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The map label for this gene is afr [H]

Identifier: 225853479

GI number: 225853479

Start: 1991180

End: 1992181

Strand: Reverse

Name: afr [H]

Synonym: BMEA_A2109

Alternate gene names: 225853479

Gene position: 1992181-1991180 (Counterclockwise)

Preceding gene: 225853480

Following gene: 225853478

Centisome position: 93.72

GC content: 59.98

Gene sequence:

>1002_bases
ATGGCAAAGTGGGGCCTTATAGGCGCAAGCACCATTGCAAAGGAATGGGTGATCGGCGCCATTCGCGCGACGGGTGGCGA
GGTCGTTTCCGTCTACAGCACCAATGCCGCGCGCGGTAAAACCTATGCAGACGAGAACGGCATCGCCCGTTCGGTGACGA
GCCTCGATGCGCTGCTGGCCGACCCGGAAATAGACGCGGTTTATATCTCCACCACCAATGAACTGCATCGCGATCAGGCC
ATTGCCGCGGCACAAGCGGGCAAGCATATCCTGTGCGAAAAGCCGCTGGCGCTGACGATTGGTGACGCTCATGAAATGCT
GAAAGCGGCGCGCGCAGCGGGCGTGGTGGCAGGCACCAATCATCATTTGCGCAATGCGGCAAGCCACCGCGCCATGCGCG
ATGCCGTAGCAGAAGGCCGGATCGGCAAGGTGCTGGGCGCGCGGGTTTTCCATGCGGTTTATTTGCCGCCACACTTACAG
GGCTGGCGCATTGAGCGGCCCGATGCGGGCGGCGGTGTCGTCCTCGATATCACTGTGCATGATGCCGATACGCTGCGTTT
TGTGCTGGGGGAAAATCCGGTCGAGGCGGTCGCTTTCAGTCAACAGGGCGGCTTAAGTGGAGAAGGGCTGGAAGATGGCG
TGATGGGCGTTTTGCGTTTTCCATCCGGGGCGATTGCCCAGTTTCACGATGCTTTCACCGCCAAATATGCCGAAACCGGT
TTCGGGGTGCATGGCAGCGAAGGTTCGCTCATTGCGCGCAATGTGATGACGCAAAAGCCGGTCGGGACCGTCATCCTGCG
GGACAAGGACGGCGAACACGAATTGCCGCTCGACCACAAAAATCTCTACGAAACCGCCTTGCAGGCTTTTCACGATGCCA
TTGCCGGAAGAGGCCAGCCATCGGCCACATTGGAGGATGGCATCTGGTCGCTCGCCACCGGGCTGGCCGTGCTTGAGGCC
GCAAAAACCGGCAAAGCCACCGCCGTTCATTCAGGACTTTAA

Upstream 100 bases:

>100_bases
ATTCGGAACGGGTGGAGGCGGAGGCGCAGCGTCTGTCGGTGCGTCTTGCAGTGCGTGCAAGTTGCGGCGCGCCGTTCAGA
AAACCGGAGGAGAAATTTGA

Downstream 100 bases:

>100_bases
GACTTATGAGCAAGCATATTTCATTTGCTGAAGCCGCTGCACTTATCCCCGACAATGCGGTTGTTTCGGTTTCCTCGTCC
AGTGGCCTCGGTTGCCCGGA

Product: hypothetical protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAKWGLIGASTIAKEWVIGAIRATGGEVVSVYSTNAARGKTYADENGIARSVTSLDALLADPEIDAVYISTTNELHRDQA
IAAAQAGKHILCEKPLALTIGDAHEMLKAARAAGVVAGTNHHLRNAASHRAMRDAVAEGRIGKVLGARVFHAVYLPPHLQ
GWRIERPDAGGGVVLDITVHDADTLRFVLGENPVEAVAFSQQGGLSGEGLEDGVMGVLRFPSGAIAQFHDAFTAKYAETG
FGVHGSEGSLIARNVMTQKPVGTVILRDKDGEHELPLDHKNLYETALQAFHDAIAGRGQPSATLEDGIWSLATGLAVLEA
AKTGKATAVHSGL

Sequences:

>Translated_333_residues
MAKWGLIGASTIAKEWVIGAIRATGGEVVSVYSTNAARGKTYADENGIARSVTSLDALLADPEIDAVYISTTNELHRDQA
IAAAQAGKHILCEKPLALTIGDAHEMLKAARAAGVVAGTNHHLRNAASHRAMRDAVAEGRIGKVLGARVFHAVYLPPHLQ
GWRIERPDAGGGVVLDITVHDADTLRFVLGENPVEAVAFSQQGGLSGEGLEDGVMGVLRFPSGAIAQFHDAFTAKYAETG
FGVHGSEGSLIARNVMTQKPVGTVILRDKDGEHELPLDHKNLYETALQAFHDAIAGRGQPSATLEDGIWSLATGLAVLEA
AKTGKATAVHSGL
>Mature_332_residues
AKWGLIGASTIAKEWVIGAIRATGGEVVSVYSTNAARGKTYADENGIARSVTSLDALLADPEIDAVYISTTNELHRDQAI
AAAQAGKHILCEKPLALTIGDAHEMLKAARAAGVVAGTNHHLRNAASHRAMRDAVAEGRIGKVLGARVFHAVYLPPHLQG
WRIERPDAGGGVVLDITVHDADTLRFVLGENPVEAVAFSQQGGLSGEGLEDGVMGVLRFPSGAIAQFHDAFTAKYAETGF
GVHGSEGSLIARNVMTQKPVGTVILRDKDGEHELPLDHKNLYETALQAFHDAIAGRGQPSATLEDGIWSLATGLAVLEAA
KTGKATAVHSGL

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=281, Percent_Identity=25.6227758007117, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI87082405, Length=227, Percent_Identity=30.3964757709251, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787574, Length=326, Percent_Identity=27.3006134969325, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081947, Length=255, Percent_Identity=27.4509803921569, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI145693182, Length=214, Percent_Identity=24.2990654205607, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581117, Length=287, Percent_Identity=25.7839721254355, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24581115, Length=197, Percent_Identity=28.4263959390863, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24584727, Length=276, Percent_Identity=23.5507246376812, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 34950; Mature: 34819

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKWGLIGASTIAKEWVIGAIRATGGEVVSVYSTNAARGKTYADENGIARSVTSLDALLA
CCCCCCCCHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
DPEIDAVYISTTNELHRDQAIAAAQAGKHILCEKPLALTIGDAHEMLKAARAAGVVAGTN
CCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHHHHHCCEEECCC
HHLRNAASHRAMRDAVAEGRIGKVLGARVFHAVYLPPHLQGWRIERPDAGGGVVLDITVH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCEEECCCCCCCEEEEEEEE
DADTLRFVLGENPVEAVAFSQQGGLSGEGLEDGVMGVLRFPSGAIAQFHDAFTAKYAETG
CCCEEEEEECCCCHHEEEECCCCCCCCCCCCHHCEEEEECCCCHHHHHHHHHHHHHHHCC
FGVHGSEGSLIARNVMTQKPVGTVILRDKDGEHELPLDHKNLYETALQAFHDAIAGRGQP
CCEECCCCCEEEHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
SATLEDGIWSLATGLAVLEAAKTGKATAVHSGL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AKWGLIGASTIAKEWVIGAIRATGGEVVSVYSTNAARGKTYADENGIARSVTSLDALLA
CCCCCCCHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
DPEIDAVYISTTNELHRDQAIAAAQAGKHILCEKPLALTIGDAHEMLKAARAAGVVAGTN
CCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHHHHHCCEEECCC
HHLRNAASHRAMRDAVAEGRIGKVLGARVFHAVYLPPHLQGWRIERPDAGGGVVLDITVH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCEEECCCCCCCEEEEEEEE
DADTLRFVLGENPVEAVAFSQQGGLSGEGLEDGVMGVLRFPSGAIAQFHDAFTAKYAETG
CCCEEEEEECCCCHHEEEECCCCCCCCCCCCHHCEEEEECCCCHHHHHHHHHHHHHHHCC
FGVHGSEGSLIARNVMTQKPVGTVILRDKDGEHELPLDHKNLYETALQAFHDAIAGRGQP
CCEECCCCCEEEHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
SATLEDGIWSLATGLAVLEAAKTGKATAVHSGL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA