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The map label for this gene is phoB [H]

Identifier: 225853570

GI number: 225853570

Start: 2084805

End: 2085491

Strand: Direct

Name: phoB [H]

Synonym: BMEA_A2204

Alternate gene names: 225853570

Gene position: 2084805-2085491 (Clockwise)

Preceding gene: 225853569

Following gene: 225853572

Centisome position: 98.08

GC content: 62.01

Gene sequence:

>687_bases
ATGTCCCAAGTACCCCGAATTGCTGTGGTTGAAGACGAAGAAGCACTGAGCGTGCTGCTGCGCTATAATCTCGAAGCTGA
GGGCTATCAGGTCGATACGATGTTGCGCGGGGATGAAGCCGAAATCGCGCTGCGTGAAAGCGTGCCCGATCTTCTGATCC
TGGACTGGATGCTGCCGGGCGTCTCCGGCATCGAGCTTTGCCGCCGCCTGCGGCAAAGACCGGAAACGGAACGCCTGCCG
ATCATCATGCTGACGGCGCGCGGCGAGGAGAGCGAGCGTGTACGGGGCCTGAGTGTCGGCGCGGATGATTATGTGGTGAA
GCCTTTCTCCACGCCGGAGCTGCTTGCCCGCGTCAAGGCGATGCTGCGCCGCGCGAACCCCAGCATCCTGTCCCATGTGC
TGAAGGTCGGCGACCTTTTGCTGGACCGCCAGCAGCACCGCGTCTATCGGAAGGAAAAGGAAGTCCACCTTGGCCCGACG
GAATTCCGTCTGCTCGAATATTTCATGATGTCGCCGGGCCGCGTTTTCTCGCGCAGCCAGCTTCTGGATGGTGTCTGGGG
GCCGGATATCTACGTCGATGACCGCACGGTAGACGTCCATGTGGGCCGTCTGCGCAAGGCCATCAATGTCGGCCGCGCCG
CCGATCCGATCCGCACCGTACGCGGCGCGGGATATTCGTTCGGCTGA

Upstream 100 bases:

>100_bases
TTGAACCCGACGGCTGAGAATGAGTGTACGGCATGGCGGCACGCGGTCGATAGGGGCTGGTATCCTGCGCTCGCCTGAAA
TCTCGAGAGGGAAAGATTGC

Downstream 100 bases:

>100_bases
CGGGAGCGGTTCCCCCTTTCAGGCGGCCCGTTTTTCCTCCGGGAAGCGGGCTTGCACAGGCTTGCGATTCGCTTTTCCGG
TGGGTGGAACTGGTGATTCA

Product: phosphate regulon transcriptional regulatory protein PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSQVPRIAVVEDEEALSVLLRYNLEAEGYQVDTMLRGDEAEIALRESVPDLLILDWMLPGVSGIELCRRLRQRPETERLP
IIMLTARGEESERVRGLSVGADDYVVKPFSTPELLARVKAMLRRANPSILSHVLKVGDLLLDRQQHRVYRKEKEVHLGPT
EFRLLEYFMMSPGRVFSRSQLLDGVWGPDIYVDDRTVDVHVGRLRKAINVGRAADPIRTVRGAGYSFG

Sequences:

>Translated_228_residues
MSQVPRIAVVEDEEALSVLLRYNLEAEGYQVDTMLRGDEAEIALRESVPDLLILDWMLPGVSGIELCRRLRQRPETERLP
IIMLTARGEESERVRGLSVGADDYVVKPFSTPELLARVKAMLRRANPSILSHVLKVGDLLLDRQQHRVYRKEKEVHLGPT
EFRLLEYFMMSPGRVFSRSQLLDGVWGPDIYVDDRTVDVHVGRLRKAINVGRAADPIRTVRGAGYSFG
>Mature_227_residues
SQVPRIAVVEDEEALSVLLRYNLEAEGYQVDTMLRGDEAEIALRESVPDLLILDWMLPGVSGIELCRRLRQRPETERLPI
IMLTARGEESERVRGLSVGADDYVVKPFSTPELLARVKAMLRRANPSILSHVLKVGDLLLDRQQHRVYRKEKEVHLGPTE
FRLLEYFMMSPGRVFSRSQLLDGVWGPDIYVDDRTVDVHVGRLRKAINVGRAADPIRTVRGAGYSFG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=50.2242152466368, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=43.6123348017621, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=40.5286343612335, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1786911, Length=230, Percent_Identity=39.1304347826087, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=39.0909090909091, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1789809, Length=238, Percent_Identity=36.5546218487395, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=37.6681614349776, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=36.7713004484305, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=36.322869955157, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=36.8181818181818, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=35.042735042735, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI2367329, Length=228, Percent_Identity=37.280701754386, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=33.4782608695652, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI145693140, Length=237, Percent_Identity=32.0675105485232, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=32.4561403508772, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=32.7433628318584, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25878; Mature: 25747

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVPRIAVVEDEEALSVLLRYNLEAEGYQVDTMLRGDEAEIALRESVPDLLILDWMLPG
CCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCHHEEHHHCCCCCEEHHHHCCC
VSGIELCRRLRQRPETERLPIIMLTARGEESERVRGLSVGADDYVVKPFSTPELLARVKA
CCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHEEEEECCCCEEECCCCCHHHHHHHHH
MLRRANPSILSHVLKVGDLLLDRQQHRVYRKEKEVHLGPTEFRLLEYFMMSPGRVFSRSQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHH
LLDGVWGPDIYVDDRTVDVHVGRLRKAINVGRAADPIRTVRGAGYSFG
HHCCCCCCCEEECCCEEEHHHHHHHHHHHCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SQVPRIAVVEDEEALSVLLRYNLEAEGYQVDTMLRGDEAEIALRESVPDLLILDWMLPG
CCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCHHEEHHHCCCCCEEHHHHCCC
VSGIELCRRLRQRPETERLPIIMLTARGEESERVRGLSVGADDYVVKPFSTPELLARVKA
CCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHEEEEECCCCEEECCCCCHHHHHHHHH
MLRRANPSILSHVLKVGDLLLDRQQHRVYRKEKEVHLGPTEFRLLEYFMMSPGRVFSRSQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHH
LLDGVWGPDIYVDDRTVDVHVGRLRKAINVGRAADPIRTVRGAGYSFG
HHCCCCCCCEEECCCEEEHHHHHHHHHHHCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]