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The map label for this gene is pksG [H]

Identifier: 225859479

GI number: 225859479

Start: 1640438

End: 1641634

Strand: Reverse

Name: pksG [H]

Synonym: SP70585_1766

Alternate gene names: 225859479

Gene position: 1641634-1640438 (Counterclockwise)

Preceding gene: 225859480

Following gene: 225859478

Centisome position: 75.14

GC content: 42.11

Gene sequence:

>1197_bases
ATGAATGATAAAACAGAGGTAAATATGACAATCGGTATTGATAAGATTGGTTTTGCGACCAGTCAATATGTCTTGAAATT
ACAAGACTTAGCAGAAGCGAGGGGAATTGACCCTGAAAAATTAAGTAAAGGACTCTTACTCAAGGAATTGAGTATTGCGC
CCCTAACTGAGGACATTGTGACCTTGGCGGCCAGTGCTAGTGACTCTATTTTAACTGAGCAAGAAAGACAAGAAGTTGAC
ATGGTCATTGTGGCTACCGAGTCAGGAATTGACCAGAGTAAGGCTGCGGCCGTCTTTGTGCATGGCTTGCTGGGCATCCA
GCCCTTTGCTCGTAGTTTCGAGATTAAAGAAGCCTGCTACGGAGCGACTGCTGCCCTTCATTATGCCAAATTGCATGTGG
AAAATTCTCCGGAGTCCAAGGTCTTGGTCATTGCCAGTGATATTGCCAAATACGGTATTGAAACTCCAGGAGAACCAACT
CAAGGTGCTGGAAGTGTAGCTATGTTGATTACACAAAATCCACGCATGATGGCCTTTAATAATGACAATGTAGCTCAGAC
CCGTGACATCATGGATTTCTGGCGACCAAATTACTCGACAACTCCTTATGTAAATGGTGTTTATTCTACCCAACAATACT
TGGATAGTTTGAAAACGACTTGGCTTGAATATCAAAAACGCTACCAGCTTACTCTGGATGATTTTGCGGCTGTTTGTTTC
CACTTGCCTTATCCTAAATTAGCGCTAAAAGGCTTGAAAAAAATCATGGATAAGAGCCTGCCTCAAGAGAAAAAAGACCT
CTTACAAAAGCATTTTGACCAGTCTATTCTCTACAGTCAAAAGGTGGGGAATATCTACACAGGTTCACTTTTCCTTGGAC
TTTTGTCTCTCTTGGAAAATACAGATAGCTTGAAAGCTGGGGATAAAATCGCCCTTTATAGTTACGGAAGTGGAGCTGTG
GCTGAGTTCTTCAGTGGTGAATTGGTTGAAGGATATGAAGCTTATTTGGATAAAGACCGCTTGAACAAGCTCAACCAACG
AACTGCCCTATCCGTTGCAGACTATGAAAAGGTCTTTTTTGAGGAAGTAAACTTGGATGAAACAAACTCTGCCCAGTTTG
CTGGCTATGAAAATCAAGATTTTGCCTTGGTTGAAATTCTCGACCACCAACGCCGTTATAGCAAGGTTGAAAAATAA

Upstream 100 bases:

>100_bases
TTTAAAATGATGACCAATAAGAGGCTTGATTTGGTGAATCAAGTCTTTTTTCTCTCTTTTACTGCCATTTGTGATAAAAT
AGTATAGAACGATTTTTTAC

Downstream 100 bases:

>100_bases
TGAAGATAAGTTGGAATGGATTTTCTAAAAAATCATACCAAGAGCGCCTCGAGCTGTTAAAAGCTCAGGCGCTCCTTAGT
CCTGAGAGACAAGCTAGTCT

Product: hydroxymethylglutaryl-CoA synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNDKTEVNMTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSIAPLTEDIVTLAASASDSILTEQERQEVD
MVIVATESGIDQSKAAAVFVHGLLGIQPFARSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPT
QGAGSVAMLITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQKRYQLTLDDFAAVCF
HLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGNIYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAV
AEFFSGELVEGYEAYLDKDRLNKLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK

Sequences:

>Translated_398_residues
MNDKTEVNMTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSIAPLTEDIVTLAASASDSILTEQERQEVD
MVIVATESGIDQSKAAAVFVHGLLGIQPFARSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPT
QGAGSVAMLITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQKRYQLTLDDFAAVCF
HLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGNIYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAV
AEFFSGELVEGYEAYLDKDRLNKLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK
>Mature_398_residues
MNDKTEVNMTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSIAPLTEDIVTLAASASDSILTEQERQEVD
MVIVATESGIDQSKAAAVFVHGLLGIQPFARSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPT
QGAGSVAMLITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQKRYQLTLDDFAAVCF
HLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGNIYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAV
AEFFSGELVEGYEAYLDKDRLNKLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI5031751, Length=449, Percent_Identity=27.1714922048998, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI148298764, Length=416, Percent_Identity=27.1634615384615, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI148298677, Length=416, Percent_Identity=27.1634615384615, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560126, Length=371, Percent_Identity=25.3369272237197, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323509, Length=400, Percent_Identity=24.5, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24654139, Length=374, Percent_Identity=27.5401069518717, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24654137, Length=374, Percent_Identity=27.5401069518717, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24654133, Length=374, Percent_Identity=27.5401069518717, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24654135, Length=374, Percent_Identity=27.5401069518717, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17933694, Length=374, Percent_Identity=27.5401069518717, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 44436; Mature: 44436

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKTEVNMTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSIAPLTEDIV
CCCCEEEEEEEECHHHCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHCCCCCCHHHHH
TLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFARSFEIKEACY
HHHHCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAMLITQNPRMMAFN
HHHHHHHEEEEEECCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEEC
NDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQKRYQLTLDDFAAVCF
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGNIYTGSLFLGLLSLLEN
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCC
TDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLNKLNQRTALSVADYEKVFF
CCCCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
EEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDKTEVNMTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSIAPLTEDIV
CCCCEEEEEEEECHHHCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHCCCCCCHHHHH
TLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFARSFEIKEACY
HHHHCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAMLITQNPRMMAFN
HHHHHHHEEEEEECCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEEC
NDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQKRYQLTLDDFAAVCF
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGNIYTGSLFLGLLSLLEN
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCC
TDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLNKLNQRTALSVADYEKVFF
CCCCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
EEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]