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The map label for this gene is rumA2

Identifier: 225859667

GI number: 225859667

Start: 1829703

End: 1831058

Strand: Reverse

Name: rumA2

Synonym: SP70585_1982

Alternate gene names: 225859667

Gene position: 1831058-1829703 (Counterclockwise)

Preceding gene: 225859670

Following gene: 225859663

Centisome position: 83.81

GC content: 37.68

Gene sequence:

>1356_bases
ATGAATCTGAAAGTGAAACAAAAAATACCATTAAAAATCAAACGCATGGGAATTAATGGTGAGGGAATCGGCTTTTACCA
AAAAACATTAGTCTTTGTACCTGGAGCTCTCAAAGGCGAAGATATCTATTGTCAGATTACTTCTATTAGACGCAACTTTG
TTGAAGCAAAATTACTGAAGGTCAACAAGAAGTCTAAATTTCGAATTGTGCCATCTTGTACTATTTATAATGAATGCGGA
GGCTGCCAAATCATGCACCTGCATTATGATAAGCAGCTGGAGTTCAAGACGGACTTACTTCATCAAGCGCTGAAAAAATT
TGCTCCTGCAGGATATGAAAATTATGAAATTCGTCCAACTATTGGAATGCAGGAACCAAAATATTACAGAGCTAAGTTAC
AATTTCAGACTCGAAAATTTAAAAATCAGGTCAAGGCGGGCTTATATGCACAAAACTCTCACTATTTAGTAGAGTTGAAA
GACTGCCTGGTACAAGATAAGGAAACCCAAGTGATTGCTAATCGCTTAGCAGAATTACTTACTTATCACCAGATTCCAAT
CACGGATGAGAGAAAAGTTCTAGGTGTCCGTACTATTATGGTCCGACGCGCGAGAAAGACCGGACAGGTTCAGATTATTA
TTGTTACAAACCGCCAGCTTAATTTAACCCAACTGGTAAAAGAGTTGGTTAAAGGTTTTCCAGAAGTTGTGACAGTAGCT
GTTAATACAAATACAGCTAAAACCAGTGAGATATATGGTGAAAAGACAGAGATTATCTGGGGACAAGAGAGTATTCAAGA
AGGTGTACTCGATTATGAATTTTCACTATCCCCTCGAGCTTTCTATCAACTAAATCCTGAACAAACAGAAGTCCTTTATA
GCGAGGTAGTAAAAGCGCTGGATGTTAGTAAAGAAGACCATTTGATTGACGCTTATTGTGGAGTTGGAACAATTGGTTTT
GCCTTTGCAAAGAAAGTAAAAACACTCAGAGGTATGGATATTATTCCAGAAGCTATTGAAGATGCCAAGCGAAATGCTAA
AAGAATGGGATTTGACAATACTCATTATGAAGCTGGAACGGCAGAAGAGATTATTCCTCGTTGGTACAAGGAAGGCTACC
GAGCAGATGCTTTGATTGTTGACCCACCACGTACAGGTCTGGATGATAAGTTATTAGATACTATTCTTACTTATGTACCA
GAAAAAATGGTTTATATTTCTTGTAATGTTTCGACCTTGGCTCGTGATTTGGTACGCTTAGTAGAAGTCTATGATCTTCA
TTATATCCAGTCGGTCGATATGTTCCCACATACAGCTCGAACTGAAGCTGTTGTAAAATTAATAAAAAAAGTTTAA

Upstream 100 bases:

>100_bases
GTCGCCATTATCAAGCTCCATCAGATAGAGTCTTTTTTTCTTTTCAAGTTTTGTGATTTTCATAGCTCTATTATAACTCA
AAATGTGATAAGATAGGGGT

Downstream 100 bases:

>100_bases
AAAAGGTGTTGACAAAGTTTGAAAAGACTGTATAATAGTAAGAGTTGAAAATAACAACTCAGGTCCGTTGGTCAAGGGGT
TAAGACACCGCCTTTTCACG

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECG
GCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKGFPEVVTVA
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLDYEFSLSPRAFYQLNPEQTEVLYSEVVKALDVSKEDHLIDAYCGVGTIGF
AFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVP
EKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV

Sequences:

>Translated_451_residues
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECG
GCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKGFPEVVTVA
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLDYEFSLSPRAFYQLNPEQTEVLYSEVVKALDVSKEDHLIDAYCGVGTIGF
AFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVP
EKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV
>Mature_451_residues
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECG
GCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKGFPEVVTVA
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLDYEFSLSPRAFYQLNPEQTEVLYSEVVKALDVSKEDHLIDAYCGVGTIGF
AFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVP
EKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=210, Percent_Identity=31.9047619047619, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI51173878, Length=210, Percent_Identity=31.9047619047619, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI269784680, Length=204, Percent_Identity=29.4117647058824, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI269784676, Length=204, Percent_Identity=29.4117647058824, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI20149688, Length=204, Percent_Identity=29.4117647058824, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI269784678, Length=277, Percent_Identity=27.4368231046931, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1789148, Length=451, Percent_Identity=25.7206208425721, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787083, Length=387, Percent_Identity=25.5813953488372, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790403, Length=166, Percent_Identity=28.9156626506024, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71997438, Length=163, Percent_Identity=28.2208588957055, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322909, Length=432, Percent_Identity=23.1481481481481, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24666579, Length=422, Percent_Identity=26.303317535545, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51686; Mature: 51686

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLK
CCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHE
VNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPT
ECCCCCEEEECCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
IGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELKDCLVQDKETQVIANRLAELL
CCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEHHHHHCCCHHHHHHHHHHHHH
TYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKGFPEVVTVA
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEE
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLDYEFSLSPRAFYQLNPEQTEVLYSEVVKAL
EECCCCCHHHHCCCCEEEEECHHHHHCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHH
DVSKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGT
CCCCCCCEEEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
AEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVPEKMVYISCNVSTLARDLVRL
HHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHH
VEVYDLHYIQSVDMFPHTARTEAVVKLIKKV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYCQITSIRRNFVEAKLLK
CCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHE
VNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPT
ECCCCCEEEECCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
IGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELKDCLVQDKETQVIANRLAELL
CCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEHHHHHCCCHHHHHHHHHHHHH
TYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKGFPEVVTVA
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEE
VNTNTAKTSEIYGEKTEIIWGQESIQEGVLDYEFSLSPRAFYQLNPEQTEVLYSEVVKAL
EECCCCCHHHHCCCCEEEEECHHHHHCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHH
DVSKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGT
CCCCCCCEEEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
AEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVPEKMVYISCNVSTLARDLVRL
HHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHH
VEVYDLHYIQSVDMFPHTARTEAVVKLIKKV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11544234 [H]