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The map label for this gene is insI1 [C]

Identifier: 225858837

GI number: 225858837

Start: 999627

End: 1000580

Strand: Reverse

Name: insI1 [C]

Synonym: SP70585_1087

Alternate gene names: 225858837

Gene position: 1000580-999627 (Counterclockwise)

Preceding gene: 225858838

Following gene: 225858808

Centisome position: 45.8

GC content: 38.57

Gene sequence:

>954_bases
ATGCAAGAACATTATACCCCAAAAGGGAAACATTTGACAATAGATAACCGTCGCTTGATTAAGCGGTGGAAGAATGAAAA
TAAGTCCAATCGTGAAATTGCAGGCTTGTTAGGAAAGGCGCCTCAAACGATTCATAATGAAGTCAAAAGAGGTACAACTT
TACAACAAGTGAGAAAAGGGCTATACAAAAAGGTCTATTCTGCCGATTACGCACAAACTGTTTACCAATTCAATCGAAAA
CGGTCGGTGAAAAAGTTAATTTTAACAAAGGAAATCAGAGAGAAGATCTTACACTATCATAAGCAAAAATTTTCGCCTGA
AATGATGGTTAACAAGAAGCAAGTGAAAGTTGGTATTTCAACCATCTACTACTGGTTTCATAATGGTCATTTAGGATTGA
CGAAGGCCGACATGCTTTATCCCAGAAAAAGGAAAGGTGTCAAGAAGCAAGCTAGTCCGAACTTTAAGCCGGCAGGTAAA
TCTATCGAAGAACGTCCTGACGTTATTAATCTTCGCTTGGAAAATGGTCATTATGAAATTGATACCGTCCTACTGACTAA
GATAAAAAATTATTGCCTGTTAGTCTTAACCGACCGGCGGAGCAGACACCAAATTATAAGGTTAATTCCAAATAAAACTG
CTGAATCTGTCAATCAGGCGCTTACGTTACTATTAGGGGAGCATCGTATTCTGTCCATTACTGCAGATAATGGTTCGGAG
TTCAAACGATTGTCTGAGGTATTTCCTGAGGAACATATCTACTACGCACATGCTTACTCTTCATGGGAGAGAGGTTCAAA
TGAAAATCATAATCGATTAATTCGGGGATGGTTACCTAAAGGAACCAAGAAAACGACTCCGAAAGAAGTAGCTTTTATCG
AAAATTGGATTAACAACTACCCTAAAAAATGCTTGGACTACAAGTCGCCAAGTGAATTTCTTTTGGGTGGCTAA

Upstream 100 bases:

>100_bases
TCAAGGGTTCCTGTTGTCAAATGAAATGTGATTTAAGGTATAAGAAAACACCTCTGTGCTATACTTGTTGTTCACCACAA
ACACAAGGAAAGGCACAGAG

Downstream 100 bases:

>100_bases
CTTCAACTTGAAATTTGGGCTTTGTCTCTATGCTAAAGGAATCCGCTATAAATACAAGTACTATAGCCGTCGGCATTCCT
CATTAACACGACCCAATTTG

Product: transposase

Products: NA

Alternate protein names: Transposon TN4551 [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MQEHYTPKGKHLTIDNRRLIKRWKNENKSNREIAGLLGKAPQTIHNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRK
RSVKKLILTKEIREKILHYHKQKFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVNQALTLLLGEHRILSITADNGSE
FKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRGWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG

Sequences:

>Translated_317_residues
MQEHYTPKGKHLTIDNRRLIKRWKNENKSNREIAGLLGKAPQTIHNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRK
RSVKKLILTKEIREKILHYHKQKFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVNQALTLLLGEHRILSITADNGSE
FKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRGWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG
>Mature_317_residues
MQEHYTPKGKHLTIDNRRLIKRWKNENKSNREIAGLLGKAPQTIHNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRK
RSVKKLILTKEIREKILHYHKQKFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVNQALTLLLGEHRILSITADNGSE
FKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRGWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790736, Length=334, Percent_Identity=31.437125748503, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787671, Length=334, Percent_Identity=31.437125748503, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1786450, Length=334, Percent_Identity=31.437125748503, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 37123; Mature: 37123

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEHYTPKGKHLTIDNRRLIKRWKNENKSNREIAGLLGKAPQTIHNEVKRGTTLQQVRKG
CCCCCCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
LYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQKFSPEMMVNKKQVKVGIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHEEEHE
TIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGKSIEERPDVINLRLENGHYEI
EEEEEEECCCCCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCEEH
DTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVNQALTLLLGEHRILSITADNGSE
HHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHH
FKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRGWLPKGTKKTTPKEVAFIENWINNY
HHHHHHHCCHHCEEEEECHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHH
PKKCLDYKSPSEFLLGG
HHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MQEHYTPKGKHLTIDNRRLIKRWKNENKSNREIAGLLGKAPQTIHNEVKRGTTLQQVRKG
CCCCCCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
LYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQKFSPEMMVNKKQVKVGIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHEEEHE
TIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGKSIEERPDVINLRLENGHYEI
EEEEEEECCCCCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCEEH
DTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVNQALTLLLGEHRILSITADNGSE
HHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHH
FKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRGWLPKGTKKTTPKEVAFIENWINNY
HHHHHHHCCHHCEEEEECHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHH
PKKCLDYKSPSEFLLGG
HHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2820936 [H]