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The map label for this gene is kdgA [H]

Identifier: 225859886

GI number: 225859886

Start: 2049057

End: 2049677

Strand: Reverse

Name: kdgA [H]

Synonym: SP70585_2239

Alternate gene names: 225859886

Gene position: 2049677-2049057 (Counterclockwise)

Preceding gene: 225859887

Following gene: 225859885

Centisome position: 93.82

GC content: 44.12

Gene sequence:

>621_bases
ATGTTAAGACAATTAAAAGAAAATTACTTTTTCGCAGTGATTCGCGGAAAAGATGAACAAGATGCAAAAGAAATTGCTCG
TCATGCGGTTTTAGGTGGTATTCGAAATATCGAGATTACATTTTCGACGCCTAATGCTGCGACAGTCATCAAAGAATTAC
AAGAAGAGTTCTCAGATGATTCGTCTGTAGTCATCGGTGCAGGCACAGTAATGAATCTTAATCTCGCACAGGAGGCGATT
GGTGCAGGGGCTAGTTTTCTTGTTAGTCCTCATTTTGATAAGAAAATCCAAGAGTTAGCGCAAGAGACAGGGGTTCTCTA
TTTTCCTGGTTGTGCAACTGCAACGGAAATTGTTACAGCTAGTCAGGCTGGGTGTTCTATTATCAAGCTTTTCCCTGGTG
GTGTATTGGGACATGGGTTTATCAAAGATATTCACGGTCCCGTCCCTGAAGTGGACTTGATGCCATCTGGTGGAGTATCT
GTTGACAATGTTGCGGATTGGAAAAAGGCCGGTGCTTGTGCTGTTGGAATTGGTTCGGCATTGGCGAGTCGGGTGCAGGC
TGAGGGCTATGACAGTGTAACGTTGATTGCTCGTAGTTTTGTAGCAGCTTTGGAGGGATAA

Upstream 100 bases:

>100_bases
AAGAGTCTTCTGGGTATTTTTTTGCTTTTTCCTTGACAAATCATCTGATGAATTATATACTATAACTATAAATAGCAACG
CTTTCTAATTGGAGGTTAAG

Downstream 100 bases:

>100_bases
GATGAGTTTTAACGATAAAAACTTTATGCTAAAAAATGAACCTGCTAAGGAGCTTTACGTGAAAATAGCAGAGCTACCTA
TCTATGATTTTCATTGTCAC

Product: khg/kdpg aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MLRQLKENYFFAVIRGKDEQDAKEIARHAVLGGIRNIEITFSTPNAATVIKELQEEFSDDSSVVIGAGTVMNLNLAQEAI
GAGASFLVSPHFDKKIQELAQETGVLYFPGCATATEIVTASQAGCSIIKLFPGGVLGHGFIKDIHGPVPEVDLMPSGGVS
VDNVADWKKAGACAVGIGSALASRVQAEGYDSVTLIARSFVAALEG

Sequences:

>Translated_206_residues
MLRQLKENYFFAVIRGKDEQDAKEIARHAVLGGIRNIEITFSTPNAATVIKELQEEFSDDSSVVIGAGTVMNLNLAQEAI
GAGASFLVSPHFDKKIQELAQETGVLYFPGCATATEIVTASQAGCSIIKLFPGGVLGHGFIKDIHGPVPEVDLMPSGGVS
VDNVADWKKAGACAVGIGSALASRVQAEGYDSVTLIARSFVAALEG
>Mature_206_residues
MLRQLKENYFFAVIRGKDEQDAKEIARHAVLGGIRNIEITFSTPNAATVIKELQEEFSDDSSVVIGAGTVMNLNLAQEAI
GAGASFLVSPHFDKKIQELAQETGVLYFPGCATATEIVTASQAGCSIIKLFPGGVLGHGFIKDIHGPVPEVDLMPSGGVS
VDNVADWKKAGACAVGIGSALASRVQAEGYDSVTLIARSFVAALEG

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=173, Percent_Identity=30.0578034682081, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1788156, Length=188, Percent_Identity=31.3829787234043, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21608; Mature: 21608

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQLKENYFFAVIRGKDEQDAKEIARHAVLGGIRNIEITFSTPNAATVIKELQEEFSDD
CCCHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCC
SSVVIGAGTVMNLNLAQEAIGAGASFLVSPHFDKKIQELAQETGVLYFPGCATATEIVTA
CCEEEECCEEEEHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCEEEECCCCCHHHHHHH
SQAGCSIIKLFPGGVLGHGFIKDIHGPVPEVDLMPSGGVSVDNVADWKKAGACAVGIGSA
CCCCCEEEEECCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHCCCCEEHHHHH
LASRVQAEGYDSVTLIARSFVAALEG
HHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRQLKENYFFAVIRGKDEQDAKEIARHAVLGGIRNIEITFSTPNAATVIKELQEEFSDD
CCCHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCC
SSVVIGAGTVMNLNLAQEAIGAGASFLVSPHFDKKIQELAQETGVLYFPGCATATEIVTA
CCEEEECCEEEEHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCEEEECCCCCHHHHHHH
SQAGCSIIKLFPGGVLGHGFIKDIHGPVPEVDLMPSGGVSVDNVADWKKAGACAVGIGSA
CCCCCEEEEECCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHCCCCEEHHHHH
LASRVQAEGYDSVTLIARSFVAALEG
HHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]