Click here to switch to the map view.

The map label for this gene is ysgA [H]

Identifier: 225859729

GI number: 225859729

Start: 1883534

End: 1884274

Strand: Reverse

Name: ysgA [H]

Synonym: SP70585_2045

Alternate gene names: 225859729

Gene position: 1884274-1883534 (Counterclockwise)

Preceding gene: 225859732

Following gene: 225859728

Centisome position: 86.25

GC content: 41.43

Gene sequence:

>741_bases
ATGACTATTATAACCTCAAAAGCCAATTCTGTGGTAAAAAATGCTAAGAAATTACACCAAAAAAAATACCGCAAGTCTGC
CTATTTGATTGAAGGCTGGCACTTGTTTGAAGAAGCTGTCCAAGCTGGAGTGACGATTGAGAAAATCTTTGCCCTAGAAA
ATTATCGAAATCAGTTAGCTGCTTTTCCGCAAACTGTCTGGATTTCAGAGGATATTTTGCTAGATTTGGCGGATTCTCAG
ACTCCACAGGGAATTGTTGCCGTGGTTCAAAAAGAAGAAGTAGGACAAGCTGATTTGAGTCAGGGCAAGTTCTTGTTTTT
GGAAGATGTGCAAGATCCTGGTAATGTAGGAACTATCATTCGAACTGCGGATGCAGCAGGTTTTACAGGAGTGATTGTTT
CAGATAAGTCGGCAGACATCTACAGTCTCAAAACCCTACGTTCCATGCAAGGTAGTCATTTCCATCTGCCCATTTACCGG
ATGACTAGTCAAGCGCTTCTTGACGAAACTAAAAAGGTAGCTATCCCAGTGCTAGCAACAACCCTATCTAAAGATTCTGT
TGATTACAGAGAACTGCCTTCTATAGAAAATTTTGTACTAGTTATGGGAAATGAGGGTCAAGGAATTAGTCCCCTTATGG
CTGAAAGTGCAGACCAGTTGGTCCATATTAGCATGAAGGGGCAGGCCGAGAGTTTGAATGTTGCAGTTGCAGCCGGTATT
TTAATCTTCCATTTAAGCTAA

Upstream 100 bases:

>100_bases
TATAAACACCCCAACGAAAGCCGACTCCCTGCACCCTACCTTGGGCAATCATTCTAACCTTTTGCATACCAAACCCCTTT
GATTGTGTTATAATATTTCT

Downstream 100 bases:

>100_bases
TTTTAACTTTTTTTGTTATAATCAAGGAAAGATGTTCACAGAAAAGGAGAAAAGGATGAATCACACTATTATACATGACC
GTGCAGGTCTCAATCAATTT

Product: possible rRNA/tRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGVTIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQ
TPQGIVAVVQKEEVGQADLSQGKFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESADQLVHISMKGQAESLNVAVAAGI
LIFHLS

Sequences:

>Translated_246_residues
MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGVTIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQ
TPQGIVAVVQKEEVGQADLSQGKFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESADQLVHISMKGQAESLNVAVAAGI
LIFHLS
>Mature_245_residues
TIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGVTIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQT
PQGIVAVVQKEEVGQADLSQGKFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYRM
TSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESADQLVHISMKGQAESLNVAVAAGIL
IFHLS

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=180, Percent_Identity=35, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790083, Length=148, Percent_Identity=31.7567567567568, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26918; Mature: 26787

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGVTIEKIFALENYRNQLA
CEEEECCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQGKFLFLEDVQDPGNVGTII
HCCCEEEECHHHEEEECCCCCCCCEEEEEEHHHCCCCCCCCCCEEEEECCCCCCCCEEEE
RTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYRMTSQALLDETKKVAIPVLAT
EECCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHEEHHHHEE
TLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESADQLVHISMKGQAESLNVAVAAGI
HHCCCCCCHHHCCCCCCEEEEECCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCEEEEEE
LIFHLS
EEEEEC
>Mature Secondary Structure 
TIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGVTIEKIFALENYRNQLA
EEEECCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQGKFLFLEDVQDPGNVGTII
HCCCEEEECHHHEEEECCCCCCCCEEEEEEHHHCCCCCCCCCCEEEEECCCCCCCCEEEE
RTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYRMTSQALLDETKKVAIPVLAT
EECCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHEEHHHHEE
TLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESADQLVHISMKGQAESLNVAVAAGI
HHCCCCCCHHHCCCCCCEEEEECCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCEEEEEE
LIFHLS
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]