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The map label for this gene is metZ [H]

Identifier: 225858997

GI number: 225858997

Start: 1171573

End: 1172613

Strand: Reverse

Name: metZ [H]

Synonym: SP70585_1262

Alternate gene names: 225858997

Gene position: 1172613-1171573 (Counterclockwise)

Preceding gene: 225858998

Following gene: 225858996

Centisome position: 53.67

GC content: 42.84

Gene sequence:

>1041_bases
TTGAGGAAACCAGGGAACATTTATACTCGTATCACCAATCCTACAACAGCTGCCCTTGAAGGTGGTGTTGAAGCGCTAGC
AACAGCATCAGGTATGACTGCAGTGACTTATACGATTTTGGCGCTTGCCCATGCTGGTGACCATGTAGTGGCTGCTTCGA
CTATTTACGGTGGAACCTTCAATCTTTTGAAAGAACCCCTTCCTCGTTATGGTATCACAACAACCTTTGTCGATGTTGAT
AATTTGGAGGAAGTAGAAGCAGCTATCAAAGACAATACCAAGCTTGTCTTGATTGAAACCTTGGGTAACTCCTTGATTAA
TATTCCAGACCTGGAAAAACTGGCAGAGATTGCTCATAAACATCAAATCCCACTTGTGTCAGACAATACTTTTGCAATAC
CTTATTTGATTAACGTCTTCTCTCATGGCGTTGACATTGCCATTCACTCTGCGACTAAGTTTATCGGTGGGCATGGTACA
ACTATTGGAGGAATAATTGTCGATAGTGGTCGTTTTGACTGGACGGCTTCAGGGAAATTCCCTCAATTTGTTGACGAGGG
TCCAAGCTGCCACAATTTGAGTTATACTTGTGATGTGGGTGCAGCAGCCTTTATTATAGCTGTTCGAGTTCAATTGCTTC
GTGATACAGGTGCAGCCTTGTCACCATTCAATGCTTTCCTCTTGCTACAAAGACTTGAAACCTCTTCACTTCGTGTGGAA
CGCCATGTACAAAATGCTGAGACAATTGTTGATTTTCTTGTCAACCATCCTAAGGTAGAAAAGGTAAATTATCCAAAACT
TGCAGATAGTCCTTATCATGCCTTGGCTGAGAAATACTTGCCAAAAGATGTCGGTTCAATCTTTACCTTCCATGTCAAAG
GTGGCGAGGAAGAAGCACGCAAGGTCATTGATAATTTAGAAATCTTTTCTGACCTTGCAAACGCGGCAGATGCTAAATCG
CTTGTTGTCCATCCAGCAACAACCACTCACGGTCAATTGTCAGAAAAAGACCTAGAAGCAGCAGGTGTCACACCAAACTA
A

Upstream 100 bases:

>100_bases
TCAAGTTGTGGCTCCAGCTACTAAGTCTCGTGCAGTGCCGATTTATCAAACAACATCTTTTATTTTTGATGACACGTAGG
AAGGTGCTGATCTGTTTGCC

Downstream 100 bases:

>100_bases
ATTCGTTTGTCAATCGGTCTTGAAAATGTAGAAGATTTGATTGAAGACTTGCGCTTGGCCTTGGAAAAAATTTAAAGTAA
AAGAAGATAAACAGTGGGCT

Product: O-acetylhomoserine (thiol)-lyase (O-acetylhomoserinesulfhydrylase) (OAH sulfhydrylase) (Homocysteine synthase)

Products: L-methionine; acetate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAHAGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVD
NLEEVEAAIKDNTKLVLIETLGNSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETSSLRVE
RHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALAEKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKS
LVVHPATTTHGQLSEKDLEAAGVTPN

Sequences:

>Translated_346_residues
MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAHAGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVD
NLEEVEAAIKDNTKLVLIETLGNSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETSSLRVE
RHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALAEKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKS
LVVHPATTTHGQLSEKDLEAAGVTPN
>Mature_346_residues
MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAHAGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVD
NLEEVEAAIKDNTKLVLIETLGNSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETSSLRVE
RHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALAEKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKS
LVVHPATTTHGQLSEKDLEAAGVTPN

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=346, Percent_Identity=32.9479768786127, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI299473759, Length=339, Percent_Identity=29.4985250737463, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI25453487, Length=345, Percent_Identity=26.6666666666667, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1790375, Length=344, Percent_Identity=29.9418604651163, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789383, Length=310, Percent_Identity=24.5161290322581, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71980708, Length=350, Percent_Identity=32.2857142857143, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=349, Percent_Identity=30.945558739255, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17538055, Length=352, Percent_Identity=29.8295454545455, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323333, Length=360, Percent_Identity=46.3888888888889, Blast_Score=269, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6319307, Length=349, Percent_Identity=32.6647564469914, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=325, Percent_Identity=24.9230769230769, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=341, Percent_Identity=24.633431085044, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19922554, Length=350, Percent_Identity=30.5714285714286, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.49

Molecular weight: Translated: 37250; Mature: 37250

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAHAGDHVVAASTIYGGTF
CCCCCCEEEEECCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCH
NLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLGNSLINIPDLEKLAEIAHK
HHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
HQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGTTIGGIIVDSGRFDWTASGKF
CCCCEECCCCEEHHHHHHHHHCCCEEEEECHHHHHCCCCCEEEEEEEECCCCEEEECCCC
PQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETSSLRVE
CHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALAEKYLPKDVGSIFTFHVKGGEEEAR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHH
KVIDNLEIFSDLANAADAKSLVVHPATTTHGQLSEKDLEAAGVTPN
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAHAGDHVVAASTIYGGTF
CCCCCCEEEEECCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCH
NLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLGNSLINIPDLEKLAEIAHK
HHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
HQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGTTIGGIIVDSGRFDWTASGKF
CCCCEECCCCEEHHHHHHHHHCCCEEEEECHHHHHCCCCCEEEEEEEECCCCEEEECCCC
PQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETSSLRVE
CHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALAEKYLPKDVGSIFTFHVKGGEEEAR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHH
KVIDNLEIFSDLANAADAKSLVVHPATTTHGQLSEKDLEAAGVTPN
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: O-acetyl-L-homoserine; methanethiol

Specific reaction: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]