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The map label for this gene is hemN [H]

Identifier: 225859174

GI number: 225859174

Start: 1342434

End: 1343564

Strand: Reverse

Name: hemN [H]

Synonym: SP70585_1448

Alternate gene names: 225859174

Gene position: 1343564-1342434 (Counterclockwise)

Preceding gene: 225859175

Following gene: 225859173

Centisome position: 61.5

GC content: 42.71

Gene sequence:

>1131_bases
ATGCAGAAAAAACCAACGTCAGCCTATGTGCACATCCCATTTTGTACCCAGATTTGTTATTATTGTGATTTTTCAAAGGT
CTTCATCAAAAATCAGCCAGTCGACAGCTATTTAGAGCATCTGCTGGAAGAGTTTCGTTCTTATGATATTGAAAAGTTGT
CAACCCTTTATATCGGTGGTGGAACACCGACAGCCCTGTCGGCTCCGCAACTGGAGGTGTTACTGAATGGCTTGACTAAA
AACTTGGACTTGTCTGTCTTGGAAGAGTTGACCATTGAAGCCAATCCAGGCGATTTGGATGCGGATAAGATAGCTGTTTT
GAAAAATTCGGCTGTCAATCGTGTTTCGCTAGGTGTCCAGACCTTTGATGATAAGATGTTGAAAAAGATTGGGCGCAGTC
ATTTGGAGAAGGATATTTATGAAAATATCGATCGTCTGAAACTGGCTGGTTTTGACAACATCTCCATTGATTTGATTTAT
GCTCTGCCTGGTCAGACCATGGAGCAAGTAAAGGAAAATGTGGCTAAAGCCATTGGACTGGATATTCCCCACATGAGTTT
GTATAGTTTGATTTTAGAAAACCATACGGTCTTTATGAACCGGATGCGACGTGGGAAATTGCCTCTGCCTAAGGAGGAAC
TAGAAGCGGAGATGTTTGAGTACATCATTGCAGAGCTGGAGCGAGCGGGTTTTGAGCATTATGAGATTTCTAATTTCTCC
AAACCCGGCTTTGAAAGTCGTCATAATCTCATGTACTGGGACAATGCTGAATACTATGGTATTGGTGCAGGGGCATCTGG
TTATGTCAATGGAGTACGTTATAAAAATCATGGTCCCATTCGTCATTATCTCAGTGCGGTTGAGGAAGGCAATGCTTGTA
TTACAGAAGATCACCTGAGTCAAAAGGAGCAAATGGAAGAAGAAATGTTCTTGGGACTCCGCAAGAAATCCGGGGTTTCC
ATGGCGCGATTTGAGGAAAAATTTGGACAGTCTTTTGCTGGACTTTATGGAGAAATTGTCAGAGATTTGGTTCAACAAGG
CCTCATGCAGATTGAGGGTGACCACGTTCGCATGACAAAGAGAGGTCTCTTTTTGGGAGATACTGTAGCAGAACGATTTA
TTTTGGAGTAG

Upstream 100 bases:

>100_bases
ATATAACAGAAGAATAAACCATCACCATCTCCGGACGGACTATGTATCTGGGGATGGTGATTTTTATCTGGAATCTAGTC
TTTGTGGTATAATAATTACT

Downstream 100 bases:

>100_bases
GATCATGGGCTTAACTTATCAAATGAAAATGAAAATTCCTTTTGATATGGCTGATATGAACGGTCATATCAAACTTCCAG
ATGTGATTTTGCTGTCACTG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEEFRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTK
NLDLSVLEELTIEANPGDLDADKIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEMFEYIIAELERAGFEHYEISNFS
KPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKNHGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVS
MARFEEKFGQSFAGLYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE

Sequences:

>Translated_376_residues
MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEEFRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTK
NLDLSVLEELTIEANPGDLDADKIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEMFEYIIAELERAGFEHYEISNFS
KPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKNHGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVS
MARFEEKFGQSFAGLYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE
>Mature_376_residues
MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEEFRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTK
NLDLSVLEELTIEANPGDLDADKIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEMFEYIIAELERAGFEHYEISNFS
KPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKNHGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVS
MARFEEKFGQSFAGLYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=263, Percent_Identity=33.0798479087452, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1789325, Length=272, Percent_Identity=37.8676470588235, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI87082341, Length=385, Percent_Identity=25.4545454545455, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 42749; Mature: 42749

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEEFRSYDIEKLSTLYIGG
CCCCCCCCEEECHHHHHHHHHHCCHHHEECCCCHHHHHHHHHHHHHCCCHHHHEEEEECC
GTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDADKIAVLKNSAVNRVSLGVQ
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHH
TFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIYALPGQTMEQVKENVAKAIGL
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
DIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEMFEYIIAELERAGFEHYEISNFS
CCCHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCC
KPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKNHGPIRHYLSAVEEGNACITEDHLS
CCCCCCCCCEEEECCCCEEEECCCCCCCCCCCEECCCCCHHHHHHHHHCCCCEECHHHHH
QKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAGLYGEIVRDLVQQGLMQIEGDHVRMTK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
RGLFLGDTVAERFILE
CCCEECHHHHHHHCCC
>Mature Secondary Structure
MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEEFRSYDIEKLSTLYIGG
CCCCCCCCEEECHHHHHHHHHHCCHHHEECCCCHHHHHHHHHHHHHCCCHHHHEEEEECC
GTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDADKIAVLKNSAVNRVSLGVQ
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHH
TFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIYALPGQTMEQVKENVAKAIGL
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
DIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEMFEYIIAELERAGFEHYEISNFS
CCCHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCC
KPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKNHGPIRHYLSAVEEGNACITEDHLS
CCCCCCCCCEEEECCCCEEEECCCCCCCCCCCEECCCCCHHHHHHHHHCCCCEECHHHHH
QKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAGLYGEIVRDLVQQGLMQIEGDHVRMTK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
RGLFLGDTVAERFILE
CCCEECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]