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The map label for this gene is agaR [H]

Identifier: 225859933

GI number: 225859933

Start: 2100382

End: 2102598

Strand: Reverse

Name: agaR [H]

Synonym: SP70585_2287

Alternate gene names: 225859933

Gene position: 2102598-2100382 (Counterclockwise)

Preceding gene: 225859934

Following gene: 225859932

Centisome position: 96.24

GC content: 32.84

Gene sequence:

>2217_bases
ATGACGATTTATATTAATAAGGACGAGACCGTTTTTCATTTGGCAATGAAAGATAGTAGTTATATTTTTAGAATTTTAGA
AAATGGGGAACTTCAACATCTACATTTTGGGAAAAGGATTCATGTCAAGGAAAATTATAACCAATTGATGGCCTATGAAA
AAAGAGGATTTGAAGTATCTTTTTCTGAAGAATTTGAGGATATTCAACAGTCTATGATACAAAATGAATATTCTTCATAT
GGGAAAGGAGATTTTCGGCATCCAGCCTTTCAAGTTCAAGGAATGAATGGTAGTAGGATAACGACACTAAAATATCAAGG
TTTTGAACTTGAAAAAGGGAAAAATCGTCTTAACTCTCTACCTTCAACATTTGATGATATTGGTCAGTGTGCGGAAACAT
TAACGATTATTTTAACAGATTCCATATTATATTTAACTGTTAGACTAAATTACACAATTTTTCCGGAATACAATGTCTTA
GTTAGAAATACGGAATTTTTAAATAATAGCAATAATAAGTTGACTCTTTTGAAAGCAATGAGCTTACAGCTAGATCTACC
TGATAGTCAATATGACTTTATTCAATTTTCTGGAGCATGGCTGAGGGAACGTCAGTTATATAGAACTTCGCTTAGACCAG
GTATTCAAGCAATAGATAGCTTGAGATACTCATCAAGTCCTCAGCAAAATCCTTTCTTTATGCTATCAAGGAGGGAAACT
ACAGAGCATAGTGGTGAGGTTTATGGTTTTAACTTTATCTATTCTGGAAATTTTCAAAATATGATTGAAGTTGACCATTT
TGACACCGCTAGAGTAACGGTAGGAATAAATCCAGTAGAATTTCGTTTTTTATTAAATCCTGCCGAAAGTTTTGTGACAC
CAGAAGCAATTGTGATCTATTCTGATCAAGGGATGAATCAGATGAGCCAACAACTATCAGATTTTTATCGACATCATTTA
GTTAATCCTAATTTTTCTCAAGCTAGTCGTCCTATAATACTCAATAGTTGGGAAACATTTTATTTTGACTTGAGTACAGA
AAAAATTTTAGATTTAGCAAAGGCTGCTAAAGATTTAGGGATAGAATTATTTGTACTGGATGATGGTTGGTTTGGTCATA
GGAAAGATGACAAAAGTTCTCTGGGTGATTGGGTAACAGATAGAAGTCGCCTTCCTGAAGGTATTGGATTTCTTGCAGAT
GAAATTCACAAAATAGGTTTACAATTTGGTTTGTGGTTTGAGCCTGAAATGATTTCTATTGATAGTGATTTGTACAAGAA
TCATGCCGATTGGACTATCCATTTGTTAGACAGAGAGAAGTCAGTAGGAAGAAATCAATATGTGTTGGATTTGACGAGAC
AGGAAGTTGTTGATTATCTTTTTGATTCTATTTCTAAAATCATAATCAAAACAAATCTGGATTATATCAAATGGGATATG
AATCGTCATATAACAGATATTTATAGTATTGAACTTGATTCTGAACAGCAGATGGAATTTGGTCATCGATATATCTTAGG
TCTTTATCAGTTATTAGATCGTTTAATAACTAAGTTCCCTTCAGTTCTATTTGAATCTTGTTCTTCAGGTGGTGGACGTT
TTGATTTAGGACTTATGTATTATGCACCGCAAGCGTGGACGAGTGATGATACGGACCCGATAGAAAGATTGAAAATTCAG
CATGGAACTTCTTATGGATATTCTCCATCAATGATGACAGCCCATGTTTCTATTTCTCCAAATGAACAAAGTGGAAGACA
AACGAGTTTGGACACTAGGACAAATGTAGCTTATTTTAGTTCTTTCGGTTATGAATTAGATGTTACGAGATTGTCGGTAG
AAGAAAAAGAACAAGTGAGAGAACAAATTCAGTTTTATAAAAAATATCGTTCATTGCTTCAATATGGGGATTTCTATAGG
ATAAACAGTCCTTTTAGTTGTGATTCTGCTAGTTGGCAAGTTGTTTCAAAAGATAAATGCCAATCGATTTTATTGTATGC
TCAATTGAATAGTAAGTTGAATCCAGGTTATACAAGAGTTTATTTTAGTGGTTTAGATAAAGATAAATGCTATTCCGTCT
CTGGATTTGATGAGTTCTTTTATGGAGATGAATTAATGAATGCTGGAATAAAAGTAAGTTTAAGTAATTTAGCACTTTGT
GTTCCAGAATATCTTACAAAATTATTTGTTATTGAAGAAGTTGTATGTAAATATTGA

Upstream 100 bases:

>100_bases
CAGAGTAATTGGAGATTTTACTATTCAAGAAGATGAGATAGAATTGGCACCGTACTCTATCGTGATACTGAAGAAGTAAA
GAAAAAATTGTGAGGTAGAG

Downstream 100 bases:

>100_bases
GGAAATAAAAATGGAAAGTAAAAAAATAGCAAAACAGATTTTAATAGCTACGGCTGTTTTAACAAGTTTTTTAGGAAGTA
ATTTGGTTTATGCAGATGTA

Product: alpha-galactosidase AgaN

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKENYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSY
GKGDFRHPAFQVQGMNGSRITTLKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILYLTVRLNYTIFPEYNVL
VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAIDSLRYSSSPQQNPFFMLSRRET
TEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGINPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHL
VNPNFSQASRPIILNSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLPEGIGFLAD
EIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLDLTRQEVVDYLFDSISKIIIKTNLDYIKWDM
NRHITDIYSIELDSEQQMEFGHRYILGLYQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQ
HGTSYGYSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQFYKKYRSLLQYGDFYR
INSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYFSGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLALC
VPEYLTKLFVIEEVVCKY

Sequences:

>Translated_738_residues
MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKENYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSY
GKGDFRHPAFQVQGMNGSRITTLKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILYLTVRLNYTIFPEYNVL
VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAIDSLRYSSSPQQNPFFMLSRRET
TEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGINPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHL
VNPNFSQASRPIILNSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLPEGIGFLAD
EIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLDLTRQEVVDYLFDSISKIIIKTNLDYIKWDM
NRHITDIYSIELDSEQQMEFGHRYILGLYQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQ
HGTSYGYSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQFYKKYRSLLQYGDFYR
INSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYFSGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLALC
VPEYLTKLFVIEEVVCKY
>Mature_737_residues
TIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKENYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYG
KGDFRHPAFQVQGMNGSRITTLKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILYLTVRLNYTIFPEYNVLV
RNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAIDSLRYSSSPQQNPFFMLSRRETT
EHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGINPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLV
NPNFSQASRPIILNSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLPEGIGFLADE
IHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLDLTRQEVVDYLFDSISKIIIKTNLDYIKWDMN
RHITDIYSIELDSEQQMEFGHRYILGLYQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQH
GTSYGYSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQFYKKYRSLLQYGDFYRI
NSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYFSGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLALCV
PEYLTKLFVIEEVVCKY

Specific function: Alpha-galactosidase associated with the raffinose operon [H]

COG id: COG3345

COG function: function code G; Alpha-galactosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 36 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002252
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 85672; Mature: 85540

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKENYNQLMAYEKRGFEVS
CEEEEECCCEEEEEEECCCCEEEEEECCCCEEEEECCCEEEEECCHHHHHHHHHCCCEEE
FSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITTLKYQGFELEKGKNRLNSL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECCEECHHHHHHHHCC
PSTFDDIGQCAETLTIILTDSILYLTVRLNYTIFPEYNVLVRNTEFLNNSNNKLTLLKAM
CCHHHHHHHHHHHEEEEEECCEEEEEEEEEEEECCCCEEEEEEEHHHCCCCCEEEEEEEE
SLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAIDSLRYSSSPQQNPFFMLSRRET
EEEEECCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCC
TEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGINPVEFRFLLNPAESFVTPEAIVIY
CCCCCCEEEEEEEEECCCCCEEEECCCCCEEEEEECCCEEEEEEECCHHHCCCCCEEEEE
SDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIILNSWETFYFDLSTEKILDLAKAAKDLG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEECCHHHHHHHHHHHHHCC
IELFVLDDGWFGHRKDDKSSLGDWVTDRSRLPEGIGFLADEIHKIGLQFGLWFEPEMISI
EEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCCEEEC
DSDLYKNHADWTIHLLDREKSVGRNQYVLDLTRQEVVDYLFDSISKIIIKTNLDYIKWDM
CHHHHCCCCCEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHEEEECCCCEEEEEC
NRHITDIYSIELDSEQQMEFGHRYILGLYQLLDRLITKFPSVLFESCSSGGGRFDLGLMY
CCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
YAPQAWTSDDTDPIERLKIQHGTSYGYSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFS
ECCCCCCCCCCCHHHHHEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEE
SFGYELDVTRLSVEEKEQVREQIQFYKKYRSLLQYGDFYRINSPFSCDSASWQVVSKDKC
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEHHCH
QSILLYAQLNSKLNPGYTRVYFSGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLALC
HHEEEEEEECCCCCCCEEEEEEECCCCCCCEECCCCHHHHCCHHHHCCCCEEEHHHHHHH
VPEYLTKLFVIEEVVCKY
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKENYNQLMAYEKRGFEVS
EEEEECCCEEEEEEECCCCEEEEEECCCCEEEEECCCEEEEECCHHHHHHHHHCCCEEE
FSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITTLKYQGFELEKGKNRLNSL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECCEECHHHHHHHHCC
PSTFDDIGQCAETLTIILTDSILYLTVRLNYTIFPEYNVLVRNTEFLNNSNNKLTLLKAM
CCHHHHHHHHHHHEEEEEECCEEEEEEEEEEEECCCCEEEEEEEHHHCCCCCEEEEEEEE
SLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAIDSLRYSSSPQQNPFFMLSRRET
EEEEECCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCC
TEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGINPVEFRFLLNPAESFVTPEAIVIY
CCCCCCEEEEEEEEECCCCCEEEECCCCCEEEEEECCCEEEEEEECCHHHCCCCCEEEEE
SDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIILNSWETFYFDLSTEKILDLAKAAKDLG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEECCHHHHHHHHHHHHHCC
IELFVLDDGWFGHRKDDKSSLGDWVTDRSRLPEGIGFLADEIHKIGLQFGLWFEPEMISI
EEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCCEEEC
DSDLYKNHADWTIHLLDREKSVGRNQYVLDLTRQEVVDYLFDSISKIIIKTNLDYIKWDM
CHHHHCCCCCEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHEEEECCCCEEEEEC
NRHITDIYSIELDSEQQMEFGHRYILGLYQLLDRLITKFPSVLFESCSSGGGRFDLGLMY
CCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
YAPQAWTSDDTDPIERLKIQHGTSYGYSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFS
ECCCCCCCCCCCHHHHHEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEE
SFGYELDVTRLSVEEKEQVREQIQFYKKYRSLLQYGDFYRINSPFSCDSASWQVVSKDKC
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEHHCH
QSILLYAQLNSKLNPGYTRVYFSGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLALC
HHEEEEEEECCCCCCCEEEEEEECCCCCCCEECCCCHHHHCCHHHHCCCCEEEHHHHHHH
VPEYLTKLFVIEEVVCKY
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA