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The map label for this gene is truB [H]

Identifier: 225853592

GI number: 225853592

Start: 2107785

End: 2108759

Strand: Direct

Name: truB [H]

Synonym: BMEA_A2227

Alternate gene names: 225853592

Gene position: 2107785-2108759 (Clockwise)

Preceding gene: 225853591

Following gene: 225853593

Centisome position: 99.16

GC content: 60.62

Gene sequence:

>975_bases
ATGGCAAGACGGGGCAAGAAAAAAGGTCGCCCGATATCCGGCTGGGTCATTTTTGACAAGCCGAAGGGAATGGGATCGAC
CGAGGCGGTCTCGAAGATCAAGTGGCTTTTCAGTGCTGAAAAGGCTGGCCATGCGGGCACGCTCGATCCGCTTGCTTCGG
GCATGTTGCCCATCGCGCTTGGCGAAGCCACGAAGACGGTGCCTTATGCCATGGACGGAACCAAGGTCTATCGTTTTACG
GTGACATGGGGCGAGGAGCGTTCCACGGACGATCTGGAAGGCCAGCCCACCAAAACTTCCGACAAGCGTCCGTCGCGTGA
GGAAGTGGAAGCACTCCTGCCGGATTATACCGGCGTGATCTCGCAGGTTCCGCCGCAGTTTTCCGCAATCAAGATCGATG
GCGAGCGCGCTTATGATCTGGCCCGCGAGGGTGAAACCGTGGAAATTCCCGCCCGTGAGGTCGAGATCGACCGCCTTGAG
ATCGTAGGCTTTCCCGATGCCGACCGTACCGAATTTGAGGTGGAATGCTCCAAGGGTACCTATGTGCGCTCGCTTGCGCG
CGACATGGGGCGCGATCTCGGCTGCTATGGTCATATTTCCGATCTGCGCCGTGTGGAAGTTGCGCCCTTCACCGATGAAG
ATATGGTGACGCTCGCAAAGCTTGAGGCTGTCTGGCCGCCGCTTCCGCCGAAGGATGAGGACGGCAATGTGATCGAGCCT
GCACCGCGGCGCGATTTCTCCGCGCTTGATGCGCTGGTGATCGATACGGGTGCGGCACTTGATTGTCTGCCGCAAGTGCC
GCTCTCCGACGATCAGGCCCAGCGGGTGCGCCTTGGCAATCCGGTCATCCTGCGCGGGCGCGATGCGCCGCTGGAAGCCG
ACGAGGCCTGCGTTACCACGCGCGGCAAGCTTCTCGCCATCGGCTATATCGAGCACGGGCAGTTCAAGCCGAAACGGGTT
TTCACCGCCGGCTGA

Upstream 100 bases:

>100_bases
TGCGCTTCTCCGCTTCCCGGAAGTGGCGCGCGATCTGTCGCATGACGATGATGAAGATGGCGGAGCGGATGAAGCCCCGC
GCAATGGAGACGAATAAAGC

Downstream 100 bases:

>100_bases
CCGGCGCGCCGGGGGGCGGAATTTATTTCAGACTGGCATTCTTGTCGTTTTTGGACTATATGCCCCTGATCGATTGCGTG
AAAGAGCGTTCAAATCGTGT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MARRGKKKGRPISGWVIFDKPKGMGSTEAVSKIKWLFSAEKAGHAGTLDPLASGMLPIALGEATKTVPYAMDGTKVYRFT
VTWGEERSTDDLEGQPTKTSDKRPSREEVEALLPDYTGVISQVPPQFSAIKIDGERAYDLAREGETVEIPAREVEIDRLE
IVGFPDADRTEFEVECSKGTYVRSLARDMGRDLGCYGHISDLRRVEVAPFTDEDMVTLAKLEAVWPPLPPKDEDGNVIEP
APRRDFSALDALVIDTGAALDCLPQVPLSDDQAQRVRLGNPVILRGRDAPLEADEACVTTRGKLLAIGYIEHGQFKPKRV
FTAG

Sequences:

>Translated_324_residues
MARRGKKKGRPISGWVIFDKPKGMGSTEAVSKIKWLFSAEKAGHAGTLDPLASGMLPIALGEATKTVPYAMDGTKVYRFT
VTWGEERSTDDLEGQPTKTSDKRPSREEVEALLPDYTGVISQVPPQFSAIKIDGERAYDLAREGETVEIPAREVEIDRLE
IVGFPDADRTEFEVECSKGTYVRSLARDMGRDLGCYGHISDLRRVEVAPFTDEDMVTLAKLEAVWPPLPPKDEDGNVIEP
APRRDFSALDALVIDTGAALDCLPQVPLSDDQAQRVRLGNPVILRGRDAPLEADEACVTTRGKLLAIGYIEHGQFKPKRV
FTAG
>Mature_323_residues
ARRGKKKGRPISGWVIFDKPKGMGSTEAVSKIKWLFSAEKAGHAGTLDPLASGMLPIALGEATKTVPYAMDGTKVYRFTV
TWGEERSTDDLEGQPTKTSDKRPSREEVEALLPDYTGVISQVPPQFSAIKIDGERAYDLAREGETVEIPAREVEIDRLEI
VGFPDADRTEFEVECSKGTYVRSLARDMGRDLGCYGHISDLRRVEVAPFTDEDMVTLAKLEAVWPPLPPKDEDGNVIEPA
PRRDFSALDALVIDTGAALDCLPQVPLSDDQAQRVRLGNPVILRGRDAPLEADEACVTTRGKLLAIGYIEHGQFKPKRVF
TAG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=223, Percent_Identity=34.5291479820628, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI215599015, Length=224, Percent_Identity=29.9107142857143, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4503337, Length=224, Percent_Identity=29.9107142857143, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367200, Length=322, Percent_Identity=36.0248447204969, Blast_Score=188, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17553978, Length=224, Percent_Identity=30.8035714285714, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=233, Percent_Identity=29.6137339055794, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=134, Percent_Identity=35.8208955223881, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281364189, Length=308, Percent_Identity=26.2987012987013, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281364187, Length=308, Percent_Identity=26.2987012987013, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281364185, Length=308, Percent_Identity=26.2987012987013, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281364183, Length=308, Percent_Identity=26.2987012987013, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI62471759, Length=308, Percent_Identity=26.2987012987013, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17975520, Length=308, Percent_Identity=26.2987012987013, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35568; Mature: 35437

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRGKKKGRPISGWVIFDKPKGMGSTEAVSKIKWLFSAEKAGHAGTLDPLASGMLPIAL
CCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEEEE
GEATKTVPYAMDGTKVYRFTVTWGEERSTDDLEGQPTKTSDKRPSREEVEALLPDYTGVI
CCCCCCCCEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHH
SQVPPQFSAIKIDGERAYDLAREGETVEIPAREVEIDRLEIVGFPDADRTEFEVECSKGT
HHCCCCCEEEEECCHHHHHHHHCCCEEECCHHEEEEEEEEEEECCCCCCCEEEEEECCCH
YVRSLARDMGRDLGCYGHISDLRRVEVAPFTDEDMVTLAKLEAVWPPLPPKDEDGNVIEP
HHHHHHHHHCCCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEECC
APRRDFSALDALVIDTGAALDCLPQVPLSDDQAQRVRLGNPVILRGRDAPLEADEACVTT
CCCCCHHHHHEEEECCCCHHHHCCCCCCCCCCHHHEECCCEEEEECCCCCCCCCCHHEEC
RGKLLAIGYIEHGQFKPKRVFTAG
CCCEEEEEEEECCCCCCCEEEECC
>Mature Secondary Structure 
ARRGKKKGRPISGWVIFDKPKGMGSTEAVSKIKWLFSAEKAGHAGTLDPLASGMLPIAL
CCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEEEE
GEATKTVPYAMDGTKVYRFTVTWGEERSTDDLEGQPTKTSDKRPSREEVEALLPDYTGVI
CCCCCCCCEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHH
SQVPPQFSAIKIDGERAYDLAREGETVEIPAREVEIDRLEIVGFPDADRTEFEVECSKGT
HHCCCCCEEEEECCHHHHHHHHCCCEEECCHHEEEEEEEEEEECCCCCCCEEEEEECCCH
YVRSLARDMGRDLGCYGHISDLRRVEVAPFTDEDMVTLAKLEAVWPPLPPKDEDGNVIEP
HHHHHHHHHCCCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEECC
APRRDFSALDALVIDTGAALDCLPQVPLSDDQAQRVRLGNPVILRGRDAPLEADEACVTT
CCCCCHHHHHEEEECCCCHHHHCCCCCCCCCCHHHEECCCEEEEECCCCCCCCCCHHEEC
RGKLLAIGYIEHGQFKPKRVFTAG
CCCEEEEEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA