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The map label for this gene is lytA

Identifier: 225859701

GI number: 225859701

Start: 1853293

End: 1854249

Strand: Reverse

Name: lytA

Synonym: SP70585_2016

Alternate gene names: 225859701

Gene position: 1854249-1853293 (Counterclockwise)

Preceding gene: 225859702

Following gene: 225859700

Centisome position: 84.88

GC content: 46.08

Gene sequence:

>957_bases
ATGGAAATTAATGTGAGTAAATTAAGAACAGATTTGCCTCAAGTCGGCGTGCAACCATATAGGCAAGTACACGCACACTC
AACTGGGAATCCGCATTCAACCGTACAGAATGAAGCGGATTATCACTGGCGGAAAGACCCAGAATTAGGTTTTTTCTCGC
ACATTGTTGGGAACGGTTGCATCATGCAGGTAGGACCTGTTGATAATGGTGCCTGGGACGTTGGGGGCGGTTGGAATGCT
GAGACCTATGCAGCGGTTGAACTGATTGAAAGCCATTCAACTAAAGAAGAGTTCATGACGGACTACCGCCTTTATATCGA
ACTCTTACGCAATCTAGCAGATGAAGCAGGTTTGCCGAAAACGCTTGATACAGGGAGTTTAGCTGGAATTAAAACGCACG
AGTATTGCACGAATAACCAACCAAACAACCACTCAGACCACGTTGACCCTTATCCATATCTTGCTAAATGGGGCATTAGC
CGTGAGCAGTTTAAGCATGATATTGAGAACGGCTTGACGATTGAAACAGGCTGGCAGAAGAATGACACTGGCTACTGGTA
CGTACATTCAGACGGCTCTTATCCAAAAGACAAGTTTGAGAAAATCAATGGCACTTGGTACTACTTTGACAGTTCAGGCT
ATATGCTTGCAGACCGCTGGAGGAAGCACACAGACGGCAACTGGTACTGGTTCGACAACTCAGGCGAAATGGCTACAGGC
TGGAAGAAAATTGCTGATAAGTGGTACTATTTCAACGAAGAAGGTGCCATGAAGACAGGCTGGGTCAAGTACAAGGACAC
TTGGTACTACTTAGACGCTAAAGAAGGCGCCATGGTATCAAATGCCTTTATCCAGTCAGCGGACGGAACAGGCTGGTACT
ACCTCAAACCAGACGGAACACTGGCAGACAAGCCAGAATTCACAGTAGAGCCAGATGGCTTGATTACAGTAAAATAA

Upstream 100 bases:

>100_bases
TAATCGTGACTAAGATTGTCTTCTTTGTAAGGTAGAAATAAAGGAGTTTCTGGTTCTGGATTGTAAAAAATGAGTTGTTT
TAATTGATAAGGAGTAGAAT

Downstream 100 bases:

>100_bases
TAATGGAATGTCTTTCAAATCAGAACAGCGCATATTATTAGGTCTTGAAAAAGCTTAATAGTATGCGTTTTTTTGTGGAG
ATATTTCCTTCAATGTTTGC

Product: autolysin (N-acetylmuramoyl-L-alanine amidase) (Mureinhydrolase) (Mucopeptide aminohydrolase) (Cell wall hydrolase)

Products: NA

Alternate protein names: Cell wall hydrolase; Mucopeptide aminohydrolase; Murein hydrolase; N-acetylmuramoyl-L-alanine amidase

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWRKDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNA
ETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLADRWRKHTDGNWYWFDNSGEMATG
WKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK

Sequences:

>Translated_318_residues
MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWRKDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNA
ETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLADRWRKHTDGNWYWFDNSGEMATG
WKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK
>Mature_318_residues
MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWRKDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNA
ETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLADRWRKHTDGNWYWFDNSGEMATG
WKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 6 cell wall-binding repeats

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALYS_STRPN (P06653)

Other databases:

- EMBL:   M13812
- EMBL:   Z34303
- EMBL:   AE005672
- PIR:   A25634
- PIR:   D95226
- PIR:   H98090
- RefSeq:   NP_346365.1
- PDB:   1GVM
- PDB:   1H8G
- PDB:   1HCX
- PDB:   2BML
- PDBsum:   1GVM
- PDBsum:   1H8G
- PDBsum:   1HCX
- PDBsum:   2BML
- ProteinModelPortal:   P06653
- SMR:   P06653
- EnsemblBacteria:   EBSTRT00000026683
- GeneID:   931994
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_1937
- TIGR:   SP_1937
- GeneTree:   EBGT00050000026602
- HOGENOM:   HBG702446
- ProtClustDB:   CLSK560258
- BioCyc:   SPNE170187-1:SP_1937-MONOMER
- BRENDA:   3.5.1.28
- InterPro:   IPR002502
- InterPro:   IPR018337
- SMART:   SM00644

Pfam domain/function: PF01510 Amidase_2; PF01473 CW_binding_1; SSF55846 Amidase_2

EC number: =3.5.1.28

Molecular weight: Translated: 36545; Mature: 36545

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS51170 CW

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWRKDPELGFFSHIVGNGC
CCCCHHHHHHCCHHCCCCHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHCCCE
IMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPK
EEEECCCCCCCEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGISREQFKHDIENGLTIETGWQK
CCCCCCCCCCEEHHHHCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCEEEECCCC
NDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLADRWRKHTDGNWYWFDNSGEMATG
CCCEEEEEECCCCCCHHHHHHCCCEEEEECCCCCEEHHHHHCCCCCCEEEECCCCCCHHH
WKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGT
HHHHHHHHEEECCCCCCEECEEEEECEEEEEECCCCCEEEHHHEECCCCCEEEEECCCCC
LADKPEFTVEPDGLITVK
CCCCCCEEECCCCEEEEC
>Mature Secondary Structure
MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWRKDPELGFFSHIVGNGC
CCCCHHHHHHCCHHCCCCHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHCCCE
IMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPK
EEEECCCCCCCEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGISREQFKHDIENGLTIETGWQK
CCCCCCCCCCEEHHHHCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCEEEECCCC
NDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLADRWRKHTDGNWYWFDNSGEMATG
CCCEEEEEECCCCCCHHHHHHCCCEEEEECCCCCEEHHHHHCCCCCCEEEECCCCCCHHH
WKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGT
HHHHHHHHEEECCCCCCEECEEEEECEEEEEECCCCCEEEHHHEECCCCCEEEEECCCCC
LADKPEFTVEPDGLITVK
CCCCCCEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2875013; 11463916