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The map label for this gene is wcaJ [H]

Identifier: 225859601

GI number: 225859601

Start: 1758623

End: 1759315

Strand: Reverse

Name: wcaJ [H]

Synonym: SP70585_1892

Alternate gene names: 225859601

Gene position: 1759315-1758623 (Counterclockwise)

Preceding gene: 225859602

Following gene: 225859600

Centisome position: 80.53

GC content: 46.75

Gene sequence:

>693_bases
ATGCTGAAATGGGAAGACTTGCCTGTGGAAATGAAATCAAGCGAGGTTGAGTTTTACTACCAGCTTGTCTCTAAAAGGAA
GGGGTCGCTGATTTTCAAGCGTTGCTTGGCCTTGGTTTTGGCCTTGGTCTTACTGGTTCTGACCTCTCCCATCTTTCTCA
TCTTGAGCATTTGGATCAAGTTGGATAGCAAGGGGCCAGTGATTTACAAGCAAGAGCGTGTGACCCAGTACAACCGTCGG
TTCAAGATTTGGAAGTTCCGTACCATGGTGATGGATGCGGATAAAAAAGGAAGTCTGGTGACTTCTGCTAACGATAGCCG
CATTACCAAGGTTGGAAATTTCATCCGACGTGTTCGTTTGGACGAACTGCCTCAGTTGGTCAATGTCCTTAAAGGTGAGA
TGTCCTTTGTCGGTACACGACCTGAAGTGCCACGTTATACAGAGCAGTACAGCCCTGAAATGCTGGCAACCTTGCTCTTG
CCAGCAGGGATTACCTCTCCAGCCAGCATCAACTACAAGGATGAGGACACCATCATCAGTCAAATGACGGAGAAAGGTCT
GTCAGCTGATCAGGCCTATGTGGAGCATGTTCTTCCTGAAAAGATGCGCTATAACCTCGCCTATCTCCGAGAGTTTAGTT
TCCTTGGGGACATCAAAATCATGTTTCAAACCGTGTTTGAGGTACTAAAATAA

Upstream 100 bases:

>100_bases
GAAATCCTTCAAATTACAGATTTCTTTCACCGCATTTTCCATTTTGTGGTATAATGAAAAATGTTGACAAATAGTATAAT
AAAAACAAAGGAGAAACCGC

Downstream 100 bases:

>100_bases
AGTAGTCATAAGAAAATGAGTACAGATAAAAGGAGAAAAGCAATGCCAAATTACAATATTCCATTTTCACCGCCTGATAT
CACAGAAGCAGAAATTGCTG

Product: sugar transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MLKWEDLPVEMKSSEVEFYYQLVSKRKGSLIFKRCLALVLALVLLVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRR
FKIWKFRTMVMDADKKGSLVTSANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMLATLLL
PAGITSPASINYKDEDTIISQMTEKGLSADQAYVEHVLPEKMRYNLAYLREFSFLGDIKIMFQTVFEVLK

Sequences:

>Translated_230_residues
MLKWEDLPVEMKSSEVEFYYQLVSKRKGSLIFKRCLALVLALVLLVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRR
FKIWKFRTMVMDADKKGSLVTSANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMLATLLL
PAGITSPASINYKDEDTIISQMTEKGLSADQAYVEHVLPEKMRYNLAYLREFSFLGDIKIMFQTVFEVLK
>Mature_230_residues
MLKWEDLPVEMKSSEVEFYYQLVSKRKGSLIFKRCLALVLALVLLVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRR
FKIWKFRTMVMDADKKGSLVTSANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMLATLLL
PAGITSPASINYKDEDTIISQMTEKGLSADQAYVEHVLPEKMRYNLAYLREFSFLGDIKIMFQTVFEVLK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=204, Percent_Identity=40.6862745098039, Blast_Score=138, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 26648; Mature: 26648

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKWEDLPVEMKSSEVEFYYQLVSKRKGSLIFKRCLALVLALVLLVLTSPIFLILSIWIK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSKGPVIYKQERVTQYNRRFKIWKFRTMVMDADKKGSLVTSANDSRITKVGNFIRRVRL
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHH
DELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMLATLLLPAGITSPASINYKDEDTIIS
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
QMTEKGLSADQAYVEHVLPEKMRYNLAYLREFSFLGDIKIMFQTVFEVLK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLKWEDLPVEMKSSEVEFYYQLVSKRKGSLIFKRCLALVLALVLLVLTSPIFLILSIWIK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSKGPVIYKQERVTQYNRRFKIWKFRTMVMDADKKGSLVTSANDSRITKVGNFIRRVRL
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHH
DELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMLATLLLPAGITSPASINYKDEDTIIS
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
QMTEKGLSADQAYVEHVLPEKMRYNLAYLREFSFLGDIKIMFQTVFEVLK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]