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The map label for this gene is metK [H]

Identifier: 225853585

GI number: 225853585

Start: 2098581

End: 2099846

Strand: Direct

Name: metK [H]

Synonym: BMEA_A2220

Alternate gene names: 225853585

Gene position: 2098581-2099846 (Clockwise)

Preceding gene: 225853584

Following gene: 225853586

Centisome position: 98.72

GC content: 60.43

Gene sequence:

>1266_bases
GTGTCGCGCAGTTCTTATCTCTTCACCAGCGAATCTGTGTCCGAAGGACACCCGGACAAGGTTTGCGACCGCATTTCCGA
TGAAATCGTTGATATGATCTACAAGGAGGCACGTCGCACCGGCGTCGATCCGTGGTCTGTCCGCATTGCCTGCGAAACGC
TTGCCACAACCAATCGTGTGGTCATTGCCGGCGAAGTACGCGTGCCGGAAACCTTCCTGAAGAAGAATAAGGACGGCTCC
ATCGCCCATGATGCAGCCGGTCATCCGCTCATCAACCCGTCCCGTTTCCGTTCGGCGGCGCGCAAGGCGATCCGCGAGAT
CGGCTATGAGCAGGATGGCTTCAACTGGCGCACGGTGAAGGTCGATGTTCTCCTGCATCCCCAATCGGCCGATATTGCGC
AGGGCGTGGACAATGCCGCCGACCGTCAGGGTGAGGAAGGCGCAGGCGATCAGGGCATCATGTTCGGCTATGCCTGCCGC
GAAACCCTGGACCTCATGCCGGCTCCGATCTATTATTCGCACAAGATTCTCGAAAAGCTCGCAGAAGCCCGCCACAAGGG
CGAGGGTGATGCCCGCAAGCTCGGCCCCGATGCCAAGAGCCAGGTGACGGTGCGTTATGAAAACGGCAAGGCTGCCGAAG
TCACGCAGATCGTCCTTTCCACGCAGCATCTCGATGCGTCCTGGGATTCCAGGAAGGTGCGTTCGGTCGTGGAACCCTAT
ATCCGCGAAGCCTTGGGCGATCTGCCGATTGCCGAAAACTGCAACTGGTACATCAATCCGACCGGCAAGTTCGTTATCGG
TGGACCGGACGGTGATGCGGGCCTGACAGGCCGCAAGATCATCGTTGACACCTATGGCGGCGCGGCCCCGCATGGCGGGG
GCGCTTTCTCCGGCAAGGATACGACCAAGGTGGATCGCTCCGCGGCCTATGCCGCGCGCTATCTCGCCAAGAATGTGGTT
GCCGCCGGCCTTGCCGACCGTTGCACCATCCAGCTTTCCTACGCCATCGGCGTTGCCCAGCCGCTTTCGGTCTATGTGGA
CCTGCACGGCACGGGCAAGGTCGCCGAATCCGCTGTCGAGGATGCGTTGCGCAAGGTGATGGACCTGTCCCCCACCGGCA
TCCGCAAGCATCTCGACCTCAACAAGCCGATCTACGCCAAGACCTCGTCCTACGGCCATTTTGGCCGCAAGCCGGGCCGT
GATGGTTCCTTCTCCTGGGAGAAGACCGATCTCATCAAGGCGTTGAAAGCGGCTGTTTCAGCCTGA

Upstream 100 bases:

>100_bases
CAACTTCGCTAACACATCTATGCGCCCGGTGAAGTCCGGGCGCTACTGTGTTTCCAAGGCGGAGCATTTTTCTCCGCTGG
ATTTTCAAGAGGGGTTACCC

Downstream 100 bases:

>100_bases
TTGACCCTATGATCGATGAAAATCATCCCATGCGCGCGGCCGGAAATTTTTTCGGCCGCCGCCACGGCAAGCCGCTCCGT
CCGCACCAGAGCAATCTGTT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSRSSYLFTSESVSEGHPDKVCDRISDEIVDMIYKEARRTGVDPWSVRIACETLATTNRVVIAGEVRVPETFLKKNKDGS
IAHDAAGHPLINPSRFRSAARKAIREIGYEQDGFNWRTVKVDVLLHPQSADIAQGVDNAADRQGEEGAGDQGIMFGYACR
ETLDLMPAPIYYSHKILEKLAEARHKGEGDARKLGPDAKSQVTVRYENGKAAEVTQIVLSTQHLDASWDSRKVRSVVEPY
IREALGDLPIAENCNWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVV
AAGLADRCTIQLSYAIGVAQPLSVYVDLHGTGKVAESAVEDALRKVMDLSPTGIRKHLDLNKPIYAKTSSYGHFGRKPGR
DGSFSWEKTDLIKALKAAVSA

Sequences:

>Translated_421_residues
MSRSSYLFTSESVSEGHPDKVCDRISDEIVDMIYKEARRTGVDPWSVRIACETLATTNRVVIAGEVRVPETFLKKNKDGS
IAHDAAGHPLINPSRFRSAARKAIREIGYEQDGFNWRTVKVDVLLHPQSADIAQGVDNAADRQGEEGAGDQGIMFGYACR
ETLDLMPAPIYYSHKILEKLAEARHKGEGDARKLGPDAKSQVTVRYENGKAAEVTQIVLSTQHLDASWDSRKVRSVVEPY
IREALGDLPIAENCNWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVV
AAGLADRCTIQLSYAIGVAQPLSVYVDLHGTGKVAESAVEDALRKVMDLSPTGIRKHLDLNKPIYAKTSSYGHFGRKPGR
DGSFSWEKTDLIKALKAAVSA
>Mature_420_residues
SRSSYLFTSESVSEGHPDKVCDRISDEIVDMIYKEARRTGVDPWSVRIACETLATTNRVVIAGEVRVPETFLKKNKDGSI
AHDAAGHPLINPSRFRSAARKAIREIGYEQDGFNWRTVKVDVLLHPQSADIAQGVDNAADRQGEEGAGDQGIMFGYACRE
TLDLMPAPIYYSHKILEKLAEARHKGEGDARKLGPDAKSQVTVRYENGKAAEVTQIVLSTQHLDASWDSRKVRSVVEPYI
REALGDLPIAENCNWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVA
AGLADRCTIQLSYAIGVAQPLSVYVDLHGTGKVAESAVEDALRKVMDLSPTGIRKHLDLNKPIYAKTSSYGHFGRKPGRD
GSFSWEKTDLIKALKAAVSA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=419, Percent_Identity=45.5847255369928, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI4557737, Length=414, Percent_Identity=44.4444444444444, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1789311, Length=417, Percent_Identity=48.2014388489209, Blast_Score=355, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI17538498, Length=415, Percent_Identity=44.0963855421687, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17538494, Length=415, Percent_Identity=44.3373493975904, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25145633, Length=420, Percent_Identity=43.5714285714286, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17551082, Length=420, Percent_Identity=43.3333333333333, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI32565911, Length=320, Percent_Identity=47.8125, Blast_Score=248, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI25145635, Length=325, Percent_Identity=46.7692307692308, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=48.8448844884488, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI32565905, Length=301, Percent_Identity=49.1694352159468, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI32565909, Length=66, Percent_Identity=46.969696969697, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320710, Length=419, Percent_Identity=44.6300715990453, Blast_Score=301, Evalue=9e-83,
Organism=Saccharomyces cerevisiae, GI6323209, Length=418, Percent_Identity=44.4976076555024, Blast_Score=298, Evalue=7e-82,
Organism=Drosophila melanogaster, GI24580527, Length=415, Percent_Identity=44.3373493975904, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24580525, Length=415, Percent_Identity=44.3373493975904, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI45552159, Length=415, Percent_Identity=44.578313253012, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24580529, Length=415, Percent_Identity=44.578313253012, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24580537, Length=415, Percent_Identity=44.578313253012, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24580533, Length=415, Percent_Identity=44.578313253012, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24580535, Length=415, Percent_Identity=44.578313253012, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24580531, Length=415, Percent_Identity=44.578313253012, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24580541, Length=414, Percent_Identity=42.512077294686, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24580539, Length=151, Percent_Identity=35.0993377483444, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 45691; Mature: 45560

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSSYLFTSESVSEGHPDKVCDRISDEIVDMIYKEARRTGVDPWSVRIACETLATTNRV
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEE
VIAGEVRVPETFLKKNKDGSIAHDAAGHPLINPSRFRSAARKAIREIGYEQDGFNWRTVK
EEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEE
VDVLLHPQSADIAQGVDNAADRQGEEGAGDQGIMFGYACRETLDLMPAPIYYSHKILEKL
EEEEECCCCCHHHHCCCHHHHCCCCCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHH
AEARHKGEGDARKLGPDAKSQVTVRYENGKAAEVTQIVLSTQHLDASWDSRKVRSVVEPY
HHHHHCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
IREALGDLPIAENCNWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKD
HHHHHCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
TTKVDRSAAYAARYLAKNVVAAGLADRCTIQLSYAIGVAQPLSVYVDLHGTGKVAESAVE
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHH
DALRKVMDLSPTGIRKHLDLNKPIYAKTSSYGHFGRKPGRDGSFSWEKTDLIKALKAAVS
HHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure 
SRSSYLFTSESVSEGHPDKVCDRISDEIVDMIYKEARRTGVDPWSVRIACETLATTNRV
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEE
VIAGEVRVPETFLKKNKDGSIAHDAAGHPLINPSRFRSAARKAIREIGYEQDGFNWRTVK
EEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEE
VDVLLHPQSADIAQGVDNAADRQGEEGAGDQGIMFGYACRETLDLMPAPIYYSHKILEKL
EEEEECCCCCHHHHCCCHHHHCCCCCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHH
AEARHKGEGDARKLGPDAKSQVTVRYENGKAAEVTQIVLSTQHLDASWDSRKVRSVVEPY
HHHHHCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
IREALGDLPIAENCNWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKD
HHHHHCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
TTKVDRSAAYAARYLAKNVVAAGLADRCTIQLSYAIGVAQPLSVYVDLHGTGKVAESAVE
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHH
DALRKVMDLSPTGIRKHLDLNKPIYAKTSSYGHFGRKPGRDGSFSWEKTDLIKALKAAVS
HHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA