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The map label for this gene is pnp [H]

Identifier: 225853594

GI number: 225853594

Start: 2109483

End: 2111627

Strand: Direct

Name: pnp [H]

Synonym: BMEA_A2229

Alternate gene names: 225853594

Gene position: 2109483-2111627 (Clockwise)

Preceding gene: 225853593

Following gene: 225853598

Centisome position: 99.24

GC content: 57.72

Gene sequence:

>2145_bases
ATGTTCAATACCCATAAAGTTGAAATCGAATGGGGCGGACGTCCGCTCACACTCGAAACCGGCAAGATTGCCCGTCAGGC
TGACGGCGCTGTTCTCGCCACCTATGGCGAAACCGCCGTTCTCGCAACTGTCGTTTCTGCCAAGGAACCGAAGCCGGGCC
AGGACTTCTTCCCGCTGACCGTCAATTATCAGGAAAAGACCTATGCAGCCGGCAAGATTCCCGGCGGCTATTTCAAGCGT
GAAGGCCGTCCGAGCGAAAACGAAACCCTCGTTTCGCGCCTGATCGACCGTCCGATCCGCCCGCTTTTCGTTGACGGCTA
CAAGAACGACACGCAGGTCGTCATTACCGTTCTCCAGCACGATCTGGAAAACAACCCGGACATCCTGTCAATGGTTGCGG
CATCTGCCGCCCTCACCATTTCGGGCGTGCCTTTCATGGGTCCGATCAGCGGCGCGCGCGTTGGTTATATCGATGGCGAA
TATGTCCTGAACCCGAATATCGATGAAATGCCGGAATCCAAGCTCGATCTGGTGGTTGCCGGTACGTCGGAAGCGGTTCT
GATGGTCGAATCGGAAGCGCAGGAACTGCCCGAAGATGTCATGCTCGGCGCCGTCATGTTTGGCCACAAGAGCTTCCAGC
CGGTGATCGATGCGATCATCAAGCTTGCCGAAGTTGCCGCCAAGGAACCGCGTGACTTCCAGCCGGAAGACCTGTCGGAA
CTGGAAGCCAAGGTGCTGGCCGTTGTGGAAAACGACCTGCGCGAAGCTTACAAGATCACTGAAAAGCAGGCGCGCTACGC
AGCCGTTGATGCCGCCAAGGCCAAAGCCGAGGAACATTTCTTCCCCGAAGGCGTGGAAGAAACCGAAATGTCGGCGGAAC
AGTTCGCGACCATTTTCAAGCATCTGCAGGCCAAGATCGTTCGCTGGAACATTCTCGACACGGGCAACCGTATCGATGGC
CGTGACCTTTCGACCGTTCGTCCGATCGTTTCGGAAGTCGGCATCCTGCCGCGCACCCACGGTTCCGCGCTCTTCACCCG
CGGTGAAACGCAGGCCATCGTCGTTGCCACGCTCGGCACCGGCGAAGACGAACAGATGATCGATGCGCTGACGGGAACCT
ACAAGGAATCCTTCATGCTGCATTACAATTTCCCGCCCTATTCGGTCGGTGAAACGGGCCGCATGGGTTCTCCGGGCCGC
CGCGAAATCGGCCATGGCAAGCTTGCCTGGCGCGCAATCCATCCGATGCTGCCTGCCGCCGAACAGTTCCCCTATACGAT
CCGCGCTGTTTCCGAAATCACGGAATCGAACGGCTCTTCCTCGATGGCAACCGTTTGCGGCACCTCGCTGGCGCTGATGG
ATGCCGGTGTGCCGATCGTGCGCCCCGTCGCCGGTATCGCCATGGGTCTCATCAAGGAAGGCGAACGTTTCGCCGTTCTT
TCCGACATTCTCGGCGATGAAGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGTACCGAATTCGGCATCACATCGCT
ACAGATGGACATCAAGATCGACGGTATCACCGAAGAGATCATGAAGGTCGCTCTGGAACAGGCAAAGGGTGGCCGCGTTC
ACATTCTCGGCGAAATGGCCAAGGCCATCTCCTCCTCGCGCGCTGAACTGGGCGAGTTTGCCCCGCGCATCGAAGTGATG
AACATTCCGACCGACAAGATCCGCGACGTGATTGGCTCCGGCGGCAAGGTTATCCGCGAGATCGTGGAAAAGACCGGCGC
CAAGATCAACATCGAGGACGATGGTACGGTCAAGATCGCGTCGTCGAACGGCAAGGAAATCGAAGCCGCCAAGAAGTGGA
TTCACTCCATTGTGGCTGAACCGGAAGTCGGCGAAATCTATGAAGGCACGGTCGTCAAGACCGCCGATTTCGGCGCTTTC
GTGAACTTCTTCGGCCCGCGTGACGGTCTGGTTCATATCTCGCAGCTCGCTGCCGACCGTGTTGCCAAGACCACCGATGT
GGTCAAGGAAGGCCAGAAGGTCTGGGTCAAGCTCATGGGCTTCGACGAACGCGGCAAGGTTCGCCTGTCGATGAAGGTCG
TCGATCAGGAAACCGGCAAGGAAATCGTTGCTGAAAAGAAGAAGGAAGAAGTTGACGCTGAATAA

Upstream 100 bases:

>100_bases
CTTCCCGTTGTCTTGCCCGTGGCCTGTCCGCCAACCCGGCAACGCTTTCATGCGCTTTTGTCTAGCGCATTTTCACCGCG
TTGCCACATGAGGACAAGAT

Downstream 100 bases:

>100_bases
GCGTTGCATCTCTTCTGAAAACCGGAAAGCGCGCCTTTTGGCGCGCTTTTTGTTATTTCAGGGGATTGAGCGGACTTTTC
GGAAGCGATCTTGCGGGGTA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MFNTHKVEIEWGGRPLTLETGKIARQADGAVLATYGETAVLATVVSAKEPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSENETLVSRLIDRPIRPLFVDGYKNDTQVVITVLQHDLENNPDILSMVAASAALTISGVPFMGPISGARVGYIDGE
YVLNPNIDEMPESKLDLVVAGTSEAVLMVESEAQELPEDVMLGAVMFGHKSFQPVIDAIIKLAEVAAKEPRDFQPEDLSE
LEAKVLAVVENDLREAYKITEKQARYAAVDAAKAKAEEHFFPEGVEETEMSAEQFATIFKHLQAKIVRWNILDTGNRIDG
RDLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGTGEDEQMIDALTGTYKESFMLHYNFPPYSVGETGRMGSPGR
REIGHGKLAWRAIHPMLPAAEQFPYTIRAVSEITESNGSSSMATVCGTSLALMDAGVPIVRPVAGIAMGLIKEGERFAVL
SDILGDEDHLGDMDFKVAGTEFGITSLQMDIKIDGITEEIMKVALEQAKGGRVHILGEMAKAISSSRAELGEFAPRIEVM
NIPTDKIRDVIGSGGKVIREIVEKTGAKINIEDDGTVKIASSNGKEIEAAKKWIHSIVAEPEVGEIYEGTVVKTADFGAF
VNFFGPRDGLVHISQLAADRVAKTTDVVKEGQKVWVKLMGFDERGKVRLSMKVVDQETGKEIVAEKKKEEVDAE

Sequences:

>Translated_714_residues
MFNTHKVEIEWGGRPLTLETGKIARQADGAVLATYGETAVLATVVSAKEPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSENETLVSRLIDRPIRPLFVDGYKNDTQVVITVLQHDLENNPDILSMVAASAALTISGVPFMGPISGARVGYIDGE
YVLNPNIDEMPESKLDLVVAGTSEAVLMVESEAQELPEDVMLGAVMFGHKSFQPVIDAIIKLAEVAAKEPRDFQPEDLSE
LEAKVLAVVENDLREAYKITEKQARYAAVDAAKAKAEEHFFPEGVEETEMSAEQFATIFKHLQAKIVRWNILDTGNRIDG
RDLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGTGEDEQMIDALTGTYKESFMLHYNFPPYSVGETGRMGSPGR
REIGHGKLAWRAIHPMLPAAEQFPYTIRAVSEITESNGSSSMATVCGTSLALMDAGVPIVRPVAGIAMGLIKEGERFAVL
SDILGDEDHLGDMDFKVAGTEFGITSLQMDIKIDGITEEIMKVALEQAKGGRVHILGEMAKAISSSRAELGEFAPRIEVM
NIPTDKIRDVIGSGGKVIREIVEKTGAKINIEDDGTVKIASSNGKEIEAAKKWIHSIVAEPEVGEIYEGTVVKTADFGAF
VNFFGPRDGLVHISQLAADRVAKTTDVVKEGQKVWVKLMGFDERGKVRLSMKVVDQETGKEIVAEKKKEEVDAE
>Mature_714_residues
MFNTHKVEIEWGGRPLTLETGKIARQADGAVLATYGETAVLATVVSAKEPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSENETLVSRLIDRPIRPLFVDGYKNDTQVVITVLQHDLENNPDILSMVAASAALTISGVPFMGPISGARVGYIDGE
YVLNPNIDEMPESKLDLVVAGTSEAVLMVESEAQELPEDVMLGAVMFGHKSFQPVIDAIIKLAEVAAKEPRDFQPEDLSE
LEAKVLAVVENDLREAYKITEKQARYAAVDAAKAKAEEHFFPEGVEETEMSAEQFATIFKHLQAKIVRWNILDTGNRIDG
RDLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGTGEDEQMIDALTGTYKESFMLHYNFPPYSVGETGRMGSPGR
REIGHGKLAWRAIHPMLPAAEQFPYTIRAVSEITESNGSSSMATVCGTSLALMDAGVPIVRPVAGIAMGLIKEGERFAVL
SDILGDEDHLGDMDFKVAGTEFGITSLQMDIKIDGITEEIMKVALEQAKGGRVHILGEMAKAISSSRAELGEFAPRIEVM
NIPTDKIRDVIGSGGKVIREIVEKTGAKINIEDDGTVKIASSNGKEIEAAKKWIHSIVAEPEVGEIYEGTVVKTADFGAF
VNFFGPRDGLVHISQLAADRVAKTTDVVKEGQKVWVKLMGFDERGKVRLSMKVVDQETGKEIVAEKKKEEVDAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=725, Percent_Identity=38.3448275862069, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI4826690, Length=96, Percent_Identity=45.8333333333333, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI21361576, Length=86, Percent_Identity=45.3488372093023, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=58.5858585858586, Blast_Score=807, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=717, Percent_Identity=33.1938633193863, Blast_Score=339, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17535281, Length=84, Percent_Identity=46.4285714285714, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320850, Length=108, Percent_Identity=37.962962962963, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281362905, Length=722, Percent_Identity=37.6731301939058, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=722, Percent_Identity=37.6731301939058, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=722, Percent_Identity=37.6731301939058, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=666, Percent_Identity=37.0870870870871, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI20129977, Length=91, Percent_Identity=47.2527472527472, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77732; Mature: 77732

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNTHKVEIEWGGRPLTLETGKIARQADGAVLATYGETAVLATVVSAKEPKPGQDFFPLT
CCCCEEEEEEECCEEEEEECCCHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEE
VNYQEKTYAAGKIPGGYFKREGRPSENETLVSRLIDRPIRPLFVDGYKNDTQVVITVLQH
EECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHH
DLENNPDILSMVAASAALTISGVPFMGPISGARVGYIDGEYVLNPNIDEMPESKLDLVVA
HCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCEEEE
GTSEAVLMVESEAQELPEDVMLGAVMFGHKSFQPVIDAIIKLAEVAAKEPRDFQPEDLSE
CCCCEEEEECCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LEAKVLAVVENDLREAYKITEKQARYAAVDAAKAKAEEHFFPEGVEETEMSAEQFATIFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
HLQAKIVRWNILDTGNRIDGRDLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGT
HHHHEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECC
GEDEQMIDALTGTYKESFMLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIHPMLPAA
CCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCH
EQFPYTIRAVSEITESNGSSSMATVCGTSLALMDAGVPIVRPVAGIAMGLIKEGERFAVL
HHCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHH
SDILGDEDHLGDMDFKVAGTEFGITSLQMDIKIDGITEEIMKVALEQAKGGRVHILGEMA
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHH
KAISSSRAELGEFAPRIEVMNIPTDKIRDVIGSGGKVIREIVEKTGAKINIEDDGTVKIA
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEE
SSNGKEIEAAKKWIHSIVAEPEVGEIYEGTVVKTADFGAFVNFFGPRDGLVHISQLAADR
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCHHHHHHHHHHH
VAKTTDVVKEGQKVWVKLMGFDERGKVRLSMKVVDQETGKEIVAEKKKEEVDAE
HHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFNTHKVEIEWGGRPLTLETGKIARQADGAVLATYGETAVLATVVSAKEPKPGQDFFPLT
CCCCEEEEEEECCEEEEEECCCHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEE
VNYQEKTYAAGKIPGGYFKREGRPSENETLVSRLIDRPIRPLFVDGYKNDTQVVITVLQH
EECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHH
DLENNPDILSMVAASAALTISGVPFMGPISGARVGYIDGEYVLNPNIDEMPESKLDLVVA
HCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCEEEE
GTSEAVLMVESEAQELPEDVMLGAVMFGHKSFQPVIDAIIKLAEVAAKEPRDFQPEDLSE
CCCCEEEEECCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LEAKVLAVVENDLREAYKITEKQARYAAVDAAKAKAEEHFFPEGVEETEMSAEQFATIFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
HLQAKIVRWNILDTGNRIDGRDLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGT
HHHHEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECC
GEDEQMIDALTGTYKESFMLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIHPMLPAA
CCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCH
EQFPYTIRAVSEITESNGSSSMATVCGTSLALMDAGVPIVRPVAGIAMGLIKEGERFAVL
HHCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHH
SDILGDEDHLGDMDFKVAGTEFGITSLQMDIKIDGITEEIMKVALEQAKGGRVHILGEMA
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHH
KAISSSRAELGEFAPRIEVMNIPTDKIRDVIGSGGKVIREIVEKTGAKINIEDDGTVKIA
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEE
SSNGKEIEAAKKWIHSIVAEPEVGEIYEGTVVKTADFGAFVNFFGPRDGLVHISQLAADR
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCHHHHHHHHHHH
VAKTTDVVKEGQKVWVKLMGFDERGKVRLSMKVVDQETGKEIVAEKKKEEVDAE
HHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA