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The map label for this gene is folA

Identifier: 225859328

GI number: 225859328

Start: 1492036

End: 1492542

Strand: Reverse

Name: folA

Synonym: SP70585_1612

Alternate gene names: 225859328

Gene position: 1492542-1492036 (Counterclockwise)

Preceding gene: 225859329

Following gene: 225859327

Centisome position: 68.32

GC content: 43.0

Gene sequence:

>507_bases
ATGACTAAGAAAATCGTAGCTATTTGGGCCCAGGATGAAGAGGGTGTGATTGGTAAGGAAAATCGTCTGCCTTGGCATTT
GCCAGCAGAATTGCAGCACTTTAAAGAAACAACTCTGAATCATGCTATCTTGATGGGGCGTGTGACCTTTGATGGGATGG
GGCGTCGCTTGCTTCCAAAACGGGAGACCTTGATTTTGACTCGTAACCCAGAAGAAAAGATAGATGGGGTTGCTACTTTT
CACGATATCCAGTCTGTCTTGGACTGGTATCAGGGTCAAGACAAGAATCTCTACATTCTCGGTGGGAAGCAGATTTTTCA
GGCTTTTGAACCCTATTTGGATGAAGTGATTGTGACTCACATTCATGCTCGGGTGGAGGGAGATACCTATTTTCCTGAGG
AGTTTGACTTATCTCTTTTTGAGACAGTTTCAAGCAAATTTTACGCCAAAGATGAGAAAAATCCTTATGATTTTACCATC
CAATACCGCAAGAGAAAGGAAGTCTAA

Upstream 100 bases:

>100_bases
GAAATTTCTTACAGATTTTTTCTATTTTGTAAGCTATTCCAAACCAGTCTTTTTTTGAGTTTTTTGATAAAATAGTACTA
TCAGTGAAAAGGATGGAAGC

Downstream 100 bases:

>100_bases
TGGAACGGAGTTTATTTGGTCTATTCACGGCTTTTCTGTGTTTCATCTGCTTTCTGGCAGGAGCACAGGCTTTTCGTAAA
AAGCGTTACGGGCTTTCTAT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATF
HDIQSVLDWYQGQDKNLYILGGKQIFQAFEPYLDEVIVTHIHARVEGDTYFPEEFDLSLFETVSSKFYAKDEKNPYDFTI
QYRKRKEV

Sequences:

>Translated_168_residues
MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATF
HDIQSVLDWYQGQDKNLYILGGKQIFQAFEPYLDEVIVTHIHARVEGDTYFPEEFDLSLFETVSSKFYAKDEKNPYDFTI
QYRKRKEV
>Mature_167_residues
TKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFH
DIQSVLDWYQGQDKNLYILGGKQIFQAFEPYLDEVIVTHIHARVEGDTYFPEEFDLSLFETVSSKFYAKDEKNPYDFTIQ
YRKRKEV

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=153, Percent_Identity=33.9869281045752, Blast_Score=101, Evalue=3e-23,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19733; Mature: 19602

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPK
CCCEEEEEEECCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCC
RETLILTRNPEEKIDGVATFHDIQSVLDWYQGQDKNLYILGGKQIFQAFEPYLDEVIVTH
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
IHARVEGDTYFPEEFDLSLFETVSSKFYAKDEKNPYDFTIQYRKRKEV
HHEEECCCCCCCCCCCHHHHHHHHHHHEECCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
TKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPK
CCEEEEEEECCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCC
RETLILTRNPEEKIDGVATFHDIQSVLDWYQGQDKNLYILGGKQIFQAFEPYLDEVIVTH
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
IHARVEGDTYFPEEFDLSLFETVSSKFYAKDEKNPYDFTIQYRKRKEV
HHEEECCCCCCCCCCCHHHHHHHHHHHEECCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371341; 11463916 [H]