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The map label for this gene is lpdA

Identifier: 225858948

GI number: 225858948

Start: 1123860

End: 1125563

Strand: Reverse

Name: lpdA

Synonym: SP70585_1213

Alternate gene names: 225858948

Gene position: 1125563-1123860 (Counterclockwise)

Preceding gene: 225858949

Following gene: 225858947

Centisome position: 51.52

GC content: 42.61

Gene sequence:

>1704_bases
ATGGCCTTAGAAGTAATTATGCCAAAAGCCGGCGTGGATATGACAGAAGGACAAATCGTCCAATGGAATAAAAAAGTCGG
AGAATTTGTAAAAGAAGGAGAAATCCTTTTGGAAATCATGACTGATAAAGTCAGCATGGAATTGGAAGCCGAAGAAGATG
GGTACTTGATTGCCATTCTCAAAGGAGATGGTGAAACTGTCCCTGTAACGGAAGTTATCGGTTACCTTGGTGAAGAAAGG
GAAAACATCCCAACAGCTGGAGCAGCATCGCCAGAAGCTAGCCCTGTACCTGTAGCAAGTACCTCAAACGATGATGGTAA
GAGCGATGATGCTTTTGATATCGTTGTGATTGGTGGAGGTCCTGCTGGTTATGTTGCAGCCATTAAAGCTGCCCAACTCG
GCGGTAAGGTTGCCCTTGTTGAGAAATCTGAACTTGGTGGAACCTGCTTAAACCGTGGATGTATTCCAACCAAGACCTAC
CTTCATAACGCTGAAATTATTGAAAATATCGGTCATGCTGCAAATCGTGGTATCGTGATTGAAAATCCTAACTTCACTGT
TGATATGGAAAAACTTTTAGAAACTAAATCTAAAGTTGTTAATACTCTTGTTGGTGGTGTTGCAGGACTTCTTCGTAGCT
ACGGAGTTACTGTTCATAAAGGAATTGGTACAATCACTAAAGACAAGAACGTCTTGGTAAATGGTTCTGAATTGCTTGAA
ACCAAGAAAATCATTCTTGCTGGTGGTTCAAAAGTCAGCAAGATCAACGTCCCTGGTATGGAATCATCACTTGTCATGAC
TAGTGATGACATTCTTGAAATGAACGAAGTGCCAGAAAGCCTTGTTATCATCGGTGGTGGAGTTGTCGGTATCGAACTCG
GTCAGGCCTTCATGACATTTGGTTCAAAAGTGACTGTTATCGAAATGATGGACCGTATAGTTCCAGCTATGGATGCGGAA
GTTTCTAAGAACCTTCGCTTGATCCTTGAGCGTAAAGGAATGACCATCTTGACTGGTACTAAACTGCAAGAAATCATTGA
GGAAAATGGTCAACTTCGTATCAAGGTTGAAGGAAAAGACGATATCATCGCAAGCAAAGCTCTTCTTTCAATTGGTCGTA
TGCCAGACCTTGAAGGTATTGGAGAGGTTGAGTTTGAATTGGATCGTGGTTGTATCAAGGTCAACGAATACATGGAAACT
TCAGTTCCAGGCATTTATGCACCAGGTGACATCAACGGTACTAAGATGTTGGCTCACGCAGCTTTCCGCATGGGTGAAGT
TTCCGCTGAAAATGCCCTTAAAGGAAATCATGCAGTTGCCAAATTGAATTTGACTCCTGCAGCCATCTACACTCTCCCTG
AAGTAGCAGCAGTAGGTTTGACAGAAGAACAAGCCCGTGAGAAATACGATGTTGCCATCGGTAAGTTTAACTTTGCTGCT
AACGGTCGTGCTATTGCATCTGACGCAGCTCAAGGTTTCGTAAAAGTTATCGCTGATAAGAAATATGGAGAAATCCTTGG
TGTACACATTATTGGTCCTGCAGCCGCAGAATTAATAAACGAAGCATCAAGCATCATCGAAATGGAAATCACTGTTGAGG
AAATGCTGAAGACCATCCACGGACACCCAACTTACTCTGAAGTGATGTACGAAGCGTTTGCAGATGTTCTAGGAATGGCC
ATCCATTCACCTAAGAAAAAATAA

Upstream 100 bases:

>100_bases
GTTTATGAAGGACTTGAAAGAGTTGATTGAAACCCCAATCTCAATGTTGATTTAAGAGCAAGAGTATTCATTTTTAAGAT
ATAGATAAAGGAAGGAAGAC

Downstream 100 bases:

>100_bases
AACGAAGATAGATTAGACTGGAAGGTTATTTCCAGTCTCTATCATATAAAGTAAAGGGATATCATGAAATACATTATCAA
TCATTCAAATGACACTGCTT

Product: dihydrolipoyl dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEER
ENIPTAGAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTY
LHNAEIIENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGTITKDKNVLVNGSELLE
TKKIILAGGSKVSKINVPGMESSLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAE
VSKNLRLILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMET
SVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAA
NGRAIASDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMA
IHSPKKK

Sequences:

>Translated_567_residues
MALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEER
ENIPTAGAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTY
LHNAEIIENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGTITKDKNVLVNGSELLE
TKKIILAGGSKVSKINVPGMESSLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAE
VSKNLRLILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMET
SVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAA
NGRAIASDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMA
IHSPKKK
>Mature_566_residues
ALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERE
NIPTAGAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYL
HNAEIIENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGTITKDKNVLVNGSELLET
KKIILAGGSKVSKINVPGMESSLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEV
SKNLRLILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETS
VPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAAN
GRAIASDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAI
HSPKKK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=38.7234042553192, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=27.1334792122538, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI22035672, Length=461, Percent_Identity=29.5010845986985, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI33519430, Length=458, Percent_Identity=25.1091703056769, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI33519428, Length=458, Percent_Identity=25.1091703056769, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI33519426, Length=458, Percent_Identity=25.1091703056769, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI148277065, Length=458, Percent_Identity=25.1091703056769, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI148277071, Length=458, Percent_Identity=25.1091703056769, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI291045266, Length=461, Percent_Identity=24.7288503253796, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI291045268, Length=452, Percent_Identity=24.1150442477876, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1786307, Length=448, Percent_Identity=38.1696428571429, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI87082354, Length=459, Percent_Identity=27.6688453159041, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=27.8260869565217, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789915, Length=430, Percent_Identity=27.6744186046512, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789065, Length=297, Percent_Identity=24.5791245791246, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=473, Percent_Identity=39.7463002114165, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17557007, Length=480, Percent_Identity=26.875, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=450, Percent_Identity=27.3333333333333, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=450, Percent_Identity=27.3333333333333, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=478, Percent_Identity=24.6861924686192, Blast_Score=104, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=484, Percent_Identity=38.4297520661157, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=29.1139240506329, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=28.0777537796976, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=79, Percent_Identity=39.2405063291139, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21358499, Length=460, Percent_Identity=42.1739130434783, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=26.3713080168776, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640551, Length=501, Percent_Identity=24.9500998003992, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640553, Length=479, Percent_Identity=24.6346555323591, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24640549, Length=466, Percent_Identity=24.6781115879828, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 60344; Mature: 60213

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAIL
CCEEEEECCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCEEEEEE
KGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKSDDAFDIVVIGGG
ECCCCEECHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCC
PAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEIIENIGHAANRGIVI
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCHHHHCCHHHHHHHCHHHCCCEEE
ENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGTITKDKNVLVNGSELLE
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCEEECCHHHHC
TKKIILAGGSKVSKINVPGMESSLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTF
EEEEEEECCCEEEEEECCCCCCEEEEECCCHHHHHCCCCEEEEEECCEEEEHHHHHHHHC
GSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGTKLQEIIEENGQLRIKVEGKD
CCCCHHHHHHHHHHHHCCHHHHCCEEEEEEECCCEEEECHHHHHHHHCCCEEEEEECCCC
DIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIYAPGDINGTKMLAHA
CHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEHHHHHHCCCCEECCCCCCHHHHHHHH
AFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAA
HHHHCCCCHHHCCCCCCEEEEEECCHHHHHCCCHHHHCCCCHHHHHHHHCEEEEEEEECC
NGRAIASDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIH
CCCEEHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHEEEEEEHHHHHHHHC
GHPTYSEVMYEAFADVLGMAIHSPKKK
CCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAIL
CEEEEECCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCEEEEEE
KGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKSDDAFDIVVIGGG
ECCCCEECHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCC
PAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEIIENIGHAANRGIVI
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCHHHHCCHHHHHHHCHHHCCCEEE
ENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGTITKDKNVLVNGSELLE
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCEEECCHHHHC
TKKIILAGGSKVSKINVPGMESSLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTF
EEEEEEECCCEEEEEECCCCCCEEEEECCCHHHHHCCCCEEEEEECCEEEEHHHHHHHHC
GSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGTKLQEIIEENGQLRIKVEGKD
CCCCHHHHHHHHHHHHCCHHHHCCEEEEEEECCCEEEECHHHHHHHHCCCEEEEEECCCC
DIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIYAPGDINGTKMLAHA
CHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEHHHHHHCCCCEECCCCCCHHHHHHHH
AFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAA
HHHHCCCCHHHCCCCCCEEEEEECCHHHHHCCCHHHHCCCCHHHHHHHHCEEEEEEEECC
NGRAIASDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIH
CCCEEHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHEEEEEEHHHHHHHHC
GHPTYSEVMYEAFADVLGMAIHSPKKK
CCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2832161; 2404760; 1880807 [H]