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The map label for this gene is trmE

Identifier: 225858807

GI number: 225858807

Start: 971951

End: 973324

Strand: Reverse

Name: trmE

Synonym: SP70585_1055

Alternate gene names: 225858807

Gene position: 973324-971951 (Counterclockwise)

Preceding gene: 225858808

Following gene: 225858803

Centisome position: 44.55

GC content: 44.69

Gene sequence:

>1374_bases
ATGATTACACGTGAATTTGATACCATCGCTGCTATCTCTACTCCACTAGGTGAAGGGGCTATTGGTATTGTCCGCCTGAG
CGGAACAGACAGTTTTGCTATTGCGCAAAAGATTTTTAAAGGAAAAGACTTGAACAAGGTTGCCAGCCACACTCTCAACT
ACGGTCACATTATTGATCCTCTGACTGGTAAAGTCATGGACGAGGTTATGGTTGGGGCTATGAAGTCTCCAAAGACCTTC
ACTCGTGAGGATATTATCGAGATTAACACCCACGGTGGGATTGCGGTGACCAATGAAATTCTCCAGCTAGCTATTCGTGA
AGGGGCTCGGTTGGCAGAACCTGGTGAATTTACCAAACGTGCTTTTTTAAACGGTCGCGTAGACTTGACACAGGCAGAGG
CTGTGATGGATATCATCCGTGCCAAGACTGACAAGGCCATGAACATTGCGGTCAAACAATTAGACGGCTCCCTTTCTGAC
CTCATTAACAATACCCGTCAAGAAATCCTCAATACACTTGCCCAAGTTGAGGTCAATATCGACTATCCTGAATATGATGA
TGTTGAGGAAGCTACTACTGCCGTTGTTCGTGAGAAGACTATGGAGTTTGAGCAATTGCTAACCAATCTTCTTAGAACAG
CCCGTCGCGGCAAAATCCTTCGTGAAGGAATTTCAACAGCTATCATCGGACGTCCCAACGTTGGGAAATCCAGCCTTCTC
AACAACCTCTTGCGTGAGGACAAGGCTATCGTAACAGATATCGCTGGGACAACACGAGATGTCATCGAAGAGTACGTCAA
CATCAATGGTGTACCTCTCAAATTGATTGATACAGCCGGTATTCGTGAAACAGATGACACAGTGGAACAAATCGGTGTTG
AGCGTTCGAAAAAAGCCCTCAAGGAAGCCGACTTGGTTCTACTAGTGCTAAATGCCAGTGAACCACTGACTGCGCAAGAC
AGACAACTTCTTGAAATTAGCCAAGATACCAATCGCATTATTCTACTTAATAAAACCGACCTGCCAGAAACGATTGAAAC
TTCGAAACTACCTGAAGACGTTATCCGTATTTCAGTCCTTAAAAACCAAAACATCGACAAGATTGAAGAGCGAATCAACA
ACCTCTTCTTTGAAAATGCTGGCTTGGTCGAGCAAGATGCTACCTACTTGTCAAACGCCCGTCACATTTCCCTGATTGAA
AAAGCAGTTGAAAGCCTACAAGCCGTTAATCAAGGTCTTGAGCTGGGGATGCCAGTTGATTTGCTTCAAGTTGACTTGAC
TCGTACTTGGGAAATCCTCGGAGAAATCACTGGGGATGCTGCTCCAGATGAACTTATTACCCAACTCTTTAGCCAATTCT
GTTTAGGAAAATAA

Upstream 100 bases:

>100_bases
TCCAAGTAGCATTCATTGAAGAAATATCCTAAATTTGTTACAATTTGAAAAGAAACTTGGAGAATTTCCAAGAAAAGAGC
TATTAATTAAAGGAAACATT

Downstream 100 bases:

>100_bases
GAAAAATCCATGATCCTTCATTCGGTCATGGATTTTATTGTCTTTATTAGTAATCTGGTCTTAAGACCCCTGTTACAGTT
GCCTTAGTTGCTTCGTAGTC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDLNKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTF
TREDIIEINTHGGIAVTNEILQLAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGKILREGISTAIIGRPNVGKSSLL
NNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDTVEQIGVERSKKALKEADLVLLVLNASEPLTAQD
RQLLEISQDTNRIILLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIE
KAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK

Sequences:

>Translated_457_residues
MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDLNKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTF
TREDIIEINTHGGIAVTNEILQLAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGKILREGISTAIIGRPNVGKSSLL
NNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDTVEQIGVERSKKALKEADLVLLVLNASEPLTAQD
RQLLEISQDTNRIILLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIE
KAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
>Mature_457_residues
MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDLNKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTF
TREDIIEINTHGGIAVTNEILQLAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGKILREGISTAIIGRPNVGKSSLL
NNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDTVEQIGVERSKKALKEADLVLLVLNASEPLTAQD
RQLLEISQDTNRIILLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIE
KAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=34.468085106383, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=33.9869281045752, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=32.8657314629259, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI2367268, Length=460, Percent_Identity=34.1304347826087, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=41.8032786885246, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=463, Percent_Identity=31.1015118790497, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=31.0344827586207, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=30.6553911205074, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50478; Mature: 50478

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDLNKVASHTLNYGHIIDP
CCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
LTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQLAIREGARLAEPGEFTKR
HHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSDLINNTRQEILNTLAQVEVNI
HHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEC
DYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGKILREGISTAIIGRPNVGKSSLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHH
NNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDTVEQIGVERSKKAL
HHHHHHCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCHHHHHHHH
KEADLVLLVLNASEPLTAQDRQLLEISQDTNRIILLNKTDLPETIETSKLPEDVIRISVL
HHCCEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHH
KNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIEKAVESLQAVNQGLELGMPVD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
LLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDLNKVASHTLNYGHIIDP
CCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
LTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQLAIREGARLAEPGEFTKR
HHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSDLINNTRQEILNTLAQVEVNI
HHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEC
DYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGKILREGISTAIIGRPNVGKSSLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHH
NNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDTVEQIGVERSKKAL
HHHHHHCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCHHHHHHHH
KEADLVLLVLNASEPLTAQDRQLLEISQDTNRIILLNKTDLPETIETSKLPEDVIRISVL
HHCCEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHH
KNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIEKAVESLQAVNQGLELGMPVD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
LLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA