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The map label for this gene is ywbA [H]

Identifier: 225859571

GI number: 225859571

Start: 1729456

End: 1730811

Strand: Reverse

Name: ywbA [H]

Synonym: SP70585_1862

Alternate gene names: 225859571

Gene position: 1730811-1729456 (Counterclockwise)

Preceding gene: 225859572

Following gene: 225859570

Centisome position: 79.22

GC content: 36.58

Gene sequence:

>1356_bases
ATGAATACAATGTTGGATAAAATGCAAGAAAAACTTTCTCCAATTGCAATGAAAGTTGGGAATCAGAAGTTTTTAGTTGC
TTTACGTGATTCATTTGTGGGGACTATGCCTGTTATTATGGCAGGTTCCATTGCTTTGTTATTAAATGCTTTTCTAGTGG
ATTTACCACAACAATTTCACCTAGAAAGTATTACGAAAACCTTTCAGTGGCTTGTTGACATCAATAATTTGGTATTCAAG
GGAAGTATACCAATTGTTTCCTTGTTGTTCATCTATTGCTTGGGAGTGAATATCGCTAAGATTTACAAGGTTGATACAGT
TTCTGCTGGTTTGGTATCACTTGCTTCGTTTGTCATTTCGATTGGAAGTACAGTTACAAAGAGTTTCCCTTTGGCAAATG
TAGGAGATGTGAAATTAGATCAGATCTTACAAGGAATTGATAATCTAGCATTTGATGGTAAAAATCTGATGGTAACTATT
GGGAATGTGATTCCAGGAAACCATATTAATGCCAGAGGCTACTTTACAGCCATGATGATTGGATTTTTAGCCTCTATTAT
TTTCTGTAAAGTAATGAAAAAGAACTGGGTAATCAAGTTACCAGATTCAGTTCCCCCTGCGATTGCTAAGCCGTTCACTT
CTATCATTCCAGGATTTATGGCGATGTATATAGTAGCCATCTTGACTTATGTTTTCCATTTACTTAGCAATGACTTATTG
ATTGATTGGGTTTACAAGGTGTTACAAACGCCATTACTAGGTTTGTCTCAAAGTTTCTTTGCAGTTATTCTGATGATCTT
TTTGAATAAGTTATTCTGGTTTTTTGGTCTCCATGGAGGAAATGTATTGGCTCCCATCATGGAAGGGCTATTTGGAGTTG
CTATGTTAGCGAATCTGGATGCTTTCCAAAAAGGTGAACCGATTCCTTATATATGGACAAGTGGGTCTTTTGGGGCGTTT
GTCTGGTTTGGAGGATTAGGATTAGTACTTGCTATTTTAATTTTTTCAAGAAACAGTCATTATCGAAAAGTTGCTAAACT
TGGTCTAGCACCAGTTCTGTTTAATATTGGTGAGCCGGTCAATTATGGTTTACCAGTTGTCTTGAATCCTTTGCTATTTA
TACCATTTGTACTTAGCCCGGTTTTCATGGCAACGGTAGCTTACTGGGCAACAAGCTGGGGTCTTGTTTCACCTGTTACA
CAAAATGTTACTTGGGTAATGCCTCCAATTTTATATGGTTTCTTCTCTACAGCATTTGATTGGCGTGCCATCATCTTATC
AGTTGTTTGTTTGATTATTAGTGTCTTGACTTATTTCCCATTTGTGAAAATGGCAGATAAAACTGAATTGAGTTAA

Upstream 100 bases:

>100_bases
ACACGCGCAAGATCATTTAATGACGAGTCTGACTTTTGTTGACTTGGCGAAAGAAGTGGTAGAAGTATACGAACGATTTG
AAAAAAATTAGGAGTTTGGT

Downstream 100 bases:

>100_bases
TGGTCTTACTTGCGTTTTTTGTGATAAAATAGAAATAGATTACGTGTAAGATGAGTTAGGTGTAGGATTTCTTATCTCCA
CTAAAATTGTTATTAGACAA

Product: phosphotransferase system

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Putative PTS system EIIC component [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMAGSIALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFK
GSIPIVSLLFIYCLGVNIAKIYKVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPGFMAMYIVAILTYVFHLLSNDLL
IDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFGLHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAF
VWFGGLGLVLAILIFSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATSWGLVSPVT
QNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTELS

Sequences:

>Translated_451_residues
MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMAGSIALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFK
GSIPIVSLLFIYCLGVNIAKIYKVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPGFMAMYIVAILTYVFHLLSNDLL
IDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFGLHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAF
VWFGGLGLVLAILIFSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATSWGLVSPVT
QNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTELS
>Mature_451_residues
MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMAGSIALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFK
GSIPIVSLLFIYCLGVNIAKIYKVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPGFMAMYIVAILTYVFHLLSNDLL
IDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFGLHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAF
VWFGGLGLVLAILIFSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATSWGLVSPVT
QNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTELS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=471, Percent_Identity=29.0870488322718, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 49742; Mature: 49742

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMAGSIALLLNAFLVDLPQQFH
CCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIYKVDTVSAGLVSLASFVIS
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCHHHHEEEHHHHHHHHHHHHHHHH
IGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTIGNVIPGNHINARGYFTAMMI
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHH
GFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPGFMAMYIVAILTYVFHLLSNDLL
HHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFGLHGGNVLAPIMEGLFGVAMLANLD
HHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCH
AFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILIFSRNSHYRKVAKLGLAPVLFNIGEPV
HHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
NYGLPVVLNPLLFIPFVLSPVFMATVAYWATSWGLVSPVTQNVTWVMPPILYGFFSTAFD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHH
WRAIILSVVCLIISVLTYFPFVKMADKTELS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMAGSIALLLNAFLVDLPQQFH
CCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIYKVDTVSAGLVSLASFVIS
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCHHHHEEEHHHHHHHHHHHHHHHH
IGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTIGNVIPGNHINARGYFTAMMI
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHH
GFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPGFMAMYIVAILTYVFHLLSNDLL
HHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFGLHGGNVLAPIMEGLFGVAMLANLD
HHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCH
AFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILIFSRNSHYRKVAKLGLAPVLFNIGEPV
HHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
NYGLPVVLNPLLFIPFVLSPVFMATVAYWATSWGLVSPVTQNVTWVMPPILYGFFSTAFD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHH
WRAIILSVVCLIISVLTYFPFVKMADKTELS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]