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The map label for this gene is glnH [H]

Identifier: 225859159

GI number: 225859159

Start: 1330879

End: 1331694

Strand: Reverse

Name: glnH [H]

Synonym: SP70585_1433

Alternate gene names: 225859159

Gene position: 1331694-1330879 (Counterclockwise)

Preceding gene: 225859160

Following gene: 225859157

Centisome position: 60.96

GC content: 39.58

Gene sequence:

>816_bases
ATGAAATCAAAAAAATGGATATTTGTTTTATGTAATTTTCTTGCAAGTTTCTTCTTAGTAGCTTGCCAGTCAGGTTCTAA
TGGTTCTCAGTCTGCTGTGGATGCTATCAAACAAAAAGGGAAATTAGTTGTGGCAACCAGTCCTGACTATGCACCCTTTG
AATTTCAATCATTGGTTGATGGAAAGAACCAGGTAGTCGGTGCAGACATCGACATGGCTCAGGCTATCGCTGATGAACTT
GGGGTTAAGTTGGAAATCTTAAGCATGAGTTTTGACAATGTTTTGACCAGTCTTCAAACTGGTAAGGCTGACCTAGCAGT
TGCAGGAATTAGTGCTACTGACGAGAGAAAAGAAGTCTTTGATTTTTCAATCCCATACTATGAAAACAAGATTAGTTTCT
TGGTTCGTAAGGCTGATGTGGAAAAATACAAGGATTTAACTAGCCTAGAAAGTGCTAATATTGCAGCCCAAAAAGGGACT
GTTCCAGAATCAATGGTCAAGGCACAATTGCCAAAAGCTCAATTAACTTCCCTAACTAATATGGGTGAAGCAGTCAATGA
ATTGCAGGCTGGAAAAATAGATGCTGTTCATATGGATGAGCCTGTTGCACTTAGTTATGCTGCTAAAAACGCTGGCTTAG
CTGTCGCAACTGTCAGCTTGAAGATGAAGGACGGCGACGCCAATGCCGTTGCTCTTAGAAAAAATAGTGATGATTTGAAA
GAAGTGGTAGACAAGGTCATCCAAAAACTTAAGGATGAAGGAACTTACCAAAGCTATCTTGAAAAAGCAGCAAGTTTAAC
TGAAGTTGAAGAATAA

Upstream 100 bases:

>100_bases
GAACGAAAAAATATTTTTGAGATAAAAATTTAAAAAACACTTGACAAGTAACTTGGATATCGTTATAATTATACAAAATT
AACAGAGAGGTTGTTTATTT

Downstream 100 bases:

>100_bases
GAAAAAGCAGAGTTGGATGTCATTCCAATTCTGCTTTTAAATAGTTTAAAACATTTTCCAGATTGTCTAGGGACACCTTG
GCAAATTGATAAGGACAGGT

Product: amino acid ABC transporter, amino acid-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVVATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADEL
GVKLEILSMSFDNVLTSLQTGKADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
VPESMVKAQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATVSLKMKDGDANAVALRKNSDDLK
EVVDKVIQKLKDEGTYQSYLEKAASLTEVEE

Sequences:

>Translated_271_residues
MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVVATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADEL
GVKLEILSMSFDNVLTSLQTGKADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
VPESMVKAQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATVSLKMKDGDANAVALRKNSDDLK
EVVDKVIQKLKDEGTYQSYLEKAASLTEVEE
>Mature_271_residues
MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVVATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADEL
GVKLEILSMSFDNVLTSLQTGKADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
VPESMVKAQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATVSLKMKDGDANAVALRKNSDDLK
EVVDKVIQKLKDEGTYQSYLEKAASLTEVEE

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=222, Percent_Identity=34.6846846846847, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787088, Length=247, Percent_Identity=27.5303643724696, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787085, Length=219, Percent_Identity=29.2237442922374, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788228, Length=254, Percent_Identity=28.3464566929134, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788649, Length=226, Percent_Identity=26.9911504424779, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29234; Mature: 29234

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVVATSPDYAPFEFQSLVD
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHC
GKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGKADLAVAGISATDERKEVF
CCCCEEECCHHHHHHHHHHHCCEEEEEEECHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
DFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGTVPESMVKAQLPKAQLTSLTN
EECCCCHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHH
MGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATVSLKMKDGDANAVALRKNSDDLK
HHHHHHHHHCCCEEEEECCCCEEEEEHHCCCCEEEEEEEEEEECCCCCEEEEECCCHHHH
EVVDKVIQKLKDEGTYQSYLEKAASLTEVEE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVVATSPDYAPFEFQSLVD
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHC
GKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGKADLAVAGISATDERKEVF
CCCCEEECCHHHHHHHHHHHCCEEEEEEECHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
DFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGTVPESMVKAQLPKAQLTSLTN
EECCCCHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHH
MGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATVSLKMKDGDANAVALRKNSDDLK
HHHHHHHHHCCCEEEEECCCCEEEEEHHCCCCEEEEEEEEEEECCCCCEEEEECCCHHHH
EVVDKVIQKLKDEGTYQSYLEKAASLTEVEE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]