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The map label for this gene is ybjI [H]

Identifier: 225859040

GI number: 225859040

Start: 1213269

End: 1214063

Strand: Reverse

Name: ybjI [H]

Synonym: SP70585_1308

Alternate gene names: 225859040

Gene position: 1214063-1213269 (Counterclockwise)

Preceding gene: 225859041

Following gene: 225859039

Centisome position: 55.57

GC content: 40.13

Gene sequence:

>795_bases
ATGACAATTAAACTAGTAGCAACGGATATGGACGGAACCTTCCTAGATGGGAATGGACGCTTTGATATGGATCGTCTCAA
GTCTCTCTTGGCTTCCTACAAGGAAAAAGGGATTTACTTTGCGGTGGCTTCGGGTCGGGGATTTCTGTCTCTGGAAAAAT
TATTTGCTGGTGTTCGTGATGACATTATTTTCATCGCGGAAAATGGCAGTTTGGTAGAGTATCAAGGTCAGGACTTGTAT
GAAGCGACTATGTCTCGTGACTTTTATCTGGCAACTTTTGAAAAGCTGAAAACGTCACCTTATGTAGATATCAATAAACT
GCTCTTGACGGGTAAGAAGGGTTCATATGTTCTAGATACGGTTGATGAGACCTATTTGAAAGTGAGTCAGCATTATAATG
AAAATATCCAAAAAGTAGCGAGTTTGGAAGATATCACAGATGACATTTTCAAATTTACAACCAACTTCACAGAAGAAACG
CTGGAAGCTGGTGAAGCTTGGGTCAATGATAATGTCCCTGGTGTCAAGGCTATGACAACTGGCTTTGAATCTATTGATAT
TGTTCTGGACTATGTCGATAAGGGTGTAGCTATTGTTGAATTAGCTAAAAAACTTGGTATCACAATGGATCAGGTCATGG
CTTTTGGAGACAATCTTAATGACTTACATATGATGCAGGTTGTGGGACATCCTGTAGCTCCTGAAAATGCACGACCTGAA
ATTTTAGAATTAGCAAAGACTGTGATTGGTCACCATAAGGAACGGTCGGTTATAGCTTATATGGAGGGCTTATAA

Upstream 100 bases:

>100_bases
CCATGGCAGCGGCCGATTCTATCTATGGAGTGACCATGCAAGAATCGGGTGTTTCAAAGATTGTTTCAGTTAAGTTAAAA
GATTTAGAAAGTATTGAAGG

Downstream 100 bases:

>100_bases
TGGCAGATATAAAATTGATTGCATTGGACTTGGACGGGACCTTGCTGACTACTGATAAAAGGCTGACGGATCGTACCAAG
GAAACCTTGAAAGCTGCGCG

Product: Cof family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVASGRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLY
EATMSRDFYLATFEKLKTSPYVDINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDNLNDLHMMQVVGHPVAPENARPE
ILELAKTVIGHHKERSVIAYMEGL

Sequences:

>Translated_264_residues
MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVASGRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLY
EATMSRDFYLATFEKLKTSPYVDINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDNLNDLHMMQVVGHPVAPENARPE
ILELAKTVIGHHKERSVIAYMEGL
>Mature_263_residues
TIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVASGRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYE
ATMSRDFYLATFEKLKTSPYVDINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEETL
EAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDNLNDLHMMQVVGHPVAPENARPEI
LELAKTVIGHHKERSVIAYMEGL

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=259, Percent_Identity=31.2741312741313, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1787043, Length=248, Percent_Identity=28.6290322580645, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 29466; Mature: 29335

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVASGRGFLSLEKLFAGVRD
CEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHCCCCC
DIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYVDINKLLLTGKKGSYVLDT
CEEEEECCCCEEEECCCHHHHHHHCCCCHHHHHHHHCCCCCCEEEHEEEECCCCCEEEEC
VDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEETLEAGEAWVNDNVPGVKAMTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHC
GFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDNLNDLHMMQVVGHPVAPENARPE
CCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHH
ILELAKTVIGHHKERSVIAYMEGL
HHHHHHHHHCCCCCCCEEEEECCC
>Mature Secondary Structure 
TIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVASGRGFLSLEKLFAGVRD
EEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHCCCCC
DIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYVDINKLLLTGKKGSYVLDT
CEEEEECCCCEEEECCCHHHHHHHCCCCHHHHHHHHCCCCCCEEEHEEEECCCCCEEEEC
VDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEETLEAGEAWVNDNVPGVKAMTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHC
GFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDNLNDLHMMQVVGHPVAPENARPE
CCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHH
ILELAKTVIGHHKERSVIAYMEGL
HHHHHHHHHCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]