BacMap

Definition	Brucella melitensis ATCC 23457 chromosome chromosome I, complete sequence.
Accession	NC_012441
Length	2,125,701

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The map label for this gene is fadB1 [H]

Identifier: 225853609

GI number: 225853609

Start: 2124120

End: 2124893

Strand: Direct

Name: fadB1 [H]

Synonym: BMEA_A2245

Alternate gene names: 225853609

Gene position: 2124120-2124893 (Clockwise)

Preceding gene: 225853608

Following gene: 225853610

Centisome position: 99.93

GC content: 60.21

Gene sequence:

>774_bases
ATGGCCTATGAAACAATCATCGTTGAGACGCGTGACAGCGTTGGTCTCATCCGTCTCAACCGTCCGCAGGCGCTCAACGC
GCTCAATCGCGCCGTGCTTGAGGAGCTGACCGATGCGCTTGCCGCCTTCGACGCGGATGGCAAGGTCGGCGCCGTGGTGC
TGACCGGCTCGGAAAAGGCTTTCGCCGCCGGGGCCGACATCAAGGAAATGCAGTCGATCAATTTCGTCGATGCCTATTTG
CAGGACATGTTCGCCGACTGGCAGAAGGTGGATCGTATCCGCAAGCCGATTATCGCGGCTGTTTCGGGCTATGCGCTGGG
CGGCGGCTGCGAACTCGCCATGATGTGCGATTTCATCATCGCGGGCAGCAATGCGAAATTCGGCCAGCCGGAAATCACGC
TGGGCGCCATTCCTGGCATGGGCGGCTCGCAGCGCCTGACGCGCTATGTCGGCAAGTCCAAGGCTATGGATATGTGCCTG
ACGGGCCGCATGATGGATGCAGCCGAAGCGGAGCGTTGCGGCCTTGTTTCCCGCGTTGTCGCACCGGAGGCTCTTCTGGA
AGAGGCACTGAAAGCAGCCGAACGCATCGCATCCTTCTCGCGCGTCTCGGTCTATATGGCCAAGGAAGCGGTCAACCGCG
CCTATGAAACGACGCTGGACGAGGGCCTGCGTTTCGAGCGCCGCCTGTTCCATTCGCTTTTCGCGACCGAAGACCAGAAG
GAAGGCATGTCCGCTTTCGTTGATAAACGTACCCCTTCCTTCAAGAACCGCTGA

Upstream 100 bases:

>100_bases
CCAATTTGCGCCACGGAATTTTAAGGCGGGGCAGCCTTGCCGGCACATTATCCCGGATGAGCCGGGCTGTCACGCGCTGA
TGAAGGAGGAACGGGTTTAC

Downstream 100 bases:

>100_bases
AGAACAGAGATGTCGGGGCGTTTCCCTCCGGCGTGAAACGGCTTTCGGATGGGATGCGCGTCGACGGCAGGAAGTGTTCC
AGAATCGTTGATGGTCTTTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MAYETIIVETRDSVGLIRLNRPQALNALNRAVLEELTDALAAFDADGKVGAVVLTGSEKAFAAGADIKEMQSINFVDAYL
QDMFADWQKVDRIRKPIIAAVSGYALGGGCELAMMCDFIIAGSNAKFGQPEITLGAIPGMGGSQRLTRYVGKSKAMDMCL
TGRMMDAAEAERCGLVSRVVAPEALLEEALKAAERIASFSRVSVYMAKEAVNRAYETTLDEGLRFERRLFHSLFATEDQK
EGMSAFVDKRTPSFKNR

Sequences:

>Translated_257_residues
MAYETIIVETRDSVGLIRLNRPQALNALNRAVLEELTDALAAFDADGKVGAVVLTGSEKAFAAGADIKEMQSINFVDAYL
QDMFADWQKVDRIRKPIIAAVSGYALGGGCELAMMCDFIIAGSNAKFGQPEITLGAIPGMGGSQRLTRYVGKSKAMDMCL
TGRMMDAAEAERCGLVSRVVAPEALLEEALKAAERIASFSRVSVYMAKEAVNRAYETTLDEGLRFERRLFHSLFATEDQK
EGMSAFVDKRTPSFKNR
>Mature_256_residues
AYETIIVETRDSVGLIRLNRPQALNALNRAVLEELTDALAAFDADGKVGAVVLTGSEKAFAAGADIKEMQSINFVDAYLQ
DMFADWQKVDRIRKPIIAAVSGYALGGGCELAMMCDFIIAGSNAKFGQPEITLGAIPGMGGSQRLTRYVGKSKAMDMCLT
GRMMDAAEAERCGLVSRVVAPEALLEEALKAAERIASFSRVSVYMAKEAVNRAYETTLDEGLRFERRLFHSLFATEDQKE
GMSAFVDKRTPSFKNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=56.9767441860465, Blast_Score=304, Evalue=5e-83,
Organism=Homo sapiens, GI20127408, Length=222, Percent_Identity=34.2342342342342, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=35.2941176470588, Blast_Score=117, Evalue=7e-27,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=34.251968503937, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI70995211, Length=265, Percent_Identity=30.188679245283, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=31.8897637795276, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI150378541, Length=243, Percent_Identity=31.6872427983539, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI37594471, Length=233, Percent_Identity=27.8969957081545, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI37594469, Length=233, Percent_Identity=27.8969957081545, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI25453481, Length=271, Percent_Identity=25.4612546125461, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI51538573, Length=271, Percent_Identity=25.4612546125461, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4757968, Length=272, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI49355787, Length=272, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4757966, Length=260, Percent_Identity=25.7692307692308, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI51538581, Length=260, Percent_Identity=25.7692307692308, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=25.2173913043478, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=25.2173913043478, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221136756, Length=270, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI221136753, Length=270, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI221307494, Length=270, Percent_Identity=25.9259259259259, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI45643119, Length=252, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI260275230, Length=252, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI260274832, Length=228, Percent_Identity=25.4385964912281, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI261878539, Length=93, Percent_Identity=41.9354838709677, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI157694520, Length=191, Percent_Identity=26.7015706806283, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI116235487, Length=213, Percent_Identity=30.0469483568075, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=48.2213438735178, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=34.6153846153846, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1788597, Length=269, Percent_Identity=32.3420074349442, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787660, Length=267, Percent_Identity=30.7116104868914, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=29.9625468164794, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790281, Length=186, Percent_Identity=32.258064516129, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788682, Length=200, Percent_Identity=32, Blast_Score=91, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=259, Percent_Identity=54.4401544401544, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=53.4979423868313, Blast_Score=270, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17540714, Length=226, Percent_Identity=34.5132743362832, Blast_Score=136, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17534483, Length=261, Percent_Identity=31.4176245210728, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=33.201581027668, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=230, Percent_Identity=32.1739130434783, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17536985, Length=271, Percent_Identity=28.7822878228782, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=225, Percent_Identity=30.2222222222222, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17508953, Length=225, Percent_Identity=30.2222222222222, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=225, Percent_Identity=30.2222222222222, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144160, Length=207, Percent_Identity=32.3671497584541, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=207, Percent_Identity=32.3671497584541, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=246, Percent_Identity=28.0487804878049, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=22.9249011857708, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=30.0518134715026, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=61.4754098360656, Blast_Score=310, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=61.4754098360656, Blast_Score=310, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=36.5079365079365, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=35.6807511737089, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=31.25, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=28.3524904214559, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19920382, Length=239, Percent_Identity=32.2175732217573, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24654903, Length=181, Percent_Identity=28.7292817679558, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24583077, Length=221, Percent_Identity=33.0316742081448, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583079, Length=221, Percent_Identity=33.0316742081448, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921000, Length=221, Percent_Identity=33.0316742081448, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571730, Length=177, Percent_Identity=26.5536723163842, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28571729, Length=177, Percent_Identity=26.5536723163842, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19921018, Length=210, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583165, Length=207, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28043; Mature: 27911

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYETIIVETRDSVGLIRLNRPQALNALNRAVLEELTDALAAFDADGKVGAVVLTGSEKA
CCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FAAGADIKEMQSINFVDAYLQDMFADWQKVDRIRKPIIAAVSGYALGGGCELAMMCDFII
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHH
AGSNAKFGQPEITLGAIPGMGGSQRLTRYVGKSKAMDMCLTGRMMDAAEAERCGLVSRVV
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
APEALLEEALKAAERIASFSRVSVYMAKEAVNRAYETTLDEGLRFERRLFHSLFATEDQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EGMSAFVDKRTPSFKNR
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AYETIIVETRDSVGLIRLNRPQALNALNRAVLEELTDALAAFDADGKVGAVVLTGSEKA
CCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FAAGADIKEMQSINFVDAYLQDMFADWQKVDRIRKPIIAAVSGYALGGGCELAMMCDFII
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHH
AGSNAKFGQPEITLGAIPGMGGSQRLTRYVGKSKAMDMCLTGRMMDAAEAERCGLVSRVV
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
APEALLEEALKAAERIASFSRVSVYMAKEAVNRAYETTLDEGLRFERRLFHSLFATEDQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EGMSAFVDKRTPSFKNR
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]