
CCCEEECCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHC
GLDKDYLAGANIAAFENVANAMIAQGVV
CCCHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]