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The map label for this gene is mcrB [H]

Identifier: 225859045

GI number: 225859045

Start: 1221070

End: 1223001

Strand: Reverse

Name: mcrB [H]

Synonym: SP70585_1314

Alternate gene names: 225859045

Gene position: 1223001-1221070 (Counterclockwise)

Preceding gene: 225859046

Following gene: 225859044

Centisome position: 55.98

GC content: 33.28

Gene sequence:

>1932_bases
ATGAATAAAAGAAAAGTTAGTTTAGAAGATTTTTATAAATGGTATAGTCTAAATAAAGAAGAGTTATTAAATAAGGCAAC
TGTTGGTGAAAAGTTTAATGATAAATTAAAAGAAGAGTTTCTCCAGGAATGGCCTTTGGATAGGATTTTAACAATGTCAA
TCGATGAATATGTAATAGGAAAGGGACAGCAAAATAAGTCTTTATGCTACGCTCTTGAGAAGGGAAAATACAAAAATCTA
TTTCTTGGAATTTCTGGTGGCTCAGCTTCAAAATTTGGTATTTATTGGAATAAAAAAACAAACAAATATAAAGATCAAGC
TAATAATGAGATTTCAGAGTTGGATCAGCGATTTTCAAAATTAAAATCAGATTTGTATGAAATTATCAAAGAAGGTATTC
GTTTTAACTTTGAAAATCCTATTTTTGATATGAAAAGATCAACAAATGAATTTATTGGTCGTTCTGCTATGGTGACAAAA
TTACTTTGTATCTATTCTGAGGGAGATCCTTTCTTTGGTGTAAATATTAATAGTCAGAAAGAATTTTGGAACCACTTTGT
TTCTCAGACAAATCAAGGTGGACCTTATCTGCAAAATCATAAAATAATTGAACTGGTGTCCAAAACTTATCCTGAGTTGG
AGCCATCGAAAGTAGGAACTATGCTTTTTGAGTATTCTAAGCTTTTTATGGAAAATAAGGAAGACAATAGTACAATGGAT
TCATCAAACAATTTTCGTCATCAATTAACTCAATCTCTATTAAAGTCTCCAAACCTTATCCTTCGTGGTGCTCCTGGCAC
AGGAAAGACTTATCTTGCTAAAGAAATTGCCAAAGAATTAACGGATGGCAACGAAGACCAAATCGGATTTGTACAATTTC
ACCCATCATATGATTATACGGATTTTGTAGAAGGTTTAAGACCAGTATCAAATGGGGATGGAGCTATTGAGTTTAGGCTA
CAGGACGGTATTTTTAAAGATTTTTGTCAGAAAGCAAAAGAAACCCAATTGATTGGAGGACAAGATAATTTTGATGAGGC
TTGGGATTCTTACTTAGAATATATAAATGTTGCTGAAGAAAAAGAATATATAACAAAAACATCTTACTTATCTGTTAATA
GTAGACAAAATTTGTCAGTAAATTATGATAGTGGTGTTCCAGGATGGTCACTACCTAGCAAATATGTTTACGAGTTGTAT
AAAGATAAAAATTATAATAAGCAAGAATACTACAAAAGTGGTGGAAAAACTGTCCTAGAAACATTGAGAAAGAGATTTGG
TTTGAAAGACTATGTTTCCCCAACAGAAATTGATACTGATAAGAAATTCGTCTTCATCATCGATGAAATCAATCGTGGGG
AGATTTCTAAGATTTTTGGCGAACTCTTTTTCTCTATCGACCCTGGCTATCGTGGTGAAAAAGGAAGTGTTTCTACCCAA
TATGCAAATCTACACGAAACTGATGAAAAGTTCTATATCCCCGAAAATGTTTACATCATCGGAACTATGAATGATATTGA
TCGTTCAGTGGATACCTTTGATTTTGCTATGCGTCGTCGTTTTCGTTTTGTTGAAGTTACTGCCGAGGGTCAAGTTGGCA
TGTTGGGTAAAGAGCTGAATATCCATGCAGAAGAAGCAAAAATTCGTCTAAGAAACTTGAACGCTGCTATCGAAAATGTT
CAGGAATTAAACAGTCATTATCATATTGGACCAAGTTATTTTCTTAAGTTGAAGGATGTAGATTTTGACTATGAATTACT
CTGGTCTGATTATATTAAGCCTCTCCTAGAAGACTACTTGCGAGGTTCTTATGATGAGGTTGAAACTTTGGAAACTTTGA
AAAAAGCATTTGAACTGACAAATAATGAGCAAAAAGATCAGGCAGTAGCTGATGACAATGAAGGTGATGAAAACGATGAT
GCGGATTACTGA

Upstream 100 bases:

>100_bases
ATTCTTTTCTTTTTCTATAGGATATGGTATTCTATGTAGAAAATATATGTTAATAAGTAATGCCAATATTTAAACATCAT
TAGTAAAAGGAGTTAGATTG

Downstream 100 bases:

>100_bases
TAATCAACACAAGATTATTAAAGAAAAATTTGTTGAAGAATATCCTAAACTAAGCAATCTTCTTTTAGACAGAACCTTGG
AAAGTCTATCCCAAGATGAA

Product: hypothetical protein

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWPLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNL
FLGISGGSASKFGIYWNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
LLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKVGTMLFEYSKLFMENKEDNSTMD
SSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRL
QDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELY
KDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQ
YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLGKELNIHAEEAKIRLRNLNAAIENV
QELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDD
ADY

Sequences:

>Translated_643_residues
MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWPLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNL
FLGISGGSASKFGIYWNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
LLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKVGTMLFEYSKLFMENKEDNSTMD
SSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRL
QDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELY
KDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQ
YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLGKELNIHAEEAKIRLRNLNAAIENV
QELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDD
ADY
>Mature_643_residues
MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWPLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNL
FLGISGGSASKFGIYWNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
LLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKVGTMLFEYSKLFMENKEDNSTMD
SSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRL
QDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELY
KDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQ
YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLGKELNIHAEEAKIRLRNLNAAIENV
QELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDD
ADY

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=174, Percent_Identity=38.5057471264368, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 74609; Mature: 74609

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWPLDRILTMSIDEYVIG
CCCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHEEHHEHHHHHHC
KGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNKYKDQANNEISELDQRFSK
CCCCCCCCEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHH
LKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTKLLCIYSEGDPFFGVNINSQK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCHH
EFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKVGTMLFEYSKLFMENKEDNSTMD
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYT
CCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
DFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEE
HHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHH
KEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLE
HHHHHHHHEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHH
TLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQ
HHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEH
YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLGKELN
CCCCCCCCCCEECCCCEEEEEECHHHHHHHHHHHHHHHHCEEEEEEEECCCEEECCCCCC
IHAEEAKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYL
EEHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHEEEEECCCCCHHHHHHHHHHHHHHHHH
RGSYDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDDADY
CCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWPLDRILTMSIDEYVIG
CCCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHEEHHEHHHHHHC
KGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNKYKDQANNEISELDQRFSK
CCCCCCCCEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHH
LKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTKLLCIYSEGDPFFGVNINSQK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCHH
EFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKVGTMLFEYSKLFMENKEDNSTMD
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYT
CCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
DFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEE
HHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHH
KEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLE
HHHHHHHHEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHH
TLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQ
HHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEH
YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLGKELN
CCCCCCCCCCEECCCCEEEEEECHHHHHHHHHHHHHHHHCEEEEEEEECCCEEECCCCCC
IHAEEAKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYL
EEHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHEEEEECCCCCHHHHHHHHHHHHHHHHH
RGSYDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDDADY
CCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]