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The map label for this gene is murF [H]

Identifier: 225859425

GI number: 225859425

Start: 1580045

End: 1581418

Strand: Reverse

Name: murF [H]

Synonym: SP70585_1710

Alternate gene names: 225859425

Gene position: 1581418-1580045 (Counterclockwise)

Preceding gene: 225859426

Following gene: 225859424

Centisome position: 72.39

GC content: 42.21

Gene sequence:

>1374_bases
ATGAAATTAACAATCTATGAAGTTGCCCAAGCTGTTGGAGCTAAAAATGATGTTAGTCTTTTTGCGGACGCCCAGTTAGA
AAAGGCTGAGTTTGACAGTCGATTGATTGGGACTGGAGATTTATTTGTGCCACTTAAAGGTGCGCGTGATGGCCATGACT
TTATTGAAACAGCCTTTGAAAATGGTGCAGCAGTAACCTTGTCTGAGAAAGAGGTCTCAAATCATCCTTACATTCTAGTA
GATGATGTTTTGACAGCCTTTCAATCCTTAGCATCCTACTATCTTGAAAAAACGACTGTTGATGTCTTTGCTGTTACAGG
TTCAAATGGCAAGACAACGACTAAGGATATGTTGGCGCATTTACTGTCAACAAGATACAAGACCTACAAAACACAAGGCA
ATTACAATAATGAGATTGGCCTTCCTTACACAGTTCTTCATATGCCTGAAGGAACAGAAAAGTTGGTTTTGGAGATGGGA
CAGGATCACTTGGGAGATATTCATCTCTTGTCTGAATTGGCTCGTCCAAAAACAGCCATCGTGACCTTGGTTGGAGAAGC
TCATTTGGCCTTTTTCAAAGATCGTTCTGAGATTGCTAAAGGAAAAATGCAAATTGCAGACGGAATGGCTTCAGGTTCCT
TGCTTTTAACGCCGGCTGACCCTATCGTAGAGGACTATTTGCCAACTGATAAAAAGGTGGTCCGTTTTGGGCAAGGGGCA
GAGTTGGAAATCACAGACTTGGTTGAGCGTAAGGACAGTCTGACCTTTAAGGCTAATTTCTTGGAACAAGCCCTTGATTT
GCCAGTGACTGGTAAGTACAATGCTACCAATGCTATGATTGCGTCCTATGTTGCCCTACAAGAAGGAGTTTCAGAGGAGC
AAATTCATCAGGCCTTCCAAGGTCTTGAATTGACGCGTAATCGTACTGAGTGGAAGAAAGCAGCCAATGGAGCAGATATC
CTGTCAGACGTATATAATGCCAATCCAACTGCTATGAAGCTGATTTTAGAGACTTTCTCTGCCATTCCAGCCAATGAAGG
TGGCAAGAAAATTGCAGTGTTGGCGGATATGAAGGAGCTTGGTGACCAGTCTATTCAACTCCATAACCAGATAATTTTGA
GCCTTTCTCCAGATGTGCTTGATACCGTTATTTTCTATGGAGAAGACATTGCTGAATTAGCCCAACTTGCTAGTCAAATG
TTCCCAATCGGACACGTTTATTACTTCAAGAAAACAGAAGACCAAGACCAATTTGAAGACCTAGTTAAGCAGGTCAAAGA
AAGCCTTGGGGCTCATGACCAAATCCTGCTCAAAGGCTCTAACTCTATGAATCTAGCCAAGTTGGTAGAAAGTTTAGAAA
ATGAAGACAAGTGA

Upstream 100 bases:

>100_bases
AAGCGCATTTGATATAAAATGAAAGAGAGGGTAGAAGCCAGAACCATCACTGCAAGGTGACTAGAGTTCTCGGACTTCGC
CCCTTTTTAAAGGAGTAGAA

Downstream 100 bases:

>100_bases
TTTTGTCAAGTATTTGCAAAGAATGATTGCCATTACAGATACTGGCTTAACCTTTACAAAAGATCCGTTTGACCGTGAGC
GCTACGAAGACTTGCGAGAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKLTIYEVAQAVGAKNDVSLFADAQLEKAEFDSRLIGTGDLFVPLKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILV
DDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLTPADPIVEDYLPTDKKVVRFGQGA
ELEITDLVERKDSLTFKANFLEQALDLPVTGKYNATNAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADI
LSDVYNANPTAMKLILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQIILSLSPDVLDTVIFYGEDIAELAQLASQM
FPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVESLENEDK

Sequences:

>Translated_457_residues
MKLTIYEVAQAVGAKNDVSLFADAQLEKAEFDSRLIGTGDLFVPLKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILV
DDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLTPADPIVEDYLPTDKKVVRFGQGA
ELEITDLVERKDSLTFKANFLEQALDLPVTGKYNATNAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADI
LSDVYNANPTAMKLILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQIILSLSPDVLDTVIFYGEDIAELAQLASQM
FPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVESLENEDK
>Mature_457_residues
MKLTIYEVAQAVGAKNDVSLFADAQLEKAEFDSRLIGTGDLFVPLKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILV
DDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLTPADPIVEDYLPTDKKVVRFGQGA
ELEITDLVERKDSLTFKANFLEQALDLPVTGKYNATNAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADI
LSDVYNANPTAMKLILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQIILSLSPDVLDTVIFYGEDIAELAQLASQM
FPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVESLENEDK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=444, Percent_Identity=29.2792792792793, Blast_Score=148, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50304; Mature: 50304

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTIYEVAQAVGAKNDVSLFADAQLEKAEFDSRLIGTGDLFVPLKGARDGHDFIETAFE
CEEEHHHHHHHHCCCCCCEEEECCHHHHHHCCCEEECCCCEEEEECCCCCCHHHHHHHHC
NGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKDMLAH
CCCEEEEEHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHH
LLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMGQDHLGDIHLLSELARPKTAI
HHHHHHHHEECCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEE
VTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLTPADPIVEDYLPTDKKVVRFGQGA
EEEECCHHHHHHHHHHHHHHCHHHHHCCCCCCCEEECCCCHHHHHHCCCCHHHHCCCCCC
ELEITDLVERKDSLTFKANFLEQALDLPVTGKYNATNAMIASYVALQEGVSEEQIHQAFQ
CEEHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
GLELTRNRTEWKKAANGADILSDVYNANPTAMKLILETFSAIPANEGGKKIAVLADMKEL
HHHHHCCHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHH
GDQSIQLHNQIILSLSPDVLDTVIFYGEDIAELAQLASQMFPIGHVYYFKKTEDQDQFED
CCHHHHHHHEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LVKQVKESLGAHDQILLKGSNSMNLAKLVESLENEDK
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKLTIYEVAQAVGAKNDVSLFADAQLEKAEFDSRLIGTGDLFVPLKGARDGHDFIETAFE
CEEEHHHHHHHHCCCCCCEEEECCHHHHHHCCCEEECCCCEEEEECCCCCCHHHHHHHHC
NGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKDMLAH
CCCEEEEEHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHH
LLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMGQDHLGDIHLLSELARPKTAI
HHHHHHHHEECCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEE
VTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLTPADPIVEDYLPTDKKVVRFGQGA
EEEECCHHHHHHHHHHHHHHCHHHHHCCCCCCCEEECCCCHHHHHHCCCCHHHHCCCCCC
ELEITDLVERKDSLTFKANFLEQALDLPVTGKYNATNAMIASYVALQEGVSEEQIHQAFQ
CEEHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
GLELTRNRTEWKKAANGADILSDVYNANPTAMKLILETFSAIPANEGGKKIAVLADMKEL
HHHHHCCHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHH
GDQSIQLHNQIILSLSPDVLDTVIFYGEDIAELAQLASQMFPIGHVYYFKKTEDQDQFED
CCHHHHHHHEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LVKQVKESLGAHDQILLKGSNSMNLAKLVESLENEDK
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]